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. 1994;139(3):417–425. doi: 10.1007/BF01310802

A genetic analysis of macrophage activation and specific antibodies in relation to the resistance of heterogeneous mouse populations to MHV3 infection

R C Vassão 1, O A Sant' Anna 2, C A Pereira 1
PMCID: PMC7086603  PMID: 7832646

Summary

The genetically selected high antibody responder mice (HIII) are susceptible and the low antibody responder mice (LIII) are resistant to the experimental infection with Mouse Hepatitis Virus 3 (MHV3). The mortality rates of the F1 hybrids and of the F2 segregants showed the codominance of the susceptible and resistant characters. The direct individual intrapopulation correlation between the induction of antiviral state in macrophages activated by IFN gamma and the resistance to the virus infection, showed that an antiviral state could be induced in resistant mouse macrophages, whereas in susceptible mouse macrophages no restriction of virus replication could be observed. A direct inter- and intrapopulation correlation of pre-existing antibody titres against MHV3 with the mortality and a direct interpopulation correlation of those titres with the mean survival time of susceptible animals was shown. The data indicate, among the mechanisms of resistance against the virus infection, a role of IFN gamma macrophage-activation and of antibodies against MHV3 which may delay the mean survival time in susceptible animals.

Keywords: Hepatitis, Macrophage Activation, Mouse Macrophage, High Antibody, Direct Inter

