Summary
The influence of pH on the growth of transmissible gastroenteritis virus (TGEV) in adult pig thyroid cell culture, and on the stability of the virus was studied. At pH 6.5 the yield of virus was 10 fold higher than cultures held at pH 7.2 and 100 fold higher than those at pH 8.0. The adsorption, penetration and uncoating steps of the viral replicative cycle were shown to be unaffected by pH variation. Synthesis of TGEV RNA during the first 12 hours post infection was found to be unaffected by pH variation between the range 6.5–8.0. After 12 hours breakdown of this RNA appeared to occur in cultures held at pH 7.2 and 8.0 but not at pH 6.5.
When incubated at 37° C for 24 hours the virus infectivity was found to be least affected by pH 6.5 but when kept at 4° C for the same length of time, the virus infectivity remained constant between pH 5.0 and pH 8.0.
Keywords: Cell Culture, Infectious Disease, Virus Infectivity, Gastroenteritis, Post Infection
Footnotes
With 4 Figures
References
- 1.Cartwright S. F., Harris H. M., Blandford T. B., Fincham I., Gitter M. A cytopathic virus causing transmissible gastroenteritis in swine. J. comp. Pathol. 1965;75:387–396. doi: 10.1016/0021-9975(65)90019-8. [DOI] [PubMed] [Google Scholar]
- 2.Clarke M. C. The effect of 5-bromodeoxyuridine and actinomycin D on the multiplication of transmissible gastroenteritis virus. J. gen. Virol. 1968;3:267–270. doi: 10.1099/0022-1317-3-2-267. [DOI] [PubMed] [Google Scholar]
- 3.Doyle L. P., Hutchings L. M. A transmissible gastroenteritis in pigs. J. Amer. vet. med. Ass. 1946;108:257–259. [PubMed] [Google Scholar]
- 4.Fiszman M., Léauté J.-B., Chany C., Girard M. Mode of action of acid pH values on the development of vesicular stomatitis virus. J. Virol. 1974;13:801–808. doi: 10.1128/jvi.13.4.801-808.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
- 5.Garwes, D. J., Pocock, D. H.: The polypeptide structure of transmissible gastroenteritis virus. J. gen. Virol. (In press.) [DOI] [PubMed]
- 6.Haelterman E. O. Transmissible gastroenteritis of swine. Proc. 17th World Vet. Congr. 1962;1:615–618. [Google Scholar]
- 7.Léauté J.-B., Fiszman M., Chany C., Girard M. Effet de l'influence d'un abaissement de pH sur le métabolisme cellulaire et sur le développement du virus de la poliomyélite. C. R. Acad. Sci. 1971;273:2024–2027. [PubMed] [Google Scholar]
- 8.McClurkin A. W., Norman J. O. Studies on transmissible gastroenteritis of swine. II. Selected characteristics of a cytopathogenic virus common to five isolates from transmissible gastroenteritis. Can. J. comp. Med. 1966;30:190–198. [PMC free article] [PubMed] [Google Scholar]
- 9.Rutter J. M., Luther P. D. Cytopathic factors in bacteria-free lysates of Escherichia coli. J. med. Microbiol. 1973;6:565–571. doi: 10.1099/00222615-6-4-565. [DOI] [PubMed] [Google Scholar]
- 10.Sheffy B. E. Characterisation of transmissible gastroenteritis virus. Proc. 69th Ann. Meet. U.S. Livestock Sanit. Ass. 1965;69:351–363. [Google Scholar]
- 11.Sørenson S. P. L. Ergänzung zu der Abhandlung: Enzymstudien II: Über die Messung und die Bedeutung der Wasserstoffionenkonzentration bei enzymatischen Prozessen. Biochem. Z. 1909;22:352–356. [Google Scholar]
- 12.Tajima M. Morphology of transmissible gastroenteritis virus of pigs. Arch. ges. Virusforsch. 1970;29:105–108. doi: 10.1007/BF01253886. [DOI] [PMC free article] [PubMed] [Google Scholar]
- 13.Witte K. H., Easterday B. C. Isolation and propagation of the virus of transmissible gastroenteritis of pigs in various pig cell cultures. Arch. ges. Virusforsch. 1967;20:327–350. doi: 10.1007/BF01241952. [DOI] [PubMed] [Google Scholar]
- 14.Witte K. H., Easterday B. C. Stained monolayer test: A colour test in disposable plastic trays for titrating transmissible gastroenteritis virus and neutralizing antibodies. Amer. J. vet. Res. 1968;29:1409–1417. [PubMed] [Google Scholar]
- 15.Young G. A., Hinz R. W., Underdahl N. R. Some characteristics of transmissible gastroenteritis in disease-free antibody-devoid pigs. Amer. J. vet. Res. 1955;16:529–535. [PubMed] [Google Scholar]