Summary
White Leghorn line C chicks were surgically bursectomised (Bx) in ovo to eliminate antibody production. After inoculation with infectious bronchitis virus (IBV) at 14 days after hatching, Bx chicks experienced a more severe and longer lasting infection than intact chicks. The severity and duration of clinical infection in the Bx chicks resembled that previously observed in the highly susceptible line 15 I chicks, however no increase in mortality was observed, in contrast to the high levels of mortality recorded in IBV-inoculated line 15 I chicks. After secondary challenge the degree of damage to the ciliated epithelium of the trachea was greater in the Bx chicks than in the intact chicks.
The results indicate that, although antibodies play an important role in recovery from IBV infection, other immunological factor(s) may also be involved.
Keywords: Infectious Disease, Virus Infection, Bronchitis, Antibody Production, Clinical Infection
References
- 1.Boyle JF, Weismiller DG, Holmes KV. Genetic resistance to mouse hepatitis virus correlates with absence of virus-binding activity on target tissues. J Virol. 1987;61:185–189. doi: 10.1128/jvi.61.1.185-189.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
- 2.Bumstead N, Huggins MB, Cook JKA. Genetic differences in susceptibility to a mixture of avian infectious bronchitis virus andEscherichia coli. Br Poult Sci. 1989;30:39–48. doi: 10.1080/00071668908417123. [DOI] [PubMed] [Google Scholar]
- 3.Cook JKA, Darbyshire JH, Peters RW. The use of chicken tracheal organ cultures for the isolation and assay of avian infectious bronchitis virus. Arch Virol. 1976;50:109–118. doi: 10.1007/BF01318005. [DOI] [PubMed] [Google Scholar]
- 4.Cook JKA, Smith HW, Huggins MB. Infectious bronchitis immunity: its study in chickens experimentally infected with mixtures of infectious bronchitis virus andEscherichia coli. J Gen Virol. 1986;67:1427–1434. doi: 10.1099/0022-1317-67-7-1427. [DOI] [PubMed] [Google Scholar]
- 5.Cooper MD, Cain WA, Van Alten PJ, Good RA. Development and function of the immunoglobulin producing system. Int Arch Allergy. 1969;35:242–252. [PubMed] [Google Scholar]
- 6.Davelaar FG, Kouwenhoven B. Changes in the Harderian gland of the chicken following conjunctival and intranasal infection with infectious bronchitis virus in one-and 20-day-old chickens. Avian Pathol. 1976;5:39–50. doi: 10.1080/03079457608418168. [DOI] [PubMed] [Google Scholar]
- 7.Davelaar FG, Kouwenhoven B. Influence of maternal antibodies on vaccination of chicks of different ages against infectious bronchitis. Avian Pathol. 1977;6:41–50. doi: 10.1080/03079457708418211. [DOI] [PubMed] [Google Scholar]
- 8.Gillette KG. Local antibody response to avian infectious bronchitis: virus-neutralising antibody in tracheobronchial secretions. Avian Dis. 1981;25:431–443. [PubMed] [Google Scholar]
- 9.Gomez L, Raggi LG. Local immunity to avian infectious bronchitis in tracheal organ culture. Avian Dis. 1974;18:346–368. [PubMed] [Google Scholar]
- 10.Gough RE, Alexander DJ. Comparison of duration of immunity in chickens infected with a live infectious bronchitis vaccine by three different routes. Res Vet Sci. 1979;26:329–332. [PubMed] [Google Scholar]
- 11.Herbert WJ, Kristensen F. Laboratory animal techniques for immunology. In: Weir DM, editor. Handbook of experimental immunology, vol 4, applications of immunological methods in biomedical sciences. 4th edn. Oxford: Blackwell; 1986. pp. 133·1–133·36. [Google Scholar]
- 12.Hofstad MS. Avian infectious bronchitis. In: Hofstad MS, Barnes HJ, Calnek BW, Reid WH, Yoder HW Jr, editors. Diseases of poultry. 8th edn. Ames, Iowa: The Iowa State University Press; 1984. pp. 429–443. [Google Scholar]
- 13.Mockett APA, Cook JKA. The detection of specific IgM to infectious bronchitis virus in chicken serum using an ELISA. Avian Pathol. 1986;15:437–446. doi: 10.1080/03079458608436305. [DOI] [PubMed] [Google Scholar]
- 14.Nakamura K, Cook JKA, Otsuki K, Huggins MB, Frazier JA (1991) Comparative study of respiratory lesions in two chicken lines of different susceptibility infected with infectious bronchitis virus: histology, ultrastructure and immunohistochemistry. Avian Pathol (in press) [DOI] [PubMed]
- 15.Otsuki K, Huggins MB, Cook JKA. Comparison of the susceptibility to avian infectious bronchitis virus infection of two inbred lines of White Leghorn chickens. Avian Pathol. 1990;19:467–475. doi: 10.1080/03079459008418700. [DOI] [PubMed] [Google Scholar]
- 16.Raggi LG, Lee GG. Lack of correlation between infectivity, serologic response and challenge results in immunization with an avian infectious bronchitis vaccine. J Immunol. 1965;94:538–543. [PubMed] [Google Scholar]
- 17.Reed LJ, Muench H. A simple method of estimating fifty percent end points. Am J Hyg. 1938;27:493–497. [Google Scholar]
- 18.Schat KA, Calnek BW, Fabricant J. Influence of the bursa of Fabricius on the pathogenesis of Marek's disease. Infect Immun. 1980;31:199–207. doi: 10.1128/iai.31.1.199-207.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
- 19.Smith HW, Cook JKA, Parsell ZE. The experimental infection of chickens with mixtures of infectious bronchitis virus andEscherichia coli. J Gen Virol. 1985;66:777–786. doi: 10.1099/0022-1317-66-4-777. [DOI] [PubMed] [Google Scholar]
- 20.Timms LM, Bracewell CD, Alexander DJ. Cell mediated and humoral immune response in chickens infected with avian infectious bronchitis. Br Vet J. 1980;136:349–356. doi: 10.1016/s0007-1935(17)32237-6. [DOI] [PubMed] [Google Scholar]
- 21.Winterfield RW, Fadly AM. Some characteristics of isolates of infectious bronchitis virus from commercial vaccines. Avian Dis. 1972;16:746–755. [PubMed] [Google Scholar]