Summary
Organ cultures of mouse trachea were infected with some indigenous mouse viruses. Mengovirus and reovirus type 3 grew to high titer; inocula of, respectively, 102 and 103 TCID50 were required to initiate infection. Organ cultures supported also the growth of mouse hepatitis viruses, MHV-3 and MHV-S, though to a lesser extent. Viral production was noted for periods of as long as two weeks. None of the viruses had a noticeable effect on the ciliary activity or acquired such capacity on serial passage in organ cultures.
Keywords: Hepatitis, Infectious Disease, Organ Culture, Hepatitis Virus, High Titer
References
- 1.Akers T. G., Madin S. H., Schaffer F. L. The pathogenicity in mice of aerosols of encephalomyocarditis group viruses or their infectious nucleic acids. J. Immunol. 1968;100:120–127. [PubMed] [Google Scholar]
- 2.Calisher, C. H., and W. P.Rowe: Mouse hepatitis, reo-3, and the Theiler viruses. In: Viruses of Laboratory Rodents (R. Holdenried, ed.), Nat. Cancer Inst. Monograph No.20, 67–75 (1966). [PubMed]
- 3.Colter J. S., Campbell J. B., Hatch L. R. The pathogenicity to mice of three variants of mengo encephalomyelitis virus. J. cell. comp. Physiol. 1965;65:229 to 236. doi: 10.1002/jcp.1030650209. [DOI] [PubMed] [Google Scholar]
- 4.Eustatia J. M., van der Veen J. Viral replication in cultures of phytohemagglutinin-treated mouse lymphocytes. Proc. Soc. exp. Biol. (N.Y.) 1971;137:424 to 428. [Google Scholar]
- 5.Eustatia J. M., Maase E., van Helden P., van der Veen J. Viral replication in mouse macrophages. Arch. ges. Virusforsch. 1972;39:376–380. doi: 10.1007/BF01241017. [DOI] [PubMed] [Google Scholar]
- 6.Friedman R., Maenza R. M. Peroral infection and resistance to reinfection with encephalomyocarditis virus in the mouse. J. infect. Dis. 1968;118:125–134. doi: 10.1093/infdis/118.2.125. [DOI] [PubMed] [Google Scholar]
- 7.Gomatos P. J., Tamm I., Dales S., Franklin R. M. Reovirus type 3: physical characteristics and interaction with L cells. Virology. 1962;17:441–454. doi: 10.1016/0042-6822(62)90139-3. [DOI] [PubMed] [Google Scholar]
- 8.Hobbs T. R., Mascoli C. C. Studies on experimental infection of weanling mice with reoviruses. Proc. Soc. exp. Biol. (N.Y.) 1965;118:847–853. doi: 10.3181/00379727-118-29989. [DOI] [PubMed] [Google Scholar]
- 9.Hoorn B., Tyrrell D. A. J. On the growth of certain “newer” respiratory viruses in organ cultures. Brit. J. exp. Path. 1965;46:109–118. [PMC free article] [PubMed] [Google Scholar]
- 10.Hoorn B., Tyrrell D. A. J. Organ cultures in virology. Progr. med. Virol. 1969;11:408–450. [PubMed] [Google Scholar]
- 11.Kärber G. Beitrag zur kollektiven Behandlung pharmakologischer Reihenversuche. Naunyn-Schmiedeberg's Arch. exp. Path. Pharmak. 1931;162:480–487. [Google Scholar]
- 12.Mcintosh K., Dees J. H., Becker W. B., Kapikian A. Z., Chanock R. M. Recovery in tracheal organ cultures of novel viruses from patients with respiratory disease. Proc. nat. Acad. Sci. (Wash.) 1967;57:933–940. doi: 10.1073/pnas.57.4.933. [DOI] [PMC free article] [PubMed] [Google Scholar]
- 13.Parker, J. C., R. W.Tennant, and T. G.Ward: Prevalence of viruses in mouse colonies. In: Viruses of Laboratory Rodents (R. Holdenried, ed.), Nat. Cancer Inst. Monograph No.20, 25–36 (1966). [PubMed]
- 14.Prato C. M., Akers T. G. The immunologic response of the mouse to aerosols of an attenuated encephalomyocarditis virus strain. J. Immunol. 1969;103:79–86. [PubMed] [Google Scholar]
- 15.Tyrrell D. A. J., Bynoe M. L. Cultivation of a novel type of common-cold virus in organ cultures. Brit. med. J. 1965;i:1467–1470. doi: 10.1136/bmj.1.5448.1467. [DOI] [PMC free article] [PubMed] [Google Scholar]
- 16.Tyrrell D. A. J., Bynoe M. L., Hoorn B. Cultivation of “difficult” viruses from patients with common colds. Brit. med. J. 1968;i:606–610. doi: 10.1136/bmj.1.5592.606. [DOI] [PMC free article] [PubMed] [Google Scholar]
- 17.Willems F. T. C., van der Veen J. Growth of Sendai virus in organ cultures of mouse tissue. Arch. ges. Virusforsch. 1968;23:148–156. doi: 10.1007/BF01242123. [DOI] [PubMed] [Google Scholar]