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. 1981;67(4):283–295. doi: 10.1007/BF01314832

Antigen requirements and specificity of a microplate enzyme-linked immunosorbent assay (ELISA) for detecting infectious bronchitis viral antibodies in chicken serum

A Soula 1, Y Moreau 1
PMCID: PMC7087152  PMID: 6165342

Summary

The conditions of a rapid, indirect-enzyme linked immunosorbent assay for Infectious Bronchitis Virus (IBV) antibodies have been established. Optimal sensitivity was obtained using 10 µg/ml protein concentration of the Mass 41 strain purified from infected allantoic fluid. Specificity was demonstrated with Newcastle Disease Virus (NDV) antigen-antibody system. Negligible crossreactions were observed. After bromelain or lipase treatment IBV had an ELISA reactivity similar to untreated particles suggesting that peripheral constituents of IBV play a minor role when whole virus is adsorbed on solid phase.

The method offers a simple and specific antibody assay which could be used for the laboratory diagnosis of avian infectious bronchitis.

Keywords: Lipase, Newcastle Disease Virus, Newcastle Disease, Infectious Bronchitis Virus, Viral Antibody

Footnotes

With 8 Figures

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