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. 1979;60(3):217–226. doi: 10.1007/BF01317493

Pathogenesis of diarrhoea caused by astrovirus infections in lambs

D R Snodgrass 1, K W Angus 1, E W Gray 1, J D Menzies 1, G Paul 2
PMCID: PMC7086881  PMID: 116623

Summary

Experimental infection of 2-day-old gnotobiotic lambs with lamb astrovirus produced mild diarrhoea after an incubation period of about 48 hours. No other clinical symptoms developed. Infection was studied by immunofluorescent and histological examination of tissues from the lambs. Astroviruses infected only mature villus epithelial cells and subepithelial macrophages in the small intestine, where they produced partial villus atrophy. Infected enterocytes were replaced with cuboidal cells from the crypts, and the lesion gradually healed by 5 days after infection. No serological relationship was detected by immunofluorescence between lamb astrovirus antigen in gut sections and antisera to either calf or human astrovirus.

Keywords: Epithelial Cell, Infectious Disease, Small Intestine, Clinical Symptom, Incubation Period

Footnotes

With 6 Figures

References

  • 1.Dahlqvist A. Method for assay of intestinal disaccharidases. Anal. Biochem. 1964;7:18–25. doi: 10.1016/0003-2697(64)90115-0. [DOI] [PubMed] [Google Scholar]
  • 2.Hall G. A., Bridger J. C., Chandler R. L., Woode G. N. Gnotobiotic piglets experimentally infected with neonatal calf diarrhoea reovirus-like agent (rotavirus) Vet. Pathol. 1976;13:197–210. doi: 10.1177/030098587601300304. [DOI] [PubMed] [Google Scholar]
  • 3.Keenan K. P., Jevis H. R., Marchwicki R. H., Binn L. N. Intestinal infection of neonatal dogs with canine coronavirus 1–71: studies by virologic, histologic histochemical and immunofluorescent techniques. Amer. J. vet. Res. 1976;37:247–256. [PubMed] [Google Scholar]
  • 4.Kurtz J. B., Lee T. W., Pickering D. Astrovirus associated gastroenteritis in a children's ward. J. clin. Pathol. 1977;30:948–952. doi: 10.1136/jcp.30.10.948. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 5.Kurtz, J. B., Lee, T. W., Craig, J. W., Reed, S. E.: Astrovirus infection in volunteers. J. med. Virol. (in press). [DOI] [PubMed]
  • 6.Madeley C. R., Cosgrove B. P. 28 nm particles in faeces in infantile gastroenteritis. Lancet. 1975;ii:451–452. doi: 10.1016/s0140-6736(75)90858-2. [DOI] [PubMed] [Google Scholar]
  • 7.Madeley C. R., Cosgrove B. P., Bell E. J., Fallon R. J. Stool viruses in babies in Glasgow. J. Hyg. 1977;78:261–273. doi: 10.1017/s0022172400056151. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 8.Mebus C. A., Stair E. L., Underdahl N. R., Twiehaus M. J. Pathology of neonatal calf diarrhoea induced by a reolike virus. Vet. Pathol. 1971;8:490–505. [Google Scholar]
  • 9.Mebus C. A., Newman L. E., Stair E. L. Scanning electron, light and transmission electron microscopy of intestine of gnotobiotic calf. Amer. J. vet. Res. 1975;36:985–993. [PubMed] [Google Scholar]
  • 10.Mebus C. A., Newman L. E., Stair E. L. Scanning electron, light and immunofluorescent microscopy of intestine of gnotobiotic calf infected with calf diarrheal coronavirus. Amer. J. vet. Res. 1975;36:1719–1725. [PubMed] [Google Scholar]
  • 11.Moon H. W. Vacuolated villous epithelium of the small intestine of young pigs. Vet. Pathol. 1972;9:3–21. doi: 10.1177/030098587200900102. [DOI] [PubMed] [Google Scholar]
  • 12.Pearson G. R., McNulty M. S. Pathological changes in the small intestine of neonatal pigs infected with a pig reovirus-like agent (rotavirus) J. comp. Pathol. 1977;87:363–375. doi: 10.1016/0021-9975(77)90026-3. [DOI] [PubMed] [Google Scholar]
  • 13.Pensaert M., Haelteman E. O., Burnstein T. Transmissible gastroenteritis of swine: Virus-intestinal cell interactions. Arch. ges. Virusforsch. 1970;31:321–334. doi: 10.1007/BF01253767. [DOI] [PubMed] [Google Scholar]
  • 14.Schnagl R. D., Holmes I. H., Mackay-Scollay E. M. A survey of rotavirus associated with gastroenteritis in Aboriginal children in Western Australia. Med. J. Aust. 1978;i:304–307. doi: 10.5694/j.1326-5377.1978.tb107863.x. [DOI] [PubMed] [Google Scholar]
  • 15.Snodgrass D. R., Gray E. W. Detection and transmission of 30 nm virus particles (astroviruses) in faeces of lambs with darrhoea. Arch. Virol. 1977;55:287–291. doi: 10.1007/BF01315050. [DOI] [PubMed] [Google Scholar]
  • 16.Snodgrass D. R., Angus K. W., Gray E. W. Rotavirus infection in lambs: pathogenesis and pathology. Arch. Virol. 1977;55:263–274. doi: 10.1007/BF01315048. [DOI] [PubMed] [Google Scholar]
  • 17.Snodgrass D. R., Herring J. A., Gray E. W. Experimental rotavirus infection in lambs. J. comp. Pathol. 1976;86:637–642. doi: 10.1016/0021-9975(76)90073-6. [DOI] [PubMed] [Google Scholar]
  • 18.Theil K. W., Bohl E. H., Cross R. F., Kohler E. M., Agnes A. G. Pathogenesis of porcine rotaviral infection in experimentally inoculated gnotobiotic pigs. Amer. J. vet. Res. 1978;39:213–220. [PubMed] [Google Scholar]
  • 19.Woode G. N., Bridger J. C. Isolation of small viruses, astrovirus-like and calicivirus-like, from acute enteritis of calves. J. med. Microbiol. 1978;11:441–452. doi: 10.1099/00222615-11-4-441. [DOI] [PubMed] [Google Scholar]

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