Skip to main content
Springer Nature - PMC COVID-19 Collection logoLink to Springer Nature - PMC COVID-19 Collection
. 1980;63(1):75–80. doi: 10.1007/BF01320763

The polypeptides of human and mouse coronaviruses

M R Macnaughton 1
PMCID: PMC7086607  PMID: 6245634

Summary

The polypeptide compositions of two coronaviruses, human coronavirus strain 229E (HCV 229E) and mouse hepatitis virus strain 3 (MHV3), were characterised on polyacrylamide gels. Similar polypeptide patterns were observed for both viruses consisting of large surface projection glycopolypeptides of mol. wt. 160,000 and 105,000 for HCV 229E, and 170,000 and 90,000 for MHV3, two small polypeptides of mol. wt. varying from 24,000 to 20,000, and a polypeptide of mol. wt. 50,000. The results are discussed with respect to previous reports of the polypeptides of these and other coronaviruses.

Keywords: Hepatitis, Infectious Disease, Polypeptide, Polyacrylamide, Hepatitis Virus

Footnotes

With 3 Figures

References

  • 1.Alexander D. J., Collins M. S. The purification and polypeptide composition of avian infectious bronchitis virus. Microbios. 1977;18:87–98. [PubMed] [Google Scholar]
  • 2.Almeida J. D., Berry D. M., Cunningham C. H., Hamre D., Hofstad M. S., Mallucci L., McIntosh K., Tyrrell D. A. J. Coronaviruses. Nature. 1968;220:650. [Google Scholar]
  • 3.Bond C. W., Leibowitz J. L., Robb J. A. Pathogenic murine coronaviruses. II. Characterization of virus-specific proteins of murine coronaviruses JHMV and A59V. Virology. 1979;94:371–384. doi: 10.1016/0042-6822(79)90468-9. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 4.Garwes D. J., Pocock D. H. The polypeptide structure of transmissible gastroenteritis virus. J. gen. Virol. 1975;29:25–34. doi: 10.1099/0022-1317-29-1-25. [DOI] [PubMed] [Google Scholar]
  • 5.Garwes D. J., Pocock D. H., Pike B. V. Isolation of subviral components from transmissible gastroenteritis virus. J. gen. Virol. 1976;32:283–294. doi: 10.1099/0022-1317-32-2-283. [DOI] [PubMed] [Google Scholar]
  • 6.Hierholzer J. C. Purification and biophysical properties of human coronavirus 229E. Virology. 1976;75:155–165. doi: 10.1016/0042-6822(76)90014-3. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 7.Hierholzer J. C., Palmer E. L., Whitfield S. G., Kaye H. S., Dowdle W. R. Protein composition of coronavirus OC43. Virology. 1972;48:516–527. doi: 10.1016/0042-6822(72)90062-1. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 8.Macnaughton M. R., Madge M. H. The polypeptide composition of avian infectious bronchitis virus particles. Arch. Virol. 1977;55:47–54. doi: 10.1007/BF01314478. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 9.Macnaughton M. R., Madge M. H. The genome of human coronavirus strain 229E. J. gen. Virol. 1978;39:497–504. doi: 10.1099/0022-1317-39-3-497. [DOI] [PubMed] [Google Scholar]
  • 10.Macnaughton M. R., Madge M. H., Davies H. A., Dourmashkin R. R. Polypeptides of the surface projections and the ribonucleoprotein of avian infectious bronchitis virus. J. Virol. 1977;24:821–825. doi: 10.1128/jvi.24.3.821-825.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 11.McIntosh K. Coronaviruses: a comparative review. Curr. Top. Microbiol. Immunol. 1974;63:85–129. [Google Scholar]
  • 12.Pocock D. H., Garwes D. J. The polypeptides of haemagglutinating encephalomyelitis virus and isolated subviral particles. J. gen. Virol. 1977;37:487–499. [Google Scholar]
  • 13.Sturman L. S. Characterization of a coronavirus. I. Structural proteins: effects of preparative conditions on the migration of protein in polyacrylamide gels. Virology. 1977;77:637–649. doi: 10.1016/0042-6822(77)90488-3. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 14.Sturman L. S., Holmes K. V. Characterization of a coronavirus: II. Glycoproteins of the viral envelope: tryptic peptide analysis. Virology. 1977;77:650–660. doi: 10.1016/0042-6822(77)90489-5. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 15.Tyrrell D. A. J., Alexander D. J., Almeida J. D., Cunningham C. H., Easterday B. C., Garwes D. J., Hierholzer J. C., Kapikian A., Macnaughton M. R., McIntosh K. Coronaviridae: Second report. Intervirology. 1978;10:321–328. doi: 10.1159/000148996. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 16.Wege H., Wege H., Nagashima K., Ter Meulen V. Structural polypeptides of the murine coronavirus JHM. J. gen. Virol. 1979;42:37–47. doi: 10.1099/0022-1317-42-1-37. [DOI] [PubMed] [Google Scholar]

Articles from Archives of Virology are provided here courtesy of Nature Publishing Group

RESOURCES