Summary
Bovine coronavirus readily multiplied and induced marked cytopathic effect in BEK-1 cultures, thus providing a sensitive, practical assay system for viral infectivity and neutralizing antibody and a satisfactory source of the virus.
Keywords: Infectious Disease, Viral Infectivity, Assay System, Neutralize Antibody, Cytopathic Effect
Footnotes
With 1 Figure
References
- 1.Inaba Y., Kurogi H., Takahashi E., Sato K., Tanaka Y., Goto Y., Omori T., Matumoto M. Isolation and properties of bovine parvovirus type 1 from Japanese calves. Arch. ges. Virusforsch. 1973;42:54–66. doi: 10.1007/BF01250507. [DOI] [PubMed] [Google Scholar]
- 2.Matumoto M., Inaba Y., Tanaka Y., Ito H., Omori T. Behavior of bovine ephemeral fever virus in laboratory animals and cell cultures. Japan. J. Microbiol. 1970;14:413–421. doi: 10.1111/j.1348-0421.1970.tb00541.x. [DOI] [PubMed] [Google Scholar]
- 3.Mebus C. A., Stair E. L., Rhodes M. B., Twiehaus M. J. Neonatal calf diarrhea: propagation, attenuation and characteristics of a coronavirus-like agent. Amer. J. vet. Res. 1973;34:145–150. [PubMed] [Google Scholar]
- 4.Mebus C. A., Stair E. L., Rhodes M. B., Twiehaus M. J. Pathology of neonatal calf diarrhea induced by a coronavirus-like agent. Vet. Path. 1973;10:45–64. doi: 10.1177/030098587301000105. [DOI] [PubMed] [Google Scholar]
- 5.Mebus C. A., White R. G., Stair E. L., Rhodes M. B., Twiehaus M. J. Neonatal calf diarrhea: results of a field trial using a reo-like virus vaccine. Vet. Med./Small Anim. Clin. 1972;67:173–174. [PubMed] [Google Scholar]
- 6.Stair E. L., Rhodes M. B., White R. G., Mebus C. A. Neonatal calf diarrhea: purification and electron microscopy of a coronavirus-like agent. Amer. J. vet. Res. 1972;33:1147–1156. [PubMed] [Google Scholar]