Skip to main content
NCBI home page
Springer Nature - PMC COVID-19 Collection logoLink to Springer Nature - PMC COVID-19 Collection
. 1992;127(1):257–269. doi: 10.1007/BF01309589

Partial DNA cloning and sequencing of a canine parvovirus vaccine strain: application of nucleic acid hybridization to the diagnosis of canine parvovirus disease

M Remond 1, P Boireau 1, F Lebreton 1
PMCID: PMC7086918  PMID: 1456893

Summary

The cloning and sequencing of anEco RI-Pst I fragment derived from the replicative form of a canine parvovirus (CPV) vaccine strain are reported. The variability of the 5′ end of NS 1 protein gene in the genome is confirmed by comparison with previously determined DNA sequences. A 15 nucleotide deletion was also observed in this vaccine strain. In order to improve CPV diagnosis, radioactively labelled RNA or DNA and biotin labelled DNA obtained by random priming of the recombinant plasmid were used as probes mainly on gut or stool samples from naturally infected dogs. Results of filter hybridization correlated well with histopathological diagnosis of parvovirus infection and with hemagglutination tests performed on dog faeces. We propose that nucleic acid hybridization may be an alternative diagnostic method to ascertain the presence of CPV, especially in frozen samples.

Keywords: Biotin, Stool Sample, Vaccine Strain, Histopathological Diagnosis, Nucleotide Deletion

