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. 2020 Feb 21;9(2):496. doi: 10.3390/cells9020496

Figure 1.

Figure 1

Extracellular nicotinamide phosphoribosyltrasferase (eNAMPT) binds to C-C chemokine receptor type 5 (CCR5) in HeLa cancer cells. (A) Representative flow cytometry analysis of CCR5 expression in HeLa-SCR and HeLa-CCR5 cells, using Rat anti-mouse CCR5 antibody. (B) Western blot analysis of CCR5 expression in HeLa-SCR and HeLa-CCR5 cells. The CCR5 antibody recognizes both human endogenous CCR5 and murine exogenous CCR5. (C) Representative FACS analysis and calculated percentage of positive cells of Rantes-PE (16 nM) binding on HeLa-CCR5 cells incubated in the presence or absence of eNAMPT (2.25 µM) or maraviroc (10 µM). Mean ± S.E.M. of five separate experiments; *** p < 0.001.