(a) Strains (8013) expressing wild-type lytic transglycosylase were imaged using fluorescent microscopy (right panel) and scanning electron microscopy (SEM) (left panel). (b) Strains (8013) expressing mutant lytic transglycosylases (E481A) were imaged by fluorescent microscopy (right panel) and scanning electron microscopy (SEM) (left panel). (c) Strains (MC58) expressing wild-type lytic transglycosylase were imaged using fluorescent microscopy (right panel) and scanning electron microscopy (SEM) (left panel), (d–e) The Δape1 strains were imaged using fluorescent microscopy (right panel) and scanning electron microscopy (SEM) (left panel). White arrows in the images points to, irregular cell surfaces, high-molecular-weight blebs (not observed in other strains), asymmetrical diplococci, and lysed bacteria.