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. 1996;141(9):1677–1688. doi: 10.1007/BF01718291

Genetic grouping for the isolates of avian infectious bronchitis virus in Taiwan

C -H Wang 1, C -T Tsai 1
PMCID: PMC7086916  PMID: 8893790

Summary

In order to differentiate recent isolates of avian infectious bronchitis virus (IBV) in Taiwan, polymerase chain reaction (PCR), restriction fragment length polymorphism (RFLP), and direct sequencing methods were used to type 25 IBV Taiwan isolates. Two conserved sequences that flank the hypervariable region I (HVR I) in the N-terminus of S1 protein gene were chosen as primers. Sequences of 228–231 base pairs (bp) were amplified by PCR from 25 Taiwan isolates and 4 reference strains (H120, Conn, JMK, Holte). PCR products were digested with 5 restriction endonucleases,BsoFI,DdeI,MboII,AluI,RsaI, and different IBV isolates were grouped according to their RFLP patterns. The RFLP patterns of the 4 reference strains in this study matched the published sequences in GenBank. Except 1 vaccine strain, the other 24 Taiwan isolates were different from these 4 and 18 other IBV strains whose sequences were published. The data from PCR-RFLP and sequencing of IBV genomes showed that the 24 Taiwan isolates can be divided into 2 distinct groups, I and II. Seven RFLP patterns are identified in group I and only 1 in group II.

Keywords: Polymerase Chain Reaction, Polymerase Chain Reaction Product, Restriction Fragment Length Polymorphism, Reference Strain, Bronchitis

References

  • 1.Andreasen JR, Jr, Jackwood MW, Hilt DA. Polymerase chain reaction amplification of the genome of infectious bronchitis virus. Avian Dis. 1991;35:216–220. [PubMed] [Google Scholar]
  • 2.Binns MM, Boursnell MEG, Cavanagh D, Pappin DJC, Brown TDK. Cloning and sequencing of the gene encoding the spike protein of the coronavirus IBV. J Gen Virol. 1985;66:719–726. doi: 10.1099/0022-1317-66-4-719. [DOI] [PubMed] [Google Scholar]
  • 3.Binns MM, Boursnell MEG, Tomley FM, Brown TDK. Comparison of the spike precursor sequences of coronavirus IBV strains M41 and 6/82 with that of IBV Beaudette. J Gen Virol. 1986;67:2825–2831. doi: 10.1099/0022-1317-67-12-2825. [DOI] [PubMed] [Google Scholar]
  • 4.Brown TDK, Boursnell MEG, Binns MM. A leader sequence is present on mRNA A of avian infectious bronchitis virus. J Gen Virol. 1984;65:1437–1442. doi: 10.1099/0022-1317-65-8-1437. [DOI] [PubMed] [Google Scholar]
  • 5.Boursnell MEG, Brown TDK, Foulds IJ, Green PF, Tomley FM, Binns MM. Completion of the sequence of the genome of the coronavirus avian infectious bronchitis virus. J Gen Virol. 1987;68:57–77. doi: 10.1099/0022-1317-68-1-57. [DOI] [PubMed] [Google Scholar]
  • 6.Cavanagh D, Davis PJ. Sequence analysis of strains of avian infectious bronchitis coronavirus isolated during the 1960s in the U.K. Arch Virol. 1992;130:471–476. doi: 10.1007/BF01309675. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 7.Cavanagh D, Darbyshire JH, Davis P, Peters RW. Induction of humoral neutralizing and haemagglutination inhibiting antibody by the spike protein of avian infectious bronchitis virus. Avian Pathol. 1984;13:573–583. doi: 10.1080/03079458408418556. [DOI] [PubMed] [Google Scholar]
  • 8.Dawson PS, Gough RE. Antigenic variation in strains of avian infectious bronchitis virus. Arch Ges Virusforsch. 1971;34:32–39. doi: 10.1007/BF01250243. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 9.Kant A, Koch G, van Roozelaar DJ, Kusters JG, Poelwijk FAJ, van der Zeijst BAM. Location of antigenic sites defined by neutralizing monoclonal antibodies on the S1 avian infectious virus glycopolypeptides. J Gen Virol. 1992;73:591–596. doi: 10.1099/0022-1317-73-3-591. [DOI] [PubMed] [Google Scholar]
  • 10.King DJ, Cavanagh D. Infectious bronchitis. In: Calnek BW, editor. Disease of poultry. 9th ed. Ames: Iowa State University Press; 1991. pp. 471–484. [Google Scholar]
  • 11.Koch G, Hartog L, Kant A, van Roozelaar DJ. Antigenic domains on the peplomer protein of avian infectious bronchitis virus: correlation with biological functions. J Gen Virol. 1990;71:1929–1935. doi: 10.1099/0022-1317-71-9-1929. [DOI] [PubMed] [Google Scholar]
  • 12.Kwon HM, Jackwood MW, Gelb J., Jr Differentiation of infectious bronchitis virus serotypes using polymerase chain reaction and restriction fragment length polymorphism analysis. Avian Dis. 1993;37:194–202. [PubMed] [Google Scholar]
  • 13.Lin Z, Kato A, Kudou Y, Ueda S. A new typing method for the avian infectious bronchitis virus using polymerase chain reaction and restriction enzyme fragment length polymorphism. Arch Virol. 1991;116:19–31. doi: 10.1007/BF01319228. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 14.Wang L, Junker D, Collisson EW. Evidence of natural recombination within the S1 gene of infectious bronchitis virus. Virology. 1993;192:710–716. doi: 10.1006/viro.1993.1093. [DOI] [PubMed] [Google Scholar]
  • 15.Wang L, Junker D, Hock L, Ebiary E, Collisson EW. Evolutionary implications of genetic variations in the S1 gene of infectious bronchitis virus. Virus Res. 1994;34:327–338. doi: 10.1016/0168-1702(94)90132-5. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 16.Williams AK, Wang L, Sneed LW, Collisson EW. Comparative analyses of the nucleocapsid genes of several strains of infectious bronchitis virus and other coronaviruses. Virus Res. 1992;25:231–222. doi: 10.1016/0168-1702(92)90135-V. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 17.Winterfield RW, Thacker HL, Badylak SF. Effects of sub-type variations in Massachusetts-type infectious bronchitis virus when applied in vaccines. Poultry Sci. 1984;63:248–250. doi: 10.3382/ps.0630246. [DOI] [PubMed] [Google Scholar]
  • 18.Zwaagstra KA, van der Zeijst BAM, Kusters JG. Rapid detection and identification of avian infectious bronchitis virus. J Clin Microbiol. 1992;30:79–84. doi: 10.1128/jcm.30.1.79-84.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]

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