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. 1975;161(2):123–126. doi: 10.1007/BF02121753

Virus isolation and titration at 33‡ and 37‡ C

N Fuchs 1, R Wigand 1
PMCID: PMC7086892  PMID: 167268

Abstract

Various prototype viruses and original specimens were comparatively titrated in cell cultures at 33‡ and 37‡ C. Higher titers at 37‡ were consistently obtained with adenoviruses; for other viruses (enteroviruses, herpesvirus hominis, vaccinia virus, parainfluenza viruses) the titers were mostly identical at either temperature. Original specimens and prototype strains showed the same behavior. The habit to cultivate viruses from throat swabs at 33‡ C is unsatisfactory for adenoviruses.

Keywords: Cell Culture, High Titer, Vaccinia Virus, Virus Isolation, Vaccinia

Footnotes

Aided by a grant of the “Bundesministerium für Jugend, Familie und Gesundheit”, Federal Republic of Germany.

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