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. 2020 Feb 5;9:e51247. doi: 10.7554/eLife.51247

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© 2020, Williams et al

This article is distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use and redistribution provided that the original author and source are credited.

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Figure 5. — (a) Chromatogram of the size exclusion analysis of purified (panel 1) LtgA, (panel 2)Ape1, (panel 3) Ape1-LtgA 105 kDa. Overlay of the chromatograms corresponding to purified LtgA, Ape1, and LtgA-Ape1 protein complex (panel 4). Each insert represents SDS-PAGE analysis of peak fractions containing proteins. Lanes are labeled with corresponding volumes. (b) Ape1 stimulates the activity of LtgA toward the acetylated PG of N. meningitidis. (c) LtgA stimulates and stabilizes Ape1 activity toward p-nitrophenol acetate. Ape1 utilizes p-nitrophenol acetate as a substratewhile LtgA does not. Error bars show the standard deviation of triplicate.

Figure 5—source data 1. Figure 5b – LtgA stimulation assay.

elife-51247-fig5-data1.xlsx^{(945.3KB, xlsx)}

Figure 5—source data 2. Figure 5c – Ape1 stimulation assay.

elife-51247-fig5-data2.xlsx^{(67.3KB, xlsx)}

Figure 5—figure supplement 1. — (a) Chromatogram of the size exclusion analysis of purified (panel 1) LtgA, (panel 2)Ape1, (panel 3) Ape1-LtgA 105 kDa. Overlay of the chromatograms corresponding to purified LtgA, Ape1, and LtgA-Ape1 protein complex (panel 4). Each insert represents SDS-PAGE analysis of peak fractions containing proteins. Lanes are labeled with corresponding volumes. (b) Ape1 stimulates the activity of LtgA toward the acetylated PG of N. meningitidis. (c) LtgA stimulates and stabilizes Ape1 activity toward p-nitrophenol acetate. Ape1 utilizes p-nitrophenol acetate as a substratewhile LtgA does not. Error bars show the standard deviation of triplicate.

Figure 5—source data 1. Figure 5b – LtgA stimulation assay.

elife-51247-fig5-data1.xlsx^{(945.3KB, xlsx)}

Figure 5—source data 2. Figure 5c – Ape1 stimulation assay.

elife-51247-fig5-data2.xlsx^{(67.3KB, xlsx)}