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. 2020 Feb 5;9:e51247. doi: 10.7554/eLife.51247

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© 2020, Williams et al

This article is distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use and redistribution provided that the original author and source are credited.

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Figure 2. — (a) Growth kinetics of N. meningitidis wild-type, ΔltgA, ΔltgA^ltgA and ΔltgA^ltgA^Δ30 strains. Data represent three independent experiments. (b) Exponentially grown bacteria were treated with chloramphenicol (2 μg/ml) to block protein synthesis and survey the stability of LtgA for the indicated periods of time (in hours). Immunoblots were performed probing with anti-LtgA antibody. The expression of the outer membrane protein fHbp was used as an loading control. (c) The levels of LtgA over the time were analyzed and plotted as a stability curve by quantifying the band intensities using ImageJ software. For each strain, the LtgA intensity at time zero is referred to as 100%, while the simultaneously fHbp was used for loading control.

Figure 2—source data 1. Figure 2a – Analysis associated with growth kinetics of N. meningitidis wild-type, ΔltgA, ΔltgA ^ltgA and ΔltgA ^ltgAΔ30 strains.

elife-51247-fig2-data1.xlsx^{(71.4KB, xlsx)}

Figure 2—source data 2. Figure 2c – The stability of LtgA over time.

elife-51247-fig2-data2.xlsx^{(63.7KB, xlsx)}