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. 2020 Mar 19;8:e8802. doi: 10.7717/peerj.8802

Figure 6. The expression of major iron transport machineries in the duodenum of wild type and thalassemic mice.

Figure 6

Quantitative RT-PCR of (A) Cybrd1 (Dcytb) (B) Slc11a2 (DMT1) (C) Slc40a1 (ferroportin) mRNA in the duodenum of WT and BKO mice aged 6–7 weeks and 16–20 weeks. Relative mRNA expression was acquired by normalizing to Actb (β-actin) mRNA. Values are presented as means and SEM for minus delta Ct values (n = 5 per group). Statistical analysis was performed by 2-way ANOVA with Bonferroni post-hoc test. P-values for the effects of phenotype, age and interaction are shown in the figure. Statistical significance for pairwise comparison is indicated by * symbols (*P < 0.05, **P < 0.01, ***P < 0.001).