References

  • 1.Arnheiter H, Baechi T, Haller O. Adult mouse hepatocytes in primary monolayer culture express genetic resistance to mouse hepatitis virus type 3. J. Immunol. 1982;129:1275–1281. [PubMed] [Google Scholar]
  • 2.Damy SB, Vassao RC, Lucchiari MA, Pereira CA, Sant'Anna OA. A comparative study of resistance to MHV3 infection in genetically homogeneous and heterogeneous mouse populations. Braz J Med Biol Res. 1992;25:1025–1027. [PubMed] [Google Scholar]
  • 3.Dick GNA, Niven JSF, Gledhill AN. A virus related to that causing hepatitis in mice (MHV) Br J Exp Pathol. 1956;37:90–98. [PMC free article] [PubMed] [Google Scholar]
  • 4.Dindzans VJ, Skamene E, Levy GA. Susceptibility/resistance in mouse hepatitis virus strain 3 and macrophage procoagulant activity are linked and controlled by two non-H-2 linked genes. J Immunol. 1986;137:2355–2362. [PubMed] [Google Scholar]
  • 5.Dupuy JM, Dupuy C, Decarie D. Genetically determined resistance to mouse hepatitis virus type 3 is expressed in hematopoietic donor cells in radiation chimeras. J Immunol. 1984;133:1609–1615. [PubMed] [Google Scholar]
  • 6.Hierholzer JC, Broderson JR, Murphy FA. New strain of mouse hepatitis virus as the cause of lethal enteritis in infant mice. Infect Immun. 1984;24:508–522. doi: 10.1128/iai.24.2.508-522.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 7.Le Prevost C, Levy-Leblond E, Virelizier JL, Dupuy JM. Immunopathology of mouse hepatitis virus type 3 infection. I. Role of humoral and cell-mediated immunity in resistance mechanisms. J Immunol. 1975;114:221–227. [PubMed] [Google Scholar]
  • 8.Levy GA, Leibowitz JL, Edgington TS. Induction of monocyte procoagulant activity by murine hepatitis virus type 3 (MHV3) parallels diseases susceptibility in mice. J Exp Med. 1981;154:1150–1157. doi: 10.1084/jem.154.4.1150. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 9.Levy-Leblond E, Oth D, Dupuy JM. Genetic study of mouse sensitivity to MHV3 infection. Influence of the H-2 complex. J Virol. 1979;122:1359–1362. [PubMed] [Google Scholar]
  • 10.Lucchiari MA, Pereira CA. A major role of macrophage activation by interferon gamma during mouse hepatitis virus type 3 infection. I. Genetically dependent resistance. Immunobiology. 1989;180:12–22. doi: 10.1016/S0171-2985(89)80026-9. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 11.Lucchiari MA, Pereira CA. A major role of macrophage activation by interferon gamma during mouse hepatitis virus type 3 infection. II. Age dependent resistance. Immunobiology. 1990;181:31–39. doi: 10.1016/S0171-2985(11)80163-4. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 12.Lucchiari MA, Martin JP, Modollel M, Pereira CA. Acquired immunity of A/J mice to mouse hepatitis virus 3 infection: dependence on interferon gamma synthesis and macrophage sensitivity to interferon gamma. J Gen Virol. 1991;72:1371–1322. doi: 10.1099/0022-1317-72-6-1317. [DOI] [PubMed] [Google Scholar]
  • 13.Lucchiari MA, Modolell M, Eichmann K, Pereira CA. In vivo depletion of interferon gamma leads to susceptibility of A/J mice to mouse hepatitis virus 3 infection. Immunobiology. 1992;185:475–482. doi: 10.1016/S0171-2985(11)80089-6. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 14.Martin JP, Koehren F, Rannou JJ, Kirn A. Temperature sensitive mutants of mouse hepatitis virus type 3 (MHV3): isolation, biochemical and genetic characterization. Arch Virol. 1988;100:147–160. doi: 10.1007/BF01487679. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 15.Mello IGC, Vassao RC, Pereira CA. Virus specificity of the antiviral state induced by IFN gamma correlates with resistance to MHV3 infection. Arch Virol. 1993;132:281–289. doi: 10.1007/BF01309539. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 16.Nilsson MR, Sant'Anna OA, Siqueira M, Nilsson TT, Gennari M. Rabies virus immunity in genetically selected high and low responder lines of mice. Infect Immun. 1979;25:23–26. doi: 10.1128/iai.25.1.23-26.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 17.Pereira CA, Steffan AM, Kirn A. Interaction between mouse hepatitis viruses and primary cultures of Kupffer and endothelial liver cells from resistant and susceptible inbred mouse strains. J Gen Virol. 1984;65:1617–1620. doi: 10.1099/0022-1317-65-9-1617. [DOI] [PubMed] [Google Scholar]
  • 18.Pereira CA, Mercier G, Oth D, Dupuy JM. Induction of natural killer cells and interferon during mouse hepatitis virus infection of resistant and susceptible inbred mouse strains. Immunobiology. 1984;166:35–42. doi: 10.1016/S0171-2985(84)80141-2. [DOI] [PubMed] [Google Scholar]
  • 19.Rowe WP, Hartley JW, Capps WI. Mouse hepatitis virus infection as a highly contagious, prevalent, enteric infection in mice. Proc Soc Exp Biol Med. 1963;112:161–165. doi: 10.3181/00379727-112-27980. [DOI] [PubMed] [Google Scholar]
  • 20.Sant'Anna OA, Massa S, Mouton D, Bouthillier Y, Mevel C, Ibanez OM, Vassao R, De Franco M, Bellinati R, Siqueira M, Biozzi G. Salmonella typhimurium infection in high and low antibody responder mice: inverse correlation between antibody responsiveness and resistance to infection. FEMS Microbiol Immunol. 1989;47:465–472. doi: 10.1111/j.1574-6968.1989.tb02437.x. [DOI] [PubMed] [Google Scholar]
  • 21.Siqueira M, Bandieri A, Reis MH, Sant'Anna OA, Biozzi G. Selective breeding of mice for antibody responsiveness to flagellar and somatic antigens of Salmonellae. Eur J Immunol. 1976;6:241–249. doi: 10.1002/eji.1830060403. [DOI] [PubMed] [Google Scholar]
  • 22.Van der Riet FSJ, Kahn LB. Isolation of a murine hepatitis virus from Swiss mice treated with antilymphocyte serum. Arch Ges Virusforschung. 1973;42:1–8. doi: 10.1007/BF01250502. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 23.Vassao R, Russo M, Macrondes MCG, Pereira CA. Resistance of genetically selected mice to MHV3 infection is not dependent on the H2O2 release by macrophages. Microbiol Pathol. 1993;14:169–176. doi: 10.1006/mpat.1993.1017. [DOI] [PubMed] [Google Scholar]
  • 24.Virelizier JL, Gresser I. Role of interferon in the pathogenesis of viral disease of mice as demonstrated by the use of anti-interferon serum. V. Protective role in mouse hepatitis virus type 3 infection of susceptible and resistant strains of mice. J Immunol. 1978;120:1616–1619. [PubMed] [Google Scholar]

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