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. 1981;69(3):219–228. doi: 10.1007/BF01317337

Replication of porcine enteric adenovirusesin vivo

R Ducatelle 1, W Coussement 1, J Hoorens 1
PMCID: PMC7086692  PMID: 6271102

Summary

The replication of an enteropathogenic porcine adenovirus in the intestinal epithelial cells of naturally and experimentally piglets has been studied by transmission electron microscopy (TEM) and by immunoperoxidase (Ip) staining of paraffin sections.

Three types of intranuclear inclusion bodies were observed. Viral particles appeared to be assembled from electron dense crescents which seemed to originate from type II intranuclear inclusion bodies. Virus accumulated in the nucleus of infected cells. It formed paracrystalline arrays in the electron dense centre of the nucleus but was dispersed in the peripheral electron translucent zone. Virus was released from the cells after rupture of the nuclear and plasma membranes.

The results are compared with published data on the replication of adenoviruses in cell cultures.

Keywords: Electron Microscopy, Plasma Membrane, Cell Culture, Epithelial Cell, Transmission Electron Microscopy

Footnotes

With 6 Figures

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