Summary
Coronavirus-like particles were detected by electron microscopy in the intestinal contents of pigs during a diarrheal outbreak on 4 swine breeding farms. Diarrhea was reproduced in experimental pigs with one of the isolates, designated CV777, which was found to be distinct from the 2 known porcine coronaviruses, transmissible gastroenteritis virus and hemagglutinating encephalomyelitis virus.
Keywords: Electron Microscopy, Infectious Disease, Diarrhea, Gastroenteritis, Encephalomyelitis
Footnotes
With 2 Figures
These studies were supported by the Institute for the Encouragement of Research in Industry and Agriculture (IWONL), Brussels, Belgium.
References
- 1.Cartwright S. F., Lucas M., Cavill J. P., Gush A. F., Blandford T. B. Vomiting and wasting disease of piglets. Vet. Rec. 1969;84:175–176. doi: 10.1136/vr.84.7.175. [DOI] [PubMed] [Google Scholar]
- 2.Doyle L. P., Hutchings L. M. A transmissible gastroenteritis in pigs. J. Amer. vet. Assoc. 1946;108:257–259. [PubMed] [Google Scholar]
- 3.Flewett T. H., Boxall E. The hunt for viruses in infections of the alimentary system: an immuno electronmicroscopical approach. Clinics in Gastroenterology. 1976;5:359–385. [PubMed] [Google Scholar]
- 4.Greig A. S., Mitchell D., Corner A. H., Bannister G. L., Meads E. B., Julian R. J. A hemagglutinating virus producing encephalomyelitis in baby pigs. Canad. J. comp. Med. vet. Sci. 1962;26:49–56. [PMC free article] [PubMed] [Google Scholar]
- 5.Mathan M., Mathan V. I., Swaminathan S. P., Yesudoss S., Baker S. J. Pleomorphic virus-like particles in human faeces. Lancet. 1975;1:1068–1069. doi: 10.1016/S0140-6736(75)91832-2. [DOI] [PMC free article] [PubMed] [Google Scholar]
- 6.McNulty M. S., Curran W. L., McFerran J. B. Virus-like particles in calves' faeces. Lancet. 1975;2:78–79. doi: 10.1016/S0140-6736(75)90524-3. [DOI] [PMC free article] [PubMed] [Google Scholar]
- 7.Pensaert M. B., Haelterman E. O., Burnstein T. Diagnosis of transmissible gastroenteritis in pigs by immunofluorescence. Canad. J. comp. Med. 1968;32:555–561. [PMC free article] [PubMed] [Google Scholar]
- 8.Pensaert M. B., Callebaut P. E. Characteristics of a coronavirus causing vomition and wasting in pigs. Arch. ges. Virusforsch. 1974;44:35–50. doi: 10.1007/BF01242179. [DOI] [PMC free article] [PubMed] [Google Scholar]
- 9.Pensaert, M., de Bouck, P., de Roose, P.: A virus isolated from an apparently new epizootic diarrhea in swine. Proc. 5th World Int. Pig Vet. Congress, Zagreb, Yugoslavia, June 1978, Abstract K. A. 9.
- 10.Philip J. I. H., Cartwright S. F., Scott A. C. The size and morphology of transmissible gastroenteritis and vomiting and wasting disease viruses of pigs. Vet. Rec. 1971;88:311–312. doi: 10.1136/vr.88.12.311. [DOI] [PubMed] [Google Scholar]
- 11.Pirtle E. C. Titration of two porcine respiratory viruses in mammalian cell cultures by direct fluorescent antibody staining. Amer. J. vet. Res. 1974;35:249–250. [PubMed] [Google Scholar]
- 12.Witte K. H. Isolation of the virus of transmissible gastroenteritis (TGE) from naturally infected piglets in cell culture. Zbl. Vet. Med. B. 1971;18:770. doi: 10.1111/j.1439-0450.1971.tb01654.x. [DOI] [PubMed] [Google Scholar]
- 13.Woode G. N. Transmissible gastroenteritis of swine. The Vet. Bull. 1969;39:239. [Google Scholar]
- 14.Woode G. N., Bridger J., Hall G. A., Jones J. M., Jackson G. The isolation of reovirus-like agents (rotaviruses) from acute gastroenteritis of piglets. J. med. Microbiol. 1976;9:203–209. doi: 10.1099/00222615-9-2-203. [DOI] [PubMed] [Google Scholar]