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. 1988;100(3):171–183. doi: 10.1007/BF01487681

Mouse hepatitis virus and host determinants of vertical transmission and maternally-derived passive immunity in mice

S W Barthold 1, Deborah S Beck 1, Abigail L Smith 1
PMCID: PMC7086610  PMID: 2840871

Summary

Transmission of mouse hepatitis virus (MHV) in utero following oronasal inoculation of pregnant mice was found to depend upon MHV strain and host genotype. Virulent, polytropic MHV-JHM was recovered from multiple maternal tissues, including liver and uterus, as well as placenta and fetus in susceptible BALB/cByJ mice. Fetuses were infected during all 3 trimesters of pregnancy. Low virulence, polytropic MHV-S infected fetuses in a low percentage of susceptible BALB/cByJ dams. Infection of resistant CD-1 mice with MHV-JHM was limited, with no fetal infection. Enterotropic MHV-Y was largely restricted to intestine of BALB/cByJ and CD-1 dams, with minimal dissemination and no fetal infection. Maternally-derived MHV IgG antibody was detectable in pup sera through 4 weeks of age. Antibody titers were generally lower in second litters of the same dam. Cross-fostering experiments showed that antibody was transferred via colostrum and not in utero, and that pups were capable of absorption through 2 weeks of age. Pups nursing immune dams were protected against MHV challenge at 1 and 2 weeks of age, compared to pups nursing naive dams. Immunity to MHV challenge was cross-protective against both antigenically homotypic and heterotypic strains of MHV.

Keywords: Hepatitis, Antibody Titer, Vertical Transmission, Pregnant Mouse, Passive Immunity

References

  • 1.Barthold SW. Mouse hepatitis virus biology and epizootiology. In: Bhatt PN, Jacoby RO, Morse HC III, New AE, editors. Viral and mycoplasmal infections of laboratory rodents: effects on biomedical research. New York: Academic Press; 1986. pp. 571–601. [Google Scholar]
  • 2.Barthold SW. Host age and genotypic effects on enterotropic mouse hepatitis virus infection. Lab Anim Sci. 1987;37:36–40. [PubMed] [Google Scholar]
  • 3.Barthold SW, Beck DS. Intranasal challenge immunity of mice to antigenically homologous and heterologous strains of mouse hepatitis virus. Adv Exp Med Biol. 1987;218:449–450. doi: 10.1007/978-1-4684-1280-2_55. [DOI] [PubMed] [Google Scholar]
  • 4.Barthold SW, Beck DS, Smith AL. Mouse hepatitis virus nasoencephalopathy is dependent upon virus strain and hot genotype. Arch Virol. 1986;91:247–256. doi: 10.1007/BF01314284. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 5.Barthold SW, Smith AL. Mouse hepatitis virus S in weanling Swiss mice following intranasal inoculation. Lab Anim Sci. 1983;33:355–360. [PubMed] [Google Scholar]
  • 6.Barthold SW, Smith AL. Mouse hepatitis virus strain-related patterns of tissue tropism in suckling mice. Arch Virol. 1984;81:103–112. doi: 10.1007/BF01309300. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 7.Barthold SW, Smith AL. Response of genetically susceptible and resistant mice to intranasal inoculation with mouse hepatitis virus JHM. Virus Res. 1987;7:225–239. doi: 10.1016/0168-1702(87)90030-X. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 8.Barthold SW, Smith AL, Lord PFS, Bhatt PN, Jacoby RO, Main AJ. Epizootic coronaviral typhlocolitis in suckling mice. Lab Anim Sci. 1982;32:376–383. [PubMed] [Google Scholar]
  • 9.Brambell FWR, Hemmings WA. The transmission of antibodies from mother to fetus. In: Villee CA, editor. The placenta and fetal membranes. Baltimore: Williams and Wilkens; 1960. pp. 71–84. [Google Scholar]
  • 10.Galanti B, Balestrieri GG, Piccinino F, Izzo E. Study of possible effect of prednisolone on placental impermeability to MHV-3 virus in pregnant mice. Boll Soc Ital Biol Sper. 1969;45:325–327. [PubMed] [Google Scholar]
  • 11.Hierholzer JC, Broderson JR, Murphy FA. New strain of mouse hepatitis virus as the cause of lethal enteritis in infant mice. Infect Immun. 1979;24:508–522. doi: 10.1128/iai.24.2.508-522.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 12.Hirano N, Murakami T, Taguchi F, Fujiwara K, Matumota M. Comparison of mouse hepatitis virus strains for pathogenicity in weanling mice infected by various routes. Arch Virol. 1981;70:69–73. doi: 10.1007/BF01320795. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 13.Iida T, Tajima M, Murata Y. Transmission of maternal antibodies of Sendai virus in mice and its significance in enzootic infection. J Gen Virol. 1973;18:247–258. doi: 10.1099/0022-1317-18-3-247. [DOI] [PubMed] [Google Scholar]
  • 14.Ishida T, Fujiwara K. Maternally derived immune resistance to fatal diarrhea in infant mice to mouse hepatitis virus. Jpn J Exp Med. 1982;52:231–235. [PubMed] [Google Scholar]
  • 15.Katami K, Taguchi F, Nakayama M, Goto N, Fujiwara K. Vertical transmission of mouse hepatitis virus infection in mice. Jpn J Exp Med. 1978;48:481–490. [PubMed] [Google Scholar]
  • 16.Le Prevost C, Levy-Leblond E, Virelizier J, Dupuy J. Immunopathology of mouse hepatitis virus type 3 infection. I. Role of humoral and cell-mediated immunity in resistance mechanisms. J Immunol. 1975;114:221–225. [PubMed] [Google Scholar]
  • 17.Lipman NS, Newcomer CE, Fox JG. Rederivation of MHV and MEV antibody positive mice by cross-fostering and use of microisolator caging system. Lab Anim Sci. 1987;37:195–199. [PubMed] [Google Scholar]
  • 18.Manaker RA, Piczak CV, Miller AA, Stanton MF. A hepatitis virus complicating studies with mouse leukemia. J Natl Cancer Inst. 1961;27:29–45. [PubMed] [Google Scholar]
  • 19.Perlman S, Schelper R, Bolger E, Ries D. Late onset, symptomatic, demyelinating encephalomyelitis in mice infected with MHV-JHM in the presence of maternal antibody. Microbiol Pathol. 1987;2:185–194. doi: 10.1016/0882-4010(87)90020-9. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 20.Piccinino F, Galanti B, Guisti G. Lack of transplacental transmissibility of MHV-3 virus. Arch Ges Virusforsch. 1966;18:327–332. [Google Scholar]
  • 21.Pickel K, Muller MA, Ter Meulen V. Analysis of age-dependent resistance to murine coronavirus JHM infection in mice. Infect Immun. 1981;34:648–654. doi: 10.1128/iai.34.3.648-654.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 22.Pickel K, Muller MA, Ter Meulen V. Influence of maternal immunity on the outcome of murine coronavirus JHM infection in suckling mice. Med Microbiol Immunol. 1985;174:15–24. doi: 10.1007/BF02123666. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 23.Smith AL, Winograd DF. Two enzyme immunoassays for the detection of antibody to rodent coronaviruses. J Virol Methods. 1986;14:335–343. doi: 10.1016/0166-0934(86)90035-2. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 24.Weir EC, Bhatt PN, Barthold SW, Cameron GA, Simack PA. Elimination of mouse hepatitis virus from a breeding colony by temporary cessation of breeding. Lab Anim Sci. 1987;37:455–458. [PubMed] [Google Scholar]

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