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. 1983;59(4):269–276. doi: 10.1007/BF00691492

Glial proteins in canine distemper virus-induced demyelination

A sequential immunocytochemical study

M Vandevelde 1, P Bichsel 1, S Cerruti-Sola 1, A Steck 2, F Kristensen 3, R J Higgins 4
PMCID: PMC7086711  PMID: 6191513

Summary

A temporal series of demyelinating lesions in experimental canine distemper virus (CDV) infection was examined with immunohistological techniques demonstrating myelin basic protein (MBP), myelin-associated glycoprotein (MAG), and glial fibrillary acidic protein (GFAP) on serial sections. The earliest lesions were characterized by decreased MBP and MAG and increased GFAP. During the further progression of the disease, MBP and MAG losses continued to match each other. There was no indication of MAG loss preceding the disappearance of MBP. In the more advanced lesions there was a marked decrease of GFAP positive cells. Since these findings differed considerably from similar immunohistochemical studies in progressive multifocal leukoencephalopathy (PML) where demyelination results from oligodendroglial infection, it was concluded that the oligodendroglial cell body is not the primary target of CDV. The marked astroglial changes were also considered to contribute to demyelination in CDV infection but the mechanism by which this happens remains unknown.

Key words: Canine distemper, Demyelination, Immunohistology, MBP, MAG, GFAP

Footnotes

Supported by the Swiss National Science Foundation (Grant No. 3.809.81) and the Swiss M. S. Society

