Summary
Minimal-disease (MD) cats raised in a barrier-maintained feline breeding colony routinely become coronavirus (CV) antibody-positive at 5–8 weeks of age and remain seropositive indefinitely. In addition, they shed coronavirus-like particles (CVLPs) in their feces which are morphologically distinct from “typical” coronaviruses. Oronasal, intraperitoneal, intraduodenal, and intravenous inoculations of CVLPs were uniformly unsuccessful in the seroconversion of cats to CV, whereas subsequent prolonged close contact with colony cats did result in seroconversion. Such evidence indicates that MD cats in this barrier colony are infected with at least two non-cross-reactive agents: (1) feline enteric CVLPs, which are shed in feces and are morphologically and antigenically distinct from established coronaviruses (CVs), and (2) a virus which is antigenically cross-reactive with CVs, but which is apparently shed by some route other than the lower gastrointestinal tract.
Keywords: Experimental Study, Infectious Disease, Gastrointestinal Tract, Close Contact, Breeding Colony
Footnotes
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References
- 1.Barlough J. E., Jacobson R. H., Downing D. R., Marcella K. L., Lynch T. J., Scott F. W. Evaluation of a computer-assisted, kinetics-based enzyme-linked immuno sorbent assay for detection of coronaviral antibodies in cats. J. clin. Microbiol. 1983;17:202–217. doi: 10.1128/jcm.17.2.202-217.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
- 2.Bohl E. H. Coronaviruses: Diagnosis of infections. In: Kurstak E., Kurstak C., editors. Comparative Diagnosis of Viral Diseases, Vol. IV, Part B. New York: Academic Press; 1981. pp. 301–328. [Google Scholar]
- 3.Caul E. O., Egglestone S. I. Further studies on human enteric coronaviruses. Arch. Virol. 1977;54:107–117. doi: 10.1007/BF01314383. [DOI] [PMC free article] [PubMed] [Google Scholar]
- 4.Caul E. O., Egglestone S. I. Coronavirus-like particles present in simian faeces. Vet. Rec. 1979;104:168–169. doi: 10.1136/vr.104.8.168. [DOI] [PubMed] [Google Scholar]
- 5.Dea S., Roy R. S., Elazhary M. A. S. Y. Coronavirus-like particles in the feces of a cat with diarrhea. Can. vet. J. 1982;23:153–155. [PMC free article] [PubMed] [Google Scholar]
- 6.Eugster A. K., Liauw H. Detection of antibodies to feline infectious peritonitis (FIP) virus in cats using transmissible gastroenteritis virus as antigen and an electronmicroscopic search for FIP virus. Southwestern Vet. 1979;32:109–112. [Google Scholar]
- 7.Hayashi T., Watabe Y., Nakayama H., Fujiwara K. Enteritis due to feline infectious virus peritonitis. Jpn. J. vet. Sci. 1982;44:97–106. doi: 10.1292/jvms1939.44.97. [DOI] [PubMed] [Google Scholar]
- 8.Horzinek M. C., Lutz H., Pedersen N. C. Antigenic relationships among homologous structural polypeptides of porcine, feline, and canine coronaviruses. Infect. Immun. 1982;37:1148–1155. doi: 10.1128/iai.37.3.1148-1155.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
- 9.Horzinek M. C., Osterhaus A. D. M. E., Ellens D. J. Feline infectious peritonitis virus. Zbl. Vet. Med. B. 1977;24:398–405. doi: 10.1111/j.1439-0450.1977.tb01013.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
- 10.Hoshino, Y., Baldwin, C. A., Scott, F. W.: New insights in gastrointenstinal viruses. Cor. Fel. Health Ctr. News, No. 2, 2–4 (1981).
- 11.Hoshino Y., Scott F. W. Coronavirus-like particles in the feces of normal cats. Arch. Virol. 1980;63:147–152. doi: 10.1007/BF01320772. [DOI] [PMC free article] [PubMed] [Google Scholar]
- 12.Howatson A. F. Electron microscopic procedures in virology. In: Habel K., Salzman N. P., editors. Fundamental Techniques in Virology. New York: Academic Press; 1969. pp. 505–524. [Google Scholar]
- 13.MacNaughton M. R., Davies H. A. Human enteric coronaviruses. Arch. Virol. 1981;70:301–313. doi: 10.1007/BF01320245. [DOI] [PMC free article] [PubMed] [Google Scholar]
- 14.Pedersen N. C., Boyle J. F., Floyd K., Fudge A., Barker J. An enteric coronavirus infection of cats and its relationship to feline infectious peritonitis. Am. J. vet. Res. 1981;42:368–377. [PubMed] [Google Scholar]
- 15.Pedersen N. C., Ward J., Mengeling W. L. Antigenic relationship of the feline infectious peritonitis virus to coronaviruses of other species. Arch. Virol. 1978;58:45–53. doi: 10.1007/BF01315534. [DOI] [PMC free article] [PubMed] [Google Scholar]
- 16.Pocock D. H. Effect of sulfhydryl reagents on the biological activities, polypeptide composition and morphology of haemagglutinating encephalomyelitis virus. J. gen. Virol. 1978;40:93–101. doi: 10.1099/0022-1317-40-1-93. [DOI] [PubMed] [Google Scholar]
- 17.Reynolds D. J., Garwes D. J., Lucey S. Differentiation of canine coronavirus and porcine transmissible gastroenteritis virus by neutralisation with canine, porcine, and feline sera. Vet. Microbiol. 1980;5:283–290. [Google Scholar]
- 18.Tyrrell D. A. J., Alexander D. J., Almeida J. D., Cunningham C. H., Easterday B. C., Garwes D. J., Hierholzer J. C., Kapikian A., Macnaughton M. R., McIntosh K. Coronaviridae: Second report. Intervirol. 1978;10:321–328. doi: 10.1159/000148996. [DOI] [PMC free article] [PubMed] [Google Scholar]
- 19.Wirahadiredja R. M. S., Anakotta J., Osterhaus A. D. M. E. Detection by immunofluorescence of transmissible gastroenteritis (TGE) viral antigen in pigs, using cat anti-feline infectious peritonitis (FIP) virus conjugate. Zbl. Vet. Med. B. 1978;25:775–778. doi: 10.1111/j.1439-0450.1978.tb01072.x. [DOI] [PubMed] [Google Scholar]
- 20.Witte K. H., Tuch K., Dubenkropp H., Walther C. Untersuchungen über die Antigenverwandtschaft der Viren der Felinen Infektiösen Peritonitis (FIP) und der Transmissiblen Gastroenteritis (TGE) des Schweines. Berl. Münch. tierärztl. Wschr. 1977;90:396–401. [PubMed] [Google Scholar]