Central venous catheterization in cancer patients with severe thrombocytopenia: Ultrasound-guide improves safety avoiding prophylactic platelet transfusion

Abstract

Prior research has revealed that ultrasound (US) guided central venous catheterization (CVC) is associated with a reduction in the complication rate such as pneumothorax and an improved first-pass success placing CVC in the internal jugular vein. The present study investigated if US-guided CVC, in a subset of cancer patients with severe thrombocytopenia, reduced bleeding risk and avoided prophylactic platelet transfusion. The efficacy and safety of US-guided CVC placement in cancer patients with severe thrombocytopenia was retrospectively analyzed over a period of 9 years (Dec 2000-Jan 2009), 1,660 and 207 patients with cancer underwent US-guided CVC placement into internal jugular vein respectively at the Department of Onco-Haematology, Hospital of Piacenza. The first group of patients included patients in active antitumor treatment, while the second group included patients in the palliative phase. A total of 110 (5.89%) of these 1,867 patients exhibited severe thrombocytopenia defined as platelet count ≤20x10⁹/l, and formed the basis of this study. All procedures were evaluated for bleeding complications as defined by the National Institute of Health Common Terminology Criteria for Adverse Events (CTCAE 3.0). In the subgroup of the 110 patients with severe thrombocytopenia a single needle puncture of the vein was employed in 121 of the 122 procedures (99.18%) and no attempt failures were registered. No pneumothorax, no major bleeding and no nerve and arterial puncture were reported, only one self-limiting hematoma (0.90%) at the site of CVC insertion was reported (CTCAE 3.0 grade 1). No platelet transfusions were performed in the 110 patients, pre and post CVC placement. We believe that US-guided CVC insertion procedures into the internal jugular vein makes the difference in safety, also in thrombocytopenic patients avoiding prophylactic or post procedure platelet transfusion.

Introduction

Central venous catheters (CVC) is essential in patients with cancer, and the need for intravenous access devices for the administration of cancer therapy has increased proportionally with the increasing number of patients diagnosed with cancer. CVCs include implantable central venous ports (PORTS), peripherally inserted central catheters (PICCs) and external CVCs. The percutaneous approach to the subclavian or internal jugular vein is a popular procedure for placing catheters in the superior vena cava both for short-term and long-term use. Unfortunately, central venous catheter insertion into the jugular or subclavian vein represents a risk of pneumothorax, nerve puncture and major bleeding (mechanical complications), infection and CVC-related vein thrombosis (1,2). Mechanical complications of CVC insertion without ultrasound (US) guidance, such as arterial puncture and pneumothorax, are seen in up to 21% of attempts, and up to 35% of insertion attempts are not successful (3-5). Prior researches show that the use of US-guide CVC has been associated with a reduction in complication rate and an improved first-pass success when placing CVC in the internal jugular vein (6-18). We sought to investigate within US-guidance CVC in a subset of cancer patients with severe thrombocytopenia also effects the safety, reducing bleeding complications and avoid prophylactic platelet transfusion. In this paper, we report clinical outcome on US-guided CVC insertional procedures performed in 1,867 cancer patients over a period of 9 years and we aim to evaluate the safety of US-guided CVC insertion in the internal jugular vein in a subgroup of cancer patients with severe thrombocytopenia defined as platelet count below 20x10⁹/l (19).

Patients and methods

Patients

This research was conducted based on the medical records of cancer patients to identify those who underwent US-guidance CVC placement in the internal jugular vein, and 1,867 consecutive patients were identified at the Department of Oncology-Haematology (Piacenza, Italy). All patients included in the study signed informed consent before undergoing the procedure; they signed also informed consent to utilize their clinical data for clinical research. These clinical data were abstracted from the medical records: Clinical setting: Age, diagnosis and sex. Indications for CVC insertion: Hemoglobin levels, white blood cell and platelet count in a sample obtained within 1 day prior to performing the procedure, chest radiographic findings pre- and post-CVC, pneumothorax, tube thoracotomy, hematoma, nerve puncture, hemorrhagic complication rate. Post-procedural hemoglobin values within 24 h and 3 days, packed RBC transfusion within 6 days after the procedure and platelet transfusion pre- and post-procedure were also recorded. Hemorrhagic complications were defined using the National Institutes of Health Common Terminology Criteria for Adverse Events (CTCAE 3.0) (20). Grade 1 bleeding is characterized by mild symptoms not requiring any intervention, for example, local hematoma. Grade 2 bleeding has mild symptoms requiring invasive interventions such as evacuation or aspiration. Grade 3 bleeding requires transfusion, radiologic, endoscopic or elective operative interventions. In grade 4 bleeding, life-threatening consequences make urgent intervention necessary. A total of 110 patients had severe thrombocytopenia defined as platelet count ≤ 20x10⁹/l and they form the basis of this report.

