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. 2020 Feb 8;12(2):187. doi: 10.3390/v12020187

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Effect of PRRSV infection or PRRSV-ADE infection on protein expression of cytokines in porcine AMs. Cytokine protein levels in culture supernatants of mock cells, poly (I:C)-stimulated cells, PRRSV-infected cells, PRRSV+PNI-infected cells or PRRSV+ICs-infected cells were detected by using ELISA Kits. (a) IFN-α protein level, (b) TNF-α protein level, (c) IL-10 protein level. *** p < 0.001, ** p < 0.01, * p < 0.05, ns: no significance. Note: PRRSV+PNI: PRRSV+porcine-negative IgG; PRRSV+ICs: PRRSV+porcine IgG against PRRSV; PRRSV-ADE: antibody-dependent enhancement of PRRSV.

Effect of PRRSV infection or PRRSV-ADE infection on protein expression of cytokines in porcine AMs. Cytokine protein levels in culture supernatants of mock cells, poly (I:C)-stimulated cells, PRRSV-infected cells, PRRSV+PNI-infected cells or PRRSV+ICs-infected cells were detected by using ELISA Kits. (a) IFN-α protein level, (b) TNF-α protein level, (c) IL-10 protein level. *** p < 0.001, ** p < 0.01, * p < 0.05, ns: no significance. Note: PRRSV+PNI: PRRSV+porcine-negative IgG; PRRSV+ICs: PRRSV+porcine IgG against PRRSV; PRRSV-ADE: antibody-dependent enhancement of PRRSV.