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. 1981;69(2):117–129. doi: 10.1007/BF01315155

Establishment and maintenance of a persistent infection of L132 cells by human coronavirus strain 229E

Gillian Chaloner-Larsson 1, C Margaret Johnson-Lussenburg 1
PMCID: PMC7086901  PMID: 6171237

Summary

A persistent infection by human coronavirus 229E (HCV/229E) was established in a human continuous cell line (L132). Following the initial infection with stock HCV/229E, several cultures were established of which two (HV1 and HV4) have been maintained by continuous passage for two years. These cultures have shed high titres of infectious virus continuously into the supernatant fluid since their initiation. The persistently infected cells were resistant to homologous super-infection but supported polio virus replication to normal titres. Preliminary tests indicated that 50–100 percent of the cells contain virus. Neither interferon nor reverse transcriptase could be detected in these cultures and the presence of defective interfering particles could not be demonstrated. VH1 and VH4 coronaviruses, isolated from these persistently infected cultures (HV) and identified by 229E antiserum neutralization, were more cytocidal than the parent virus as judged by plaque characteristics and CPE however, they were indistinguishable on the basis of density, EM morphology, and genome size. Present evidence indicates that temperature plays an important but as yet undetermined role in the establishment and maintenance of stable 229E persistently infected cell cultures.

Keywords: Infected Cell, L132 Cell, Persistent Infection, Infectious Virus, Supernatant Fluid

