HBCs were infected with MHV-68 for 1 h, fresh media was added, and then HBCs were incubated for an additional 48 h in SFM. Uninfected HBC CM (−MHV) was used as control. Levels of secreted IL-1β, quantitated in seven independent experiments, were determined by ELISA. Results are presented as medians and percentiles; the lines inside the box indicate the median, the ends of the box describe the lower and upper quartiles, and the whiskers define the smallest and largest values. *p<0.001 versus uninfected control.