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. 2020 Mar 20;9(3):36. doi: 10.1038/s41389-020-0220-5

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — a RNF187 knocking down efficiency in TNBC cell lines. BT549 cells were transfected with RNF187 siRNA. The knockdown efficiency was measured via western blot. b, c RNF187 depletion promotes TNBC cell migration in BT549 cells. Two independent siRNA were used in the study. Transwell was used to check the migration capacity. The cell number was counted and Data are presented as ±SD. **P < 0.01, ***P < 0.001 (student’s t-test). d, e RNF187 depletion promotes TNBC cell migration in MDAMB231 cells. Two independent siRNA were used in the study. Transwell was used to check the migration capacity. The cell number was counted and Data are presented as ±SD. **P < 0.01, ***P < 0.001 (student’s t-test). f, g Wound-healing assay of BT549 cells were transfected with indicated 50 nM RNF187 siRNA (mix of #1 and #2) or 50 nM control siRNA. Quantification of wound closure at the indicated time points. Data are presented as ± SD. **P < 0.01, ***P < 0.001 (student’s t-test). h, i Wound-healing assay of MDAMB231 cells were transfected with indicated 50 nM RNF187 siRNA (mix of #1 and #2) or 50 nM control siRNA. Quantification of wound closure at the indicated time points. Data are presented as ± SD. **P < 0.01, ***P < 0.001 (student’s t-test). j, k RNF187 depletion inhibits TNBC cancer cell proliferation. BT549 and MDAMB231 cells were transfected with siControl or siRNF187. After 24 h, the WST assay was used to determine the cellar metabolic activity at indicated time points after infection. Experiments were done in triplicates. *P < 0.05; **P < 0.01; ***P < 0.001 for cell growth comparison.