KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| Guinea pig polyclonal anti-vGluT1 (CU1705) | This study | RRID: AB_2750940 |
| Guinea pig polyclonal anti-vGluT1 (CU1706) | This study | RRID: AB_2665455 |
| Guinea pig polyclonal anti-vGluT1 (CU1707) | This study | RRID: AB_2750941 |
| Rabbit polyclonal anti-dsRed | Clontech | Cat#632496 |
| Goat polyclonal anti-Choline Acetyltransferase | Millipore | Cat#AB144P |
| Chicken polyclonal anti-GFP | Abcam | Cat#AB13970 |
| Rabbit polyclonal anti-GFP | Invitrogen | Cat#A11122 |
| Sheep polyclonal anti-GFP | AbD Serotec | Cat#4745–1051 |
| Goat polyclonal anti-Cholera Toxin, B-Subunit | List Biologicals | RRID: AB_10013220; Cat#703 |
| Mouse monoclonal anti-Cholera Toxin, B-Subunit | Abcam | Cat#AB35988 |
| Bacterial and Virus Strains | ||
| scAAV6-CBh-GFP | UNC Vector Core (VC) | scAAV6-CBh-GFP |
| scAAV6-CMV-Cre | This study | This study/ Custom UNC VC packaging |
| scAAVI -CMV-Cre | This study | This study/ Custom UNC VC packaging |
| scAAV9-CMV-Cre | This study | This study/ Custom UNC VC packaging |
| Rabies SAD-B19 ΔG-GFP | Wickersham et al., 2007 | N/A |
| Chemical, Peptides, and Recombinant Proteins | ||
| Cholera Toxin B Subunit (low salt) | List Biologicals | Cat#104 |
| Dextran, Fluorescein, 3000MW, Anionic, Lysine Fixable | Invitrogen | Cat#D-3306 |
| Dextran, Tetramethylrhodamine, 3000MW, Anionic, Lysine Fixable | Invitrogen | Cat#D-3308 |
| Experimental Models: Organisms/Strains | ||
| Olig2::cre | Dessaud et al., 2007 | MGI:3774124 |
| FoxP1 conditional | Feng etal., 2010 | N/A |
| PV::FlpO | Madisen et al., 2015 | Jax 022730 |
| Ai65 (RCFL-tdT) | Madisen et al., 2015 | Jax 021875 |
| Ai14 (RCL-tdT) | Madisen et al., 2010 | Jax 007914 |
| ChAT::cre | Rossi et al., 2011 | Jax 006410 |
| Experimental Models: Cell Lines | ||
| HEK293 | Sigma | Cat#85120602–1VL |
| B7GG | Zampieri et al., 2014 | Ed Callaway |
| Oligonucleotides | ||
| FoxP1 Primer 1 5′ CTCCTAGTCACCTTCCCCAGTGC 3′ | Feng et al., 2010 | N/A |
| FoxP1 Primer 2 5′ G AAC ACT GT CG AAT G ACCCTGC 3′ | Feng et al., 2010 | N/A |
| Cre-F 5′ TTGCCGCGCCATCTGCCACCA 3′ | This study | N/A |
| Cre-R 5′ CTAAT CG CC AT CTT CC AG 3′ | This study | N/A |
| PVFlpO 16211 5′ TGTTTCTCCAGCATTTCCAG 3′ | Madisen et al., 2015 | N/A |
| PVFlpO 17564 5′ GGATGCTTGCCGAAGATAAG 3′ | Madisen et al., 2015 | N/A |
| PvFlpO 17566 5′ CTGAGCAGCTACATCAACAGG 3′ | Madisen et al., 2015 | N/A |
| RosaTomWT-F 5′ AAGGGAGCTGCAGTGGAGTA 3′ | Madisen et al., 2010 | N/A |
| RosaTomWT-R 5′ CCGAAAATCTGTGGGAAGTC 3′ | Madisen et al., 2010 | N/A |
| RosaTomMut-F 5′ GGCATTAAAGCAGCGTATCC 3′ | Madisen et al., 2010 | N/A |
| RosaTomMut-R 5′ CTGTTCCTGTACGGCATGG 3′ | Madisen et al., 2010 | N/A |
| Software and Algorithms | ||
| Imaris | Bitplane | N/A |