Figure 3.

Susd4 mRNA is Expressed in Neuronal Cells in WT Brain

The RNAscope Multiplex Fluorescent Assay was performed with sagittal brain sections from WT and Susd4 KO mice. Sets of probes were used to detect mRNA expression of Susd4 (green), NeuN (red), and Gfap (cyan) in situ. Nuclei were visualized with DAPI staining (blue).

(A) Representative images from cortex sections show the assay specifically detects Susd4 mRNA in WT but not in Susd4 KO, whereas NeuN mRNA is detected in both. Scale bar, 20 μm.

(B) Susd4 mRNA is highly expressed in the hippocampus. Scale bar, 100 μm. CA, cornu ammonis; DG, dentate gyrus.

(C and D) Representative higher-magnification views show that Susd4 mRNA colocalizes with NeuN (C) but not Gfap (D). Scale bar, 10 μm.

(E) Susd4mRNA is highly expressed in Purkinje cells not the granular cells in cerebellum. Scale bar, 40 μm. PCL, Purkinje cell layer; GL, granular layer.

(F) Representative higher-magnification view shows the high expression of Susd4 mRNA in Purkinje neurons. Scale bar, 10 μm.