US -guided CVC

The procedure was performed using Esaote SpA equipped with two transducers between 3.5 to 7.5 MHZ, with a needle guide. The method that we commonly use is ‘the three-handed method’, as previously reported (17,18); this method requires an assistant to hold the probe, while the operator controls the needle and performs the procedure under real-time guidance, and the nurse helps the two physicians during the maneuver. The central vein was identified along its greater longitudinal axis and its relationship with other anatomical structures using Valsalva's maneuver which determines an increase of the diameter of the veins. Under US-guide in real time, a 16-gauge needle is introduced into the last portion of internal jugular vein. This vein was reached through the transducer placed at the point of insertion of the sternocleidomastoid muscle into the clavicular; the correct introduction of the needle was always confirmed by US guidance and by the easy aspiration of venous blood.

The Seldinger technique was used to place the catheter, which was advanced into the superior vena cava until insertion into right atrium.

Every procedure was scheduled in order to register patient's data, pathological diagnosis, indications for CVC insertion, type of CVC, number of attempts and early complications if any failure. Medications, CVC-related blood stream infection, symptomatic deep-vein thrombosis and CVC removal or substitution were also recorded. Within 2 h after each procedure, chest radiography and US scanning were carried out to exclude pneumothorax and to evaluate correct catheter position.

Statistical analysis

Demographic data and clinical features were analyzed using descriptive methods. Quantitative variables were summarized using mean and standard deviation. Categorical variables were summarized as counts and percentages. Baseline analysis included all enrolled patients. Statistical tests were performed with Microsoft Excel 2010 software (Microsoft Corporation).

Results

Over a period of 9 years a total of 2,187 CVC insertional procedures were applied to 1,867 cancer patients that underwent US-guided CVC catheterization in internal jugular vein at the Department of Oncology-Haematology, Hospital Guglielmo da Saliceto (Table I).

Table I.

Characteristics and results of ultrasound guided central catheter insertion in the internal jugular vein over a period of 9 years in 1,867 cancer patients.

Characteristics	Cancer patients in antitumoral treatment, n (%)	Cancer patients in palliative phase, n (%)
No. of patients total (%)	1,660 (100)	207 (100)
Male	858 (52)	106 (51.2)
Female	802 (48)	101 (48.8)
Median age years (range)	61.71 (18-85)	68 (22-86)
Type of cancer total (%)	1,660 (100)	207 (100)
Solid tumor total (%)	1,280 (77.1)	200 (96.6)
Hematological cancer total (%)	380 (22.9)	7 (2.4)
Platelet count x109/l median (range)	236 (7-510)	194 (7-254)
Total procedures of catheter insertion (total %)	1,978 (100)	209 (100)
Access with one attempt	1,948 (98.5)	206 (98.6)
Access with two attempt	30 (1.5)	3 (1.4)
Failure total (%)	18 (0.9)	0 (0)
Pneumothorax	0 (0)	0 (0)
Major bleeding	0 (0)	0 (0)
Mediastinal CVC dislocation	2 (11.1)	0 (0)
Arterial puncture	6 (33.35)	0 (0)
Vein collapse	8 (44.45)	0 (0)
Nerve puncture	0 (0)	0 (0)
No efficacious ‘eco window’	2 (11.1)	0 (0)

Of these 1,867 patients, 1,660 were treated with anticancer therapy and underwent 1,978 CVC insertional US-guided procedures with these indications: Chemotherapy delivery, transfusion, parenteral nutrition, leukapheresis, autologous and allogenic stem cell transplantation, invasive hemodynamic variables assessment and blood sampling. In this group the procedure was performed in 380 patients with hematologic malignancies and in 1,280 patients with solid tumors; the majority of patients with solid tumors had gastrointestinal cancer and the majority of patients with hematologic malignancies had lymphomas. The median platelet count at the time of CVC insertion was 236x10⁹/l (range 7-510x10⁹/l). The remaining 207 cancer patients were in advanced phase of their disease and underwent 209 US-guide CVC catheterization for parenteral nutrition and for hydration since they were in palliative phase of their trajectory of cancer history. The median platelet count at the time of CVC was 194x10⁹/l (range 7-254x10⁹/l). The procedure was performed in 7 patients with hematologic malignancies and in 200 patients with solid tumors (Table I); the majority of patients with solid tumors had gastrointestinal cancer and the majority of patients with hematologic malignancies had lymphomas (Table I). In these two series including 1,867 patients with cancer that underwent 2,187 insertional procedure, 110 patients (5.89%) had platelet count ≤20x10⁹/l (Table II).