References

  • 1.Anton IA, Lane DP. Non-structural protein 1 of parvoviruses: homology to purine nucleotide using proteins and early proteins of papovaviruses. Nucleic Acid Res. 1986;14:7813. doi: 10.1093/nar/14.19.7813. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 2.Astell CR, Gardiner EM, Tattersall P. DNA sequence of the lymphotropic variant of minute virus of mice, mvm(i) and comparison with the DNA sequence of the fibrotropic prototype strain. J Virol. 1986;57:656–669. doi: 10.1128/jvi.57.2.656-669.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 3.Astell CR, Thomson M, Merchlinsky M, Ward DC. The complete DNA sequence of minute virus of mice, an autonomous parvovirus. Nucleic Acid Res. 1983;11:999–1018. doi: 10.1093/nar/11.4.999. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 4.Azzi A, Zakrzewska K, Gentilomi G, Musiani M, Zerbini M. Detection of B 19 parvovirus infections by a dot-blot hybridization assay using a digoxigenin-labelled probe. J Virol Methods. 1990;27:125–134. doi: 10.1016/0166-0934(90)90129-4. [DOI] [PubMed] [Google Scholar]
  • 5.Boireau P, Crucière C, Laporte J. Nucleotide sequence of the glycoprotein S gene of bovine enteric coronavirus and comparison with the S proteins of two mouse hepatitis virus strains. J Gen Virol. 1990;71:487–492. doi: 10.1099/0022-1317-71-2-487. [DOI] [PubMed] [Google Scholar]
  • 6.Carlson J, Rushlow K, Maxwell I, Maxwell F, Winston S, Hahn W. Cloning and sequence of DNA encoding structural proteins of autonomous parvovirus feline panleukopenia virus. J Virol. 1985;55:574–582. doi: 10.1128/jvi.55.3.574-582.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 7.Carmichael LE, Joubert JC, Pollock RVH. Hemagglutination by canine parvovirus: serologic studies and diagnostic applications. Am J Vet Res. 1980;41:784–791. [PubMed] [Google Scholar]
  • 8.Carmichael LE, Joubert JC, Pollock RVH. A modified live canine parvovirus strain with novel plaque characteristics. I. Viral attenuation and dog response. Cornell Vet. 1981;71:408–427. [PubMed] [Google Scholar]
  • 9.Chen KC, Shull BC, Moses EA, Lederman M, Stout ER, Bates RC. Complete nucleotide sequence and genome organization of bovine parvovirus. J Virol. 1986;60:1085–1097. doi: 10.1128/jvi.60.3.1085-1097.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 10.Eiden JJ, Firoozmand F, Sato S, Vonderfecht SL, Zhao-Yin F, Yolken RH. Detection of group B rotavirus in fecal specimens by dot hybridization with a cloned cDNA probe. J Clin Microbiol. 1989;27:422–426. doi: 10.1128/jcm.27.3.422-426.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 11.Feinberg AP, Vogelstein B. A technique for radiolabelling DNA restriction endonuclease fragments to high specific activity. Anal Biochem. 1983;132:6–13. doi: 10.1016/0003-2697(83)90418-9. [DOI] [PubMed] [Google Scholar]
  • 12.Hammond G, Hannan C, Yeh T, Fisher K, Mauthe G, Straus SE. DNA hybridization for diagnosis of enteric adenovirus infection from directly spotted human fecal specimens. J Clin Microbiol. 1987;25:1881–1885. doi: 10.1128/jcm.25.10.1881-1885.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 13.Hanahan D. Techniques for transformation ofE. coli. In: Glover DM, editor. DNA cloning, vol I: a practical approach. Oxford: IRL Press; 1985. pp. 109–135. [Google Scholar]
  • 14.Hirt B. Selective extraction of polyoma DNA from infected mouse cell cultures. J Mol Biol. 1967;26:365–369. doi: 10.1016/0022-2836(67)90307-5. [DOI] [PubMed] [Google Scholar]
  • 15.Kopecka H, Prevot J, Girard M, Fuchs F, Aymard M. Intérêt des sondes ARNc (Ribosondes) synthétisées in vitro dans la détection des enterovirus par hybridation moléculaire. Ann Inst Past Virol. 1988;139:218–225. doi: 10.1016/s0769-2617(88)80019-4. [DOI] [PubMed] [Google Scholar]
  • 16.Krell PJ, Salas T, Johnson RP. Mapping of porcine parvovirus DNA and development of a diagnostic DNA probe. Vet Microbiol. 1988;17:29–43. doi: 10.1016/0378-1135(88)90077-6. [DOI] [PubMed] [Google Scholar]
  • 17.Lim H, Pene JJ. Optimal conditions for supercoil DNA sequencing withEscherichia coli DNA polymerase I large fragment. Gene Anal Tech. 1988;5:32–39. doi: 10.1016/0735-0651(88)90024-6. [DOI] [PubMed] [Google Scholar]
  • 18.Linne T. Diagnosis of pseudorabies virus infection in pigs with specific DNA probes. Res Vet Sci. 1987;43:150–156. [PubMed] [Google Scholar]
  • 19.Maniatis T, Fritsch EF, Sambrook J. Molecular cloning. A laboratory manual. Cold Spring Harbor: Cold Spring Harbor Laboratory; 1982. [Google Scholar]
  • 20.Martyn SC, Davidson BE, Studdert M. Nucleotide sequence of feline panleukopenia virus: comparison with canine parvovirus identifies host-specific differences. J Gen Virol. 1990;71:2747–2753. doi: 10.1099/0022-1317-71-11-2747. [DOI] [PubMed] [Google Scholar]
  • 21.McMaster GK, Beard P, Engers MD, Hirt B. Characterization of an immunosuppressive parvovirus related to the minute virus of mice. J Virol. 1981;38:317–326. doi: 10.1128/jvi.38.1.317-326.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 22.McMaster GK, Tratschin JD, Siegl G. Comparison of canine parvovirus with mink enteritis virus by restriction site mapping. J Virol. 1981;38:368–371. doi: 10.1128/jvi.38.1.368-371.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 23.Mengeling WL, Paul PS, Bunn TO, Rippath JF. Antigenic relationships among autonomous parvoviruses. J Gen Virol. 1986;67:2839–2844. doi: 10.1099/0022-1317-67-12-2839. [DOI] [PubMed] [Google Scholar]
  • 24.Nelson DT, Eustis SL, McAdaragh JP, Stotz I. Lesions of spontaneous canine viral enteritis. Vet Pathol. 1979;16:680–686. doi: 10.1177/030098587901600606. [DOI] [PubMed] [Google Scholar]
  • 25.Orth G, Favre M, Croissant O. Characterization of a new type of human papillomavirus that causes skin warts. J Virol. 1977;24:108–120. doi: 10.1128/jvi.24.1.108-120.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 26.Paradiso PR. Infectious process of the parvovirus H 1: correlation of protein content, particle density and viral infectivity. J Virol. 1981;39:800–807. doi: 10.1128/jvi.39.3.800-807.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 27.Parrish CR (1991) EMBL data bank access number, M 38245
  • 28.Pletcher JM, Toft JD, Frey RM, Harold WC. Histopathologic evidence for parvovirus infection in dogs. J Am Vet Med Assoc. 1979;175:825–828. [PubMed] [Google Scholar]
  • 29.Ranz AI, Manclus JJ, Diaz-Aroca E, Casal JI. Porcine parvovirus: DNA sequence and genome organization. J Gen Virol. 1989;70:2541–2553. doi: 10.1099/0022-1317-70-10-2541. [DOI] [PubMed] [Google Scholar]
  • 30.Reed AP, Jones EV, Miller TJ. Nucleotide sequence and genome organization of canine parvovirus. J Virol. 1988;62:266–276. doi: 10.1128/jvi.62.1.266-276.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 31.Rhode SL, Paradiso PR. Parvovirus genome: nucleotide sequence of H 1 and mapping of its genes by hybrid-arrested translation. J Virol. 1983;45:173–184. doi: 10.1128/jvi.45.1.173-184.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 32.Rhode SL. III. Nucleotide sequence of the coat protein gene of canine parvovirus. J Virol. 1985;54:630–633. doi: 10.1128/jvi.54.2.630-633.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 33.Sahli R, McMaster GK, Hirt B. DNA sequence comparison between two tissue-specific variants of the autonomous parvovirus, minute virus of mice. Nucleic Acid Res. 1985;13:3617–3633. doi: 10.1093/nar/13.10.3617. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 34.Salimans MM, Holsappel S, Van De Rijke FM, Jiwa NM, Raap AK, Weiland HT. Rapid detection of human parvovirus B 19 DNA by dot-hybridization and the polymerase chain reaction. J Virol Methods. 1989;23:19–28. doi: 10.1016/0166-0934(89)90085-2. [DOI] [PubMed] [Google Scholar]
  • 35.Sambrook J, Fritsch EF, Maniatis T. Molecular cloning. A laboratory manual. 2nd edn. Cold Spring Harbor: Cold Spring Harbor Laboratory; 1989. [Google Scholar]
  • 36.Shade RO, Blundell MC, Cotmore SF, Tattersall P, Astell CR. Nucleotide sequence and genome organization of human parvovirus B 19 isolated from the serum of a child during aplastic crisis. J Virol. 1986;58:921–936. doi: 10.1128/jvi.58.3.921-936.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 37.Siegl GS, Bates RC, Berns KI, Carter BJ, Kelly OC, Kurstak E, Tattersall P. Characteristics and taxonomy of Parvoviridae. Intervirology. 1985;23:61–73. doi: 10.1159/000149587. [DOI] [PubMed] [Google Scholar]

Articles from Archives of Virology are provided here courtesy of Nature Publishing Group

RESOURCES