Fig. 4. Mouse otoferlin FerA increased the muscle transduction of AAV.
2×109 vg of AAV9/luc, AAV8/luc, or AAV6/luc particles were first incubated with 1 μg mOto-FerA for 2 h at 4 °C, then administered via intramuscular injection in the left legs (face-up) in 6-week-old female C57BL/6 mice (n = 5). As the internal control, AAV vectors only were injected into the right legs (face-up). Several weeks (W1, W2, and W3) post AAV application, in vivo luminescence imaging was performed (A, D, and G). The photon signal (B, E, and H) and gene copy numbers (C, F, and I) in the leg muscle were measured and calculated. The data of each group represent the average and SD from five mice. *** p < 0.001, **p <0.01, and *p < 0.05 compared to control mice with AAV/luc treatment only. R: right leg, L: left leg.