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. 1991;116(1):19–31. doi: 10.1007/BF01319228

A new typing method for the avian infectious bronchitis virus using polymerase chain reaction and restriction enzyme fragment length polymorphism

Z Lin 1, A Kato 1, Y Kudou 1, S Ueda 1
PMCID: PMC7086981  PMID: 1672064

Summary

Two primers with the length of 22 bases each and 400 bases apart on the spike protein gene of avian infectious bronchitis virus (IBV) were prepared. Using these primers, the genome RNA from twelve strains of the various serotypes were reverse-transcribed to cDNA and amplified by polymerase chain reaction (PCR). With all strains, 400 base DNA was amplified, indicating that there were no apparent insertions or deletions in this region. However, the amplified DNA showed different cleavage patterns by the restriction enzymes. These 12 strains were classified into 5 groups. The strain typing based on a comparison of the cleavage patterns was consistent with the previous serological typing. This study thus provides a simple and rapid method for typing of IBV.

Keywords: Restriction Enzyme, Fragment Length, Strain Typing, Length Polymorphism, Fragment Length Polymorphism

References

  • 1.Alexander DJ, Chettle NJ. Procedures for the haemagglutination inhibition tests for avian infectious bronchitis virus. Avian Pathol. 1977;6:9–17. doi: 10.1080/03079457708418208. [DOI] [PubMed] [Google Scholar]
  • 2.Binns MM, Boursnell MEG, Cavanagh D, Pappin DJC, Brown TDK. Cloning and sequencing of the gene encoding the spike protein of the coronavirus IBV. J Gen Virol. 1985;66:719–726. doi: 10.1099/0022-1317-66-4-719. [DOI] [PubMed] [Google Scholar]
  • 3.Binns MM, Boursnell MEG, Tomley FM, Brown TDK. Comparison of the spike precursor sequences of coronavirus IBV strain M41 and 6/82 with that of IBV Beaudette. J Gen Virol. 1986;67:2835–2831. doi: 10.1099/0022-1317-67-12-2825. [DOI] [PubMed] [Google Scholar]
  • 4.Brown TDK, Boursnell MEG. Avian infectious bronchitis virus genomic RNA contains sequences homologies at the intergenic boundaries. Virus Res. 1984;1:15–24. [Google Scholar]
  • 5.Brown TDK, Boursnell MEG, Binns MM. A leader sequence is present on mRNA A of avian infectious bronchitis virus. J Gen Virol. 1984;65:1437–1442. doi: 10.1099/0022-1317-65-8-1437. [DOI] [PubMed] [Google Scholar]
  • 6.Carman WF, Kidd AH. An assessment of optimal conditions for amplification of HIV cDNA usingThermus aquaticus polymerase. J Virol Methods. 1989;23:277–290. doi: 10.1016/0166-0934(89)90160-2. [DOI] [PubMed] [Google Scholar]
  • 7.Cavanagh D. Structural polypeptides of coronavirus IBV. J Gen Virol. 1981;53:93–103. doi: 10.1099/0022-1317-53-1-93. [DOI] [PubMed] [Google Scholar]
  • 8.Cavanagh D. Coronavirus IBV: further evidence that the surface projections are associated with two glycopolypeptides. J Gen Virol. 1983;64:1787–1791. doi: 10.1099/0022-1317-64-8-1787. [DOI] [PubMed] [Google Scholar]
  • 9.Cavanagh D. Coronavirus IBV: structural characterization of the spike protein. J Gen Virol. 1983;64:2577–2583. doi: 10.1099/0022-1317-64-12-2577. [DOI] [PubMed] [Google Scholar]
  • 10.Cavanagh D, Davis PJ, Darbyshire JH, Peters RW. Coronavirus IBV: virus retaining spike glycopolypeptide S2 but not S1 is unable to induce virus-neutralizing or haemagglutination-inhibiting antibody, or induce chicken tracheal protection. J Gen Virol. 1986;67:1435–1442. doi: 10.1099/0022-1317-67-7-1435. [DOI] [PubMed] [Google Scholar]
