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. 1978;57(1):43–51. doi: 10.1007/BF01315636

Replication of an enteric bovine coronavirus in intestinal organ cultures

Janice C Bridger 1, E O Caul 2, S I Egglestone 3
PMCID: PMC7087085  PMID: 655865

Summary

A coronavirus isolated in tracheal organ cultures from the faeces of a calf with diarrhoea readily multiplied on passage in intestinal organ cultures. Evidence for multiplication was obtained by the production of viral haemagglutinin in organ culture fluids and the presence of immunofluorescence and viral particles in the columnar epithelial cells of the villi. Thus virus multiplication was studiedin vitro in the cell type in which it multiplies naturally.

Keywords: Epithelial Cell, Infectious Disease, Organ Culture, Virus Multiplication, Viral Particle

Footnotes

With 9 Figures

References

  • 1.Caul E. O., Clarke S. K. R. Coronavirus propagated from patient with non-bacterial gastroenteritis. Lancet. 1975;ii:853–854. doi: 10.1016/S0140-6736(75)90363-3. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 2.Caul E. O., Egglestone S. I. Further studies on human enteric coronaviruses. Arch. Virol. 1977;54:107–117. doi: 10.1007/BF01314383. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 3.Dolin R., Blacklow N. R., Malmgren R. A., Chanock R. M. Establishment of human foetal intestinal organ cultures for growth of viruses. J. inf. Dis. 1970;122:227–231. doi: 10.1093/infdis/122.3.227. [DOI] [PubMed] [Google Scholar]
  • 4.Egglestone, S. I.: Laboratory investigation into the aetiology of non-bacterial gastroenteritis. Ph. D. Thesis, University of Bristol, 1968.
  • 5.McIntosh K. Coronaviruses: A comparative review. Curr. Top. Microbiol. Immunol. 1974;63:85–129. [Google Scholar]
  • 6.Mebus C. A., Stair E. L., Rhodes M. B., Twiehaus M. J. Neonatal calf diarrhea: propagation, attenuation and characteristics of a coronavirus-like agent. Amer. J. vet. Res. 1973;34:145–150. [PubMed] [Google Scholar]
  • 7.Rubenstein D., Tyrrell D. A. J. Growth of viruses in organ cultures of intestine. Brit. J. exp. Pathol. 1970;51:210–216. [PMC free article] [PubMed] [Google Scholar]
  • 8.Rubenstein D., Tyrrell D. A. J., Derbyshire J. B., Collins A. P. Growth of porcine transmissible gastroenteritis (TGE) virus in organ cultures of pig tissue. Nature. 1970;227:1348–1349. doi: 10.1038/2271348a0. [DOI] [PubMed] [Google Scholar]
  • 9.Thornhill T. S., Kalica A. R., Wyatt R. G., Kapikian A. Z., Chanock R. M. Pattern of shedding of the Norwalk particle in stools during experimentally induced gastroenteritis in volunteers as determined by immune electron microscopy. J. inf. Dis. 1975;132:28–34. doi: 10.1093/infdis/132.1.28. [DOI] [PubMed] [Google Scholar]
  • 10.Woode G. N., Bridger J. C. Viral enteritis of calves. Vet. Rec. 1975;96:85–88. doi: 10.1136/vr.96.4.85. [DOI] [PubMed] [Google Scholar]
  • 11.Wyatt R. G., Kapikian A. Z., Thornhill T. S., Sereno M. M., Kim H. W., Chanock R. M. In vitro cultivation in human foetal intestinal organ culture of a reovirus-like agent associated with non-bacterial gastroenteritis in infants and children. J. inf. Dis. 1974;130:523–528. doi: 10.1093/infdis/130.5.523. [DOI] [PubMed] [Google Scholar]
  • 12.Wyatt R. G., Gill V. W., Sereno M. M., Kalica A. R., Van Kirk D. H., Chanock R. M., Kapikian A. Z. Probablein vitro cultivation of human reovirus-like agent of infantile diarrhoea. Lancet. 1976;i:98–99. doi: 10.1016/s0140-6736(76)90202-6. [DOI] [PubMed] [Google Scholar]

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