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. 1993;182(5):271–280. doi: 10.1007/BF00579625

Analysis of virus-specific RNA species and proteins in Freon-113 preparations of the Borna disease virus

J A Rieht 1, J E Clements 2, S Herzog 1, J Pyper 2, K Wahn 1, H Becht 1
PMCID: PMC7086879  PMID: 8283962

Abstract

Treatment of homogenates from Borna disease virus (BDV)-infected brain tissue or cell cultures with Freon-113 yielded infectious particles with a buoyant density of 1.16–1.22 g/ml. Positive- and negative-stranded BDV-specific RNA species as well as three virus-specific proteins, known to be present in BDV-infected cell extracts, were demonstrated in these Freon-treated fractions. When the Freon-purified virus preparations were treated with RNase A prior to RNA extraction, only negative-stranded, genomic RNA was detected in Northern blot hybridizations using sense and antisense RNA probes. These data substantiate that BDV is a negative-stranded RNA virus.

Keywords: Northern Blot, Blot Hybridization, Buoyant Density, Northern Blot Hybridization, Virus Preparation

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