  • 11.Cavanagh D, Davis PJ. Evolution of avian coronavirus IBV: sequence of the matrix glycoprotein gene and intergenic region of several serotypes. J Gen Virol. 1988;69:621–629. doi: 10.1099/0022-1317-69-3-621. [DOI] [PubMed] [Google Scholar]
  • 12.Cavanagh D, Davis DJ, Mockett APA. Amino acids within hypervariable region 1 of avian coronavirus IBV (Massachusetts serotype) spike glycoprotein are associated with neutralization epitopes. Virus Res. 1988;11:141–150. doi: 10.1016/0168-1702(88)90039-1. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 13.Clewley JP, Morser J, Avery RJ, Lomniczi B. Oligonucleotide fingerprinting of ribonucleic acids of infectious bronchitis virus strain. Infect Immun. 1981;32:1227–1233. doi: 10.1128/iai.32.3.1227-1233.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 14.Cook JKA. The classification of new serotypes of infectious bronchitis virus isolated from poultry flocks in Britain 1981 and 1983. Avian Pathol. 1984;13:733–741. doi: 10.1080/03079458408418570. [DOI] [PubMed] [Google Scholar]
  • 15.Cook JKA, Darbyshire JH, Peters RW. The use of chicken tracheal organ cultures for the isolation and assay of avian infectious bronchitis virus. Arch Virol. 1976;50:109–118. doi: 10.1007/BF01318005. [DOI] [PubMed] [Google Scholar]
  • 16.Cowen BS, Hitchner SB. Serotyping of avian infectious bronchitis viruses by the virus neutralization test. Avian Dis. 1975;19:583–595. [PubMed] [Google Scholar]
  • 17.Cunningham CH. Immunologic methods in avian research: neutralization test. Avian Dis. 1973;17:227–235. [PubMed] [Google Scholar]
  • 18.Darbyshire JH, Rowell JG, Cook JKA, Peters RW. Taxonomic studies on strains of avian infectious bronchitis virus using neutralization tests in tracheal organ cultures. Arch Virol. 1979;61:227–238. doi: 10.1007/BF01318057. [DOI] [PubMed] [Google Scholar]
  • 19.Davelaar FG, Kouwenhoven B, Burger AG. Occurrence and significance of infectious bronchitis virus variant strains in egg and broiler production in the Netherlands. Vet Q. 1984;6:114–120. doi: 10.1080/01652176.1984.9693924. [DOI] [PubMed] [Google Scholar]
  • 20.Hofstad MS. Antigenic differences among isolates of avian infectious bronchitis virus. Am J Vet Res. 1958;19:740–743. [PubMed] [Google Scholar]
  • 21.Hopkins SR. Serological comparisons of strains of infectious bronchitis virus using plaque-purified isolants. Avian Dis. 1974;18:231–239. [PubMed] [Google Scholar]
  • 22.Johnson RB, Marquardt WW. The neutralizing characteristics of strains of infectious bronchitis virus as measured by constant-virus variable-serum method in chicken tracheal culture. Avian Dis. 1975;19:82–90. [PubMed] [Google Scholar]
  • 23.King DJ. Identification of recent infectious bronchitis virus isolated that are serologically different from current vaccine strains. Avian Dis. 1988;32:362–364. [PubMed] [Google Scholar]
  • 24.King DJ, Hopkins SR. Evaluation of the hemagglutination-inhibition test for measuring the response of chickens to avian infectious bronchitis virus vaccination. Avian Dis. 1983;27:100–103. [PubMed] [Google Scholar]
  • 25.King DJ, Hopkins SR. Rapid serotyping of infectious bronchitis virus isolates with the hemagglutination-inhibition test. Avian Dis. 1984;28:727–733. [PubMed] [Google Scholar]
  • 26.Kusters JG, Niesters HGM, Bleumink-Pluym NMC, Davelaar FG, Horzinek MC, Van Der Zeijst BAM. Molecular epidemiology of infectious bronchitis virus in the Netherlands. J Gen Virol. 1987;68:343–352. doi: 10.1099/0022-1317-68-2-343. [DOI] [PubMed] [Google Scholar]
