Comparison of sensitivity of detection of PYMoV by RPA (a, c, e) and PCR (b, d, f) assays. Lane 2–10 loaded with RPA/PCR products obtained with template DNA dilutions from 100–10−8 using template DNA isolated by CTAB (a, b), crude DNA extract (c, d) and cDNA (e, f). Lane M in a, c, d, e are loaded with 100 bp DNA ladder while lane M in b and f are loaded with 1 kb DNA ladder