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. 1975;161(1):3–10. doi: 10.1007/BF02120764

In vitro cultivation of cells from the chorioallantoic membrane of chick embryos

Dagmar Cursiefen 1, Hermann Becht 1
PMCID: PMC7087208  PMID: 165393

Abstract

By treatment of chorioallantoic membranes from embryonated eggs with collagenase and hyaluronidase before the conventional application of trypsin cells could be grown in culture which supported growth of a large variety of myxoviruses, herpesviruses, avian reoviruses and the infectious bronchitis virus of chickens. The cultures could be used for sensitive plaque assays and neutralization tests.

Keywords: Trypsin, Collagenase, Bronchitis, Chick Embryo, Hyaluronidase

Footnotes

This work was supported by the Sonderforschungsbereich 47 (Virologie).

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