  • 27.Kusters JG, Niesters HGM, Lenstra JA, Horzinek MC, Van Der Zeijst BAM. Phylogeny of antigenic variants of avian coronavirus IBV. Virology. 1989;169:217–221. doi: 10.1016/0042-6822(89)90058-5. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 28.Lenstra JA, Kusters JG, Koch G, Van Der Zeijst BAM. Antigenicity of the peplomer protein of infectious bronchitis virus. Mol Immunol. 1989;26:7–15. doi: 10.1016/0161-5890(89)90014-X. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 29.Mockett APA, Cavanagh D, Brown TDK. Monoclonal antibodies to the S1 spike and membrane proteins of avian infectious bronchitis coronavirus strain Massachusetts M41. J Gen Virol. 1984;65:2281–2286. doi: 10.1099/0022-1317-65-12-2281. [DOI] [PubMed] [Google Scholar]
  • 30.Nagy E, Lomniczi B. Polypeptide pattern of infectious bronchitis virus serotypes fall into two categories. Arch Virol. 1979;61:341–345. doi: 10.1007/BF01315022. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 31.Niesters HGM, Lenstra JA, Spaan WJM, Zijderveld AJ, Bleumink-Pluym NMC, Hong F, Van Scharrenberg GJM, Horzinek MC, Van Der Zeijst BAM. The peplomer protein sequences of the M41 strain of coronavirus IBV and its comparison with Beaudette strains. Virus Res. 1986;5:253–263. doi: 10.1016/0168-1702(86)90022-5. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 32.Purchase HG, Cunningham CH, Burmester BR. Identification and epizootiology of infectious bronchitis in a closed flock. Avian Dis. 1966;10:111–121. [PubMed] [Google Scholar]
  • 33.Saiki RK, Scharf S, Faloona F, Mullis KB, Horn GT, Erlich HA, Arnheim N. Enzymatic amplification of beta-globin genomic sequences and restriction site analysis for diagnosis of sickle cell anemia. Science. 1985;230:1350–1354. doi: 10.1126/science.2999980. [DOI] [PubMed] [Google Scholar]
  • 34.Saiki RK, Gelfand DH, Stoffel S, Scharf SJ, Higuchi R, Horn GT, Mullis KB, Erlich HA. Primer-directed enzymatic amplification of DNA with a thermostable DNA polymerase. Science. 1988;239:487–491. doi: 10.1126/science.2448875. [DOI] [PubMed] [Google Scholar]
  • 35.Schochetman G, Stevens RH, Simpson RW. Presence of infectious polyadenylated RNA in the coronavirus avian bronchitis virus. Virology. 1977;77:772–782. doi: 10.1016/0042-6822(77)90498-6. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 36.Snijders PFJ, Van Den Brule AJC, Schrijnemakers HFJ, Snow G, Meijer CJLM, Walboomers JMM. The use of general primers in the polymerase chain reaction permits the detection of a broad spectrum of human papillomavirus genotypes. J Gen Virol. 1990;71:173–181. doi: 10.1099/0022-1317-71-1-173. [DOI] [PubMed] [Google Scholar]
  • 37.Siddell S, Wege H, Ter Meulen V. The biology of coronaviruses. J Gen Virol. 1983;64:761–776. doi: 10.1099/0022-1317-64-4-761. [DOI] [PubMed] [Google Scholar]
  • 38.Stern DF, Sefton BM. Coronavirus multiplication: location of genes for virion proteins on the avian infectious bronchitis virus genome. J Virol. 1984;50:22–29. doi: 10.1128/jvi.50.1.22-29.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 39.Sutou S, Sato S, Okabe T, Nakai M, Sasaki N. Cloning and sequencing of genes encoding structural proteins of infectious bronchitis virus. Virology. 1988;165:589–595. doi: 10.1016/0042-6822(88)90603-4. [DOI] [PubMed] [Google Scholar]
  • 40.Winterfield RW, Hitchner SB. Etiology of an infectious nephritis-nephrosis syndrome of chickens. Am J Vet Res. 1962;23:1273–1279. [PubMed] [Google Scholar]

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