Footnotes

With 5 Figures

References

  • 1.Apolostov K., Spasic P., Bojanic N. Comparative ultrastructural studies on endemic (Balkan) nephropathy and chicken embryo nephritis caused by infectious bronchitis virus. I. Endemic (Balkan) nephropathy. Acta Med. Jug. 1977;31:189–204. [PubMed] [Google Scholar]
  • 2.Armen R. C., Evermann J. F., Truant A. L., Laughlin C. A., Hallum J. V. Temperature sensitive mutants of measles virus produced from persistently infected HeLa cells. Arch. Virol. 1977;53:121–132. doi: 10.1007/BF01314853. [DOI] [PubMed] [Google Scholar]
  • 3.Burks J. S., DeVald B. L., Janovsky L. D., Gerdes J. C. Two coronaviruses isolated from central nervous system tissue of two multiple sclerosis patients. Science. 1980;209:933–934. doi: 10.1126/science.7403860. [DOI] [PubMed] [Google Scholar]
  • 4.Friedman R. M., Ramseur J. M. Mechanisms of persistent infections by cytopathic viruses in tissue culture. Arch. Virol. 1979;60:83–103. doi: 10.1007/BF01348025. [DOI] [PubMed] [Google Scholar]
  • 5.Herndon R. M., Price D., Weiner L. P. Mouse hepatitis virus-induced recurrent demyelination. Arch. Neurol. 1975;32:32–35. doi: 10.1001/archneur.1975.00490430054008. [DOI] [PubMed] [Google Scholar]
  • 6.Holland J. J., Villarreal L. P., Welsh R. M., Oldstone M. B. A., Kohne D., Lazzarini R., Scolnick E. Long-term persistent vesicular stomatitis virus and rabies virus infection of cellsin vitro. J. gen. Virol. 1976;33:193–211. doi: 10.1099/0022-1317-33-2-193. [DOI] [PubMed] [Google Scholar]
  • 7.Huang A. S., Baltimore D. Defective interfering animal viruses. In: Fraenkel-Conrat H., Wagner R. R., editors. Comprehensive Virology, Vol. 10. New York: Plenum Press; 1977. pp. 73–116. [Google Scholar]
  • 8.Kennedy D. A., Johnson-Lussenburg C. M. Isolation and morphology of the internal component of human coronavirus strain 229 E. Intervirol. 1977;6:197–206. doi: 10.1159/000149474. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 9.Lucas A., Coulter M., Anderson R., Dales S., Flintoff W. In vivo andin vitro models of demyelinating diseases. II. Persistence and host-regulated thermosensitivity in cells of neural derivation infected with hepatitis and measles viruses. Virol. 1978;88:325–337. doi: 10.1016/0042-6822(78)90289-1. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 10.Lucas A., Flintoff W., Anderson R., Percy D., Coulter M., Dales S. In vivo andin vitro models of demyelinating diseases: Tropism of the JHM strain of murine hepatitis virus for cells of glial origin. Cell. 1977;12:553–560. doi: 10.1016/0092-8674(77)90131-3. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 11.Monto A. S. Coronaviruses. In: Evans A. S., editor. Viral infections of humans. New York: Plenum Press; 1976. pp. 127–141. [Google Scholar]
  • 12.Moore B., Lee P., Hewish M., Dixon B., Mukherjee T. Coronaviruses in training centre for intellectually retarded. Lancet. 1977;1:261. doi: 10.1016/S0140-6736(77)91064-9. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 13.Nishiyama Y. Studies of L cells persistently infected with VSV: Factors involved in the regulation of persistent infection. J. gen. Virol. 1977;35:265–279. doi: 10.1099/0022-1317-35-2-265. [DOI] [PubMed] [Google Scholar]
  • 14.Preble O. T., Youngner J. S. Selection of temperature sensitive mutants during persistent infection: Role in maintenance of persistence Newcastle disease virus infections of L cells. J. Virol. 1973;12:481–491. doi: 10.1128/jvi.12.3.481-491.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 15.Preble O. T., Youngner J. S. Temperature sensitive mutant viruses and the etiology of chronic and inapparent infections. J. inf. Dis. 1975;131:467–473. doi: 10.1093/infdis/131.4.467. [DOI] [PubMed] [Google Scholar]
  • 16.Rima B. K., Davidson W. B., Martin S. J. The role of defective interfering particles in persistent infection of vero cells by measles virus. J. gen. Virol. 1977;35:89–97. doi: 10.1099/0022-1317-35-1-89. [DOI] [PubMed] [Google Scholar]
  • 17.Robb J. A., Bond C. W. Coronaviridae. In: Fraenkel-Conrat H., Wagner R. R., editors. Comprehensive Virology, Vol. 14. New York: Plenum Press; 1979. pp. 193–247. [Google Scholar]
  • 18.Stohlman S. A., Sakaguchi A. Y., Weiner L. P. Characterization of the cold-sensitive murine hepatitis virus mutants rescued from latently infected cells by cell fusion. Virology. 1979;98:448–455. doi: 10.1016/0042-6822(79)90567-1. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 19.Stohlman S. A., Weiner L. P. Stability of neurotropic mouse hepatitis virus (JHM strain) during chronic infection of neuroblastoma cells. Arch. Virol. 1978;57:53–61. doi: 10.1007/BF01315637. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 20.Tanaka R., Iwasaki Y., Koprowski H. Intracisternal virus-like particles in brain of a multiple sclerosis patient. J. Neurol. Sci. 1976;28:121–126. doi: 10.1016/0022-510X(76)90053-8. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 21.Truant A. L., Hallum J. V. A persistent infection of baby hamster kidney-21 cells with mumps virus and the role of temperature sensitive variants. J. med. Virol. 1977;1:49–67. doi: 10.1002/jmv.1890010108. [DOI] [PubMed] [Google Scholar]
  • 22.Youngner J. S., Dubovi E. J., Quagliana D. D., Kelly M., Preble O. T. Role of temperature sensitive mutants in persistent infections initiated with vesicular stomatitis virus. J. Virol. 1976;19:90–101. doi: 10.1128/jvi.19.1.90-101.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 23.Zhdanov V. M. Integration of genomes of infectious RNA viruses. Intervirol. 1975;6:128–132. doi: 10.1159/000149465. [DOI] [PubMed] [Google Scholar]

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