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. Author manuscript; available in PMC: 2021 Feb 1.
Published in final edited form as: Placenta. 2020 Jan 25;91:59–65. doi: 10.1016/j.placenta.2020.01.010

Figure 4. Effect of IL-1β blocking antibody on HUVEC IL-8 secretion and levels of HUVEC IL-8, E-selectin, ICAM-1, and VCAM-1 mRNA in response to CM from MHV-infected HBCs.

Figure 4.

(A) HUVECs were incubated with CM from MHV-infected HBCs that were either pretreated with an IL-1β blocking antibody (IL1β Ab), an isotype-matched control antibody (IgG), or no pretreatment (CM +MHV) (Panel A). Results are expressed as a mean + S.E.M. from four independent experiments. *p<0.05 versus CM+MHV and IgG control groups. (B) HUVECs were treated as described above, and levels of mRNA were analyzed by qPCR and normalized to 18S RNA levels. Results are expressed as a mean + S.E.M. or as a median and percentiles from five independent experiments. *p< 0.05 versus CM+MHV and IgG control groups.