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. 1978;166(1):157–163. doi: 10.1007/BF02121145

Comparison of five diagnostic methods for the detection of rotavirus antigens in calf faeces

D J Ellens 1,✉, P W de Leeuw 1, P J Straver 1, J A M van Balken 1
PMCID: PMC7086822  PMID: 214679

Abstract

Immunoelectroosmophoresis, complement fixation, immunofluorescence on inoculated cell cultures, electron microscopy, and an enzyme-linked immunosorbent assay were compared for the detection of rotavirus in faecal samples from calves. Rotavirus particles could be detected in 39 out of 98 faecal samples by electron microscopy. Immunofluorescence, complement fixation, and immunoelectroosmophoresis detected rotavirus antigens in 39, 42, and 30 samples, respectively. The enzyme-linked immunosorbent assay demonstrated rotavirus antigens in 49 faecal samples. The assay is not only sensitive but also simple to perform and suitable for large-scale testing.

Keywords: Electron Microscopy, Cell Culture, Immunosorbent Assay, Faecal Sample, Diagnostic Method

References

  • 1.Avrameas S., Ternynck T. Peroxidase labeled antibody and Fab conjugates with enhanced intracellular penetration. Immunochemistry. 1971;8:1175–1179. doi: 10.1016/0019-2791(71)90395-8. [DOI] [PubMed] [Google Scholar]
  • 2.Bridger J.C., Woode G.N. Neonatal calf diarrhoea: identification of a reovirus-like (rotavirus) agent in faeces by immunofluorescence and immune electron microscopy. Br. Vet. J. 1975;131:528–535. [PubMed] [Google Scholar]
  • 3.Ellens D.J., de Leeuw P.W. Detection of infantile gastroenteritis virus (rotavirus) by ELISA. Lancet. 1977;i:1363. doi: 10.1016/s0140-6736(77)92574-0. [DOI] [PubMed] [Google Scholar]
  • 4.Ellens D.J., de Leeuw P.W. An enzyme-linked immunosorbent assay (ELISA) for the diagnosis of rotavirus infections in calves. J. Clin. Microbiol. 1977;6:530–532. doi: 10.1128/jcm.6.5.530-532.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 5.Grauballe P.C., Grenner J., Meyling A., Hornsleth A. Rapid diagnosis of rotavirus infections: Comparison of electron microscopy and immunoelectroosmophoresis for the detection of rotavirus in human infantile gastroenteritis. J. Gen. Virol. 1977;35:203–218. doi: 10.1099/0022-1317-35-2-203. [DOI] [PubMed] [Google Scholar]
  • 6.Kalica A.R., Purcell R.H., Sereno M.M., Wyatt R.G., Kim H.W., Chanock R.M., Kapikian A.Z. A microtiter solid phase radioimmunoassay for the detection of the human reovirus-like agent in stools. J. Immun. 1977;118:1275–1279. [PubMed] [Google Scholar]
  • 7.de Leeuw P.W., van Nieuwstadt A.P.K.M.I., van Balken J.A.M., Ellens D.J. Rotavirus infections in calves II. Experimental infections with a Dutch isolate. Neth. J. Vet. Sci. 1977;102:515–524. [PubMed] [Google Scholar]
  • 8.Mebus C.A., Underdahl N.R., Stair E.L., Twiehaus M.J. Neonatal calf diarrhea of a viral etiology; Philadelphia, Pennsylvania: Am. Ass. of Bovine Practitioners; 1970. p. 442. [Google Scholar]
  • 9.Middleton P.J., Petric M., Hewitt C.M., Szymanski M.T., Tam J.S. Counter-immuno-electro-osmophoresis for the detection of infantile gastroenteritis virus antigen and antibody. J. Clin. Pathol. 1976;29:191–197. doi: 10.1136/jcp.29.3.191. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 10.Middleton P.J., Holdaway M.D., Petric M., Szymanski M.T., Tam J.S. Solid-phase radioimmunoassay for the detection of rotavirus. Infect. Immun. 1977;16:439–444. doi: 10.1128/iai.16.2.439-444.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 11.Peterson M., Spendlove R., Smatt R. Detection of neonatal calf diarrhea virus, infant reovirus-like diarrhea virus, and a coronavirus using the fluorescent virus precipitin test. J. Clin. Microbiol. 1976;3:376–377. doi: 10.1128/jcm.3.3.376-377.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 12.Saif L.J., Bohl E.H., Kohler E.M., Hughes J.H. Immune electron microscopy of transmissible gastroenteritis virus and rotavirus (reovirus-like agent) of swine. Am. J. Vet. Res. 1977;38:13–20. [PubMed] [Google Scholar]
  • 13.Scherrer R., Bernard S. Application d'une technique immunoenzymologique (ELISA) à la détection du rotavirus bovin et des anticorps dirigés contre lui. Ann. Microbiol. (Inst. Pasteur) 1977;128 A:499–510. [PubMed] [Google Scholar]
  • 14.Spence L., Fauvel M., Bouchard S., Babiuk L., Saunders J.R. Test for reovirus-like agent. Lancet. 1975;ii:322. doi: 10.1016/s0140-6736(75)92752-x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 15.Tufvesson B., Johnsson T. Immunoelectroosmophoresis for detection of reolike virus: methodology and comparison with electron microscopy. Acta Path. Microbiol. Scand. Sect. B. 1976;84:225–228. doi: 10.1111/j.1699-0463.1976.tb01929.x. [DOI] [PubMed] [Google Scholar]
  • 16.Voller A., Bartlett A., Bidwell D.E., Clark M.F., Adams A.N. The detection of viruses by enzyme-linked immunosorbent assay (ELISA) J. Gen. Virol. 1976;33:165–167. doi: 10.1099/0022-1317-33-1-165. [DOI] [PubMed] [Google Scholar]
  • 17.Wolters G., Kuijpers L., Kacaki J., Schuurs A. Solid-phase enzyme-immunoassay for detection of hepatitis B surface antigen. J. Clin. Pathol. 1976;29:873–879. doi: 10.1136/jcp.29.10.873. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 18.Woode G.N., Bridger J.C. Viral enteritis of calves. Vet. Rec. 1975;96:85–88. doi: 10.1136/vr.96.4.85. [DOI] [PubMed] [Google Scholar]
  • 19.Yolken R.H., Kim H.W., Clem J., Wyatt R.G., Kalica A.R., Chanock R.M., Kapikian A.Z. Enzyme-linked immunosorbent assay (ELISA) for detection of human reovirus-like agent of infantile gastroenteritis. Lancet. 1977;ii:263–267. doi: 10.1016/s0140-6736(77)90951-5. [DOI] [PubMed] [Google Scholar]

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