Figure 3.
DNA complexation ability, transfection efficiency, and physico-chemical characteristics of pDNA/lPEI complexes prepared in 10 mM Hepes at different N/Ps. (a) Transfection efficiency (black bars) in L929 cells of pDNA/lPEI complexes prepared at different N/Ps. Results are expressed as luminescence signal (RLU) normalized to the total protein content in each cell lysate, and the DNA complexation ability of lPEI (red dots, solid line), evaluated by monitoring the fluorochrome exclusion from complexes as a function of the N/P. (b) Mean hydrodynamic diameter (DH, black dots solid line) and overall surface charge (ζP, red squares and dotted line) of pDNA/lPEI complexes at different N/Ps, as measured by dynamic light scattering (DLS) and electrophoretic light scattering (ELS), respectively. Results are expressed as mean ± SD (n ≥ 3).