Table 1. Sequence of gene-specific primers.
Gene product | Forward primer | Reverse primer |
---|---|---|
Actb (β-actin) | CAGCCTTCCTTCTTGGGTA | TTTACGGATGTCAACGTCACAC |
Alas2 (ALAS2) | AGCCATTGTCCTTTCATGCT | CAGCAGGTCTGTCTTGAAAGTCT |
Bmp6 (Bmp6) | GCCAACTACTGTGATGGAGAGTGTT | CTCGGGATTCATAAGGTGGACCA |
Cybrd1 (Dcytb) | TTTGTCCTGAAACACCCCTC | AGAAGGCCCAGCGTATTTGT |
Fam132b (Erfe) | TCCTCTATCTACAGGCAGGAC | ACTGCGTACCGTGAGGGA |
Gdf15 (Gdf15) | GAGCTACGGGGTCGCTTC | GGGACCCCAATCTCACCT |
Hamp (Hepcidin) | CAGGGCAGACATTGCGATAC | GTGGCTCTAGGCTATGTTTTGC |
Hmox1 (HO-1) | CAGAGCCGTCTCGAGCATAG | CAAATCCTGGGGCATGCTGT |
Slc11a2 (DMT1) | TTCTACTTGGGTTGGCAGTGTT | CAGCAGGACTTTCGAGATGC |
Slc25a37 (Mfrn1) | ACGCCATGTATTTTGCCTGC | ACTCCCAGCTACCCCATTAG |
Slc40a1 (ferroportin) | ATCCCCATAGTCTCTGTCAGC | CAGCAACTGTGTCACCGTCA |
Tfrc (TfR1) | TCCTTTCCTTGCATATTCTGG | CCAAATAAGGATAGTCTGCATCC |
Twsg1 (Twsg1) | GCTGTCACACCATGAAAACCTAG | ACTGTGCACATGCGCTCTT |