References

  1. Appel MJG, Shek WR, Summers BA. Lymphocyte-mediated immune cytotoxicity in dogs infected with virulent canine distemper virus. Infect Immun. 1982;37:592–600. doi: 10.1128/iai.37.2.592-600.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Bradbury MWB. Ontogeny of mammalian brain-barrier systems. In: Cserr HF, Fenstermacher JD, Fencl V, editors. Fluid environment of the brain. New York San Francisco London: Academic Press; 1975. pp. 81–103. [Google Scholar]
  3. Braun PE, Brostoff SW. Proteins of myelin. In: Morell P, editor. Myelin. New York London: Plenum Press; 1977. pp. 201–231. [Google Scholar]
  4. Campbell RSF. Encephalitis in canine distemper. Br Vet J. 1957;113:143–162. [Google Scholar]
  5. Cerruti-Sola S, Kristensen F, Vandevelde M, Bichsel P, Kihm U (1983) Lymphocyte responsiveness to lectin and myelin antigens in canine distemper infection in relation to the development of demyelinating lesions. J Neuroimmunol (in press) [DOI] [PubMed]
  6. Dal Canto MC, Rabinowitz SG. Experimental models of virus-induced demyelination of the central nervous system. Ann Neurol. 1982;11:109–127. doi: 10.1002/ana.410110202. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Fankhauser R. Hundestaupe-Geschichte einer Krankheit. Schweiz Arch Tierheilkd. 1982;124:245–256. [PubMed] [Google Scholar]
  8. Fankhauser R, Luginbühl H. Pathologische Anatomie des zentralen und peripheren Nervensystems der Haustiere. Berlin Hamburg: Parey; 1968. pp. 191–462. [Google Scholar]
  9. Frauchiger E, Fankhauser R. Demyelinating diseases in animals. In: Vinken P, Bruyn GW, editors. Handbook of clinical neurology, vol 9. Amsterdam: North Holland; 1970. [Google Scholar]
  10. Higgins RJ, Krakowa SG, Metzler AE, Koestner A. Primary demyelination in experimental canine distemper virus induced encephalomyelitis in gnotobiotic dogs. Acta Neuropathol (Berl) 1982;58:1–8. doi: 10.1007/BF00692691. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Itoyama Y, Sternberger NH, Webster HdeF, Quarles RH, Cohen SR, Richardson EP. Immunocytochemical observations on the distribution of myelin-associated glycoprotein and myelin basic protein in multiple sclerosis lesions. Ann Neurol. 1980;7:167–177. doi: 10.1002/ana.410070212. [DOI] [PubMed] [Google Scholar]
  12. Itoyama Y, Webster HdeF, Sternberger NH, Richardson EP, Walker DL, Quarles RH, Padgett BL. Distribution of papovavirus, myelin-associated glycoprotein, and myelin basic protein in progressive multifocal leukoencephalopathy lesions. Ann Neurol. 1982;11:396–407. doi: 10.1002/ana.410110414. [DOI] [PubMed] [Google Scholar]
  13. Krakowka S, McCullough B, Koestner A, Olsen R. Myelinspecific autoantibodies associated with central nervous system demyelination in canine distemper virus infection. Infect Immun. 1973;8:819–827. doi: 10.1128/iai.8.5.819-827.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Krakowka S, Higgins RJ, Koestner A. Canine distemper virus: Review of structural and functional modulations in lymphoid tissues. Am J Vet Res. 1980;41:284–292. [PubMed] [Google Scholar]
  15. Lampert PW. Autoimmune and virus-induced demyelinating diseases. Am J Pathol. 1978;91:175–208. [PMC free article] [PubMed] [Google Scholar]
  16. Mászló M, Tariska I. Are astrocytes infected in progressive multifocal leukoencephalopathy (PML)? Acta Neuropathol (Berl) 1982;56:45–51. doi: 10.1007/BF00691181. [DOI] [PubMed] [Google Scholar]
  17. McCullough B, Krakowka S, Koestner A. Experimental canine distemper virus-induced demyelination. Lab Invest. 1974;31:216–222. [PubMed] [Google Scholar]
  18. Nagashima K, Wege H, Meyermann R, ter Meulen V. Corona virus induced subacute demyelinating encephalomyelitis in rats: A morphological analysis. Acta Neuropathol (Berl) 1978;44:63–70. doi: 10.1007/BF00691641. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Peters A, Palay SL, Webster HdeF. The fine structure of the nervous system: The neurons and supporting cells. Philadelphia London Toronto: Saunders; 1976. [Google Scholar]
  20. Raine CS. On the development of CNS lesions in natural canine distemper. Neurol Sci. 1976;30:13–28. doi: 10.1016/0022-510x(76)90251-3. [DOI] [PubMed] [Google Scholar]
  21. Raine CS. Morphological aspects of myelin and myelination. In: Morell P, editor. Myelin. New York London: Plenum Press; 1977. pp. 1–89. [Google Scholar]
  22. Raine CS, Scheinberg L, Waltz JM. Multiple sclerosis—Oligodendrocyte survival and proliferation in an active established lesion. Lab Invest. 1981;45:534–546. [PubMed] [Google Scholar]
  23. Saunders LZ. Some historical aspects of the neuropathology of canine distemper. Schweiz Arch Neurol Psychiatr. 1973;112:341–352. [PubMed] [Google Scholar]
  24. Sofroniew MV, Schrell U. Long-term storage and regular repeated use of diluted antisera in glass staining jars of increased sensitivity, reproducibility, and convenience of single- and two-color light-microscopic immunocytochemistry. J Histochem Cytochem. 1982;30:504–511. doi: 10.1177/30.6.6178778. [DOI] [PubMed] [Google Scholar]
  25. Spielmeyer W. Histopathologie des Nervensystems. Berlin: Springer; 1922. [Google Scholar]
  26. Sternberger LA. Immunocytochemistry. 2nd edn. New York Chichester Brisbane Toronto: Wiley & Sons; 1979. pp. 122–129. [Google Scholar]
  27. Sternberger NH, Quarles RH, Itoyama Y, Webster HdeF. Myelin-associated glycoprotein demonstrated immunocyto-chemically in myelin and myelin-forming cells of developing rat. Neurobiology. 1979;76:1510–1514. doi: 10.1073/pnas.76.3.1510. [DOI] [PMC free article] [PubMed] [Google Scholar]
  28. Summers BA, Greisen HA, Appel MGJ. Early events in canine distemper demyelinating encephalomyelitis. Acta Neuropathol (Berl) 1979;46:1–10. doi: 10.1007/BF00684797. [DOI] [PubMed] [Google Scholar]
  29. Townsend JJ. The relationship of astrocytes and macrophages to CNS demyelination after experimental Herpes simplex virus infection. J Neuropathol Exp Neurol. 1981;40:369–379. doi: 10.1097/00005072-198107000-00002. [DOI] [PubMed] [Google Scholar]
  30. Vandevelde M, Kristensen B. Observations on the distribution of canine distemper virus in the central nervous system of dogs with demyelinating encephalitis. Acta Neuropathol (Berl) 1977;40:233–236. doi: 10.1007/BF00691959. [DOI] [PubMed] [Google Scholar]
  31. Vandevelde M, Kristensen F, Kristensen B, Steck AJ, Kihm U. Immunological and pathological findings in demyelinating encephalitis associated with canine distemper virus infection. Acta Neuropathol (Berl) 1982;56:1–8. doi: 10.1007/BF00691174. [DOI] [PubMed] [Google Scholar]
  32. Vandevelde M, Higgins RJ, Kristensen B, Kristensen F, Steck AJ, Kihm U. Demyelination in experimental canine distemper virus infection: Immunological, pathologic, and immunohistological studies. Acta Neuropathol (Berl) 1982;56:285–293. doi: 10.1007/BF00691260. [DOI] [PubMed] [Google Scholar]
  33. Wisniewski HM, Raine CS, Kay WJ. Observations on viral demyelinating encephalomyelitis. Canine distemper. Lab Invest. 1972;26:589–599. [PubMed] [Google Scholar]

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