Table II.

Results of the ultrasound-guided central vein catheterization insertional procedure in the 110 thrombocytopenic cancer patients (PLTs count ≤20 x10⁹/l; range 7-20x10⁹/l).

Results	Total n (%)	Cancer patients in antitumoral treatment, n (%)	Cancer patients in palliative phase, n(%)
Total patients	110 (100)	70 (100)	40 (100)
Total CVC insertional procedures	122 (100)	80 (100)	42 (100)
Access with one attempt	121 (99.18)	79 (98.75)	42 (100)
Access with two attempts	1 (0.82)	1 (1.25)	0 (0)
Pneumothorax	0 (0)	0 (0)	0 (0)
Major bleeding	0 (0)	0 (0)	0 (0)
Arterial puncture	0 (0)	0 (0)	0 (0)
Failure	0 (0)	0 (0)	0 (0)
Nerve puncture	0 (0)	0 (0)	0 (0)
Local hematoma	1 (0.82)	1 (1.25)	0 (0)

The median platelet count in this group was 12x10⁹/l (range 7-20 x10⁹/l), these 110 patients underwent 122 insertional procedures (Table II). In the entire group of 1,867 cancer patients that underwent 2,187 insertional CVC procedure, no pneumothorax, no major bleeding, no nerve puncture were reported, only 6 arterial puncture of 2,187 procedures (0.27%) and 4 (0.18%) of self-limiting hematomas were registered. In the subgroup of 110 patients with severe thrombocytopenia a single needle puncture of the vein was done on 121 of the 122 procedures (99.18%) and not attempts failure were registered, no pneumothorax, no major bleeding and no nerve and arterial puncture were reported, only one self-limiting hematoma (0.90%) at the site of CVC insertion was reported (CTCAE 3.0 grade 1). No platelet transfusion were done in the 110 patients, pre and post CVC placement.

Discussion

The use of central venous access devices has become an essential component of the treatment of many medical disorders. Central venous access is commonly attempted in the internal jugular vein, subclavian vein, femoral vein, or arm veins using peripherally central catheters. It is estimated that several million devices are inserted each year, facilitating many emerging therapies, including long-term chemotherapy (21). Central venous cannulation can be unsafe: The National Confidential Enquiry into perioperative deaths has reported one death resulting from a procedure-induced pneumothorax (22).

In clinical practice a challenging clinical scenario can occur when urgent treatment is necessary in patients at high risk of bleeding because of an underlying onco-hematological disorder with a very low platelet count, furthermore, such as patients with acute leukemia, in these patients US-guidance CVC can allow and immediately adequate treatment (17). Traditionally, the site of central venous access is guided by anatomical landmarks such as bony prominences, muscle surfaces, and arterial pulsations. This ‘blind’ approach to the central veins assumes anatomical uniformity, does not account for the possibility of occlusions, and depends on correct discernment of the relationship among multiple anatomical landmarks. For these reasons, the procedure is associated with a relatively high incidence of complications, related to first pass failure, arterial punctures or pneumothorax (6-18).

In comparison to standard CVC, US-guided CVC is more feasible and offers various advantages (ease of vein identification, a shorter duration of the procedure) which, together with a higher rate of success and decreased incidence of complications, make the latter preferable to the former, especially in high-risk patients.

Patients with a low platelet count, above all patients with cancer, often require the insertion of CVC, and low platelet count may be considered a relative contraindication to the insertion of a CVC due to the risk of bleeding (23-28). Current practice in many countries is correct thrombocytopenia with platelet transfusion prior to CVC insertion, with the objective to mitigate the risk of serious pre or post procedural bleeding (23). The platelet count threshold recommended prior to CVC insertion varies significantly from country to country. In the UK the current threshold is 50x10⁹/l (24), in Belgium the threshold is 30x10⁹/l (25), in the United States (US) the threshold is 20x10⁹/l (26) and in Germany the threshold is 10x10⁹/l unless there are risk factors for bleeding (27). However it must be emphasized that platelet transfusion may be associated with adverse event such as rigors, fever, urticaria; serious sequelae are rare and include anaphylaxis, that can be life threatening (23,28). In addition the requirement to administer platelet transfusions to correct thrombocytopenia prior to central line insertion may additionally delay the start of treatment, which may be time-critical in patient with acute leukemia, or in patient in intensive care.

Above all it remains unclear whether platelet transfusion in thrombocytopenic non-bleeding patients, despite improving the platelet count, reduce the incidence of clinically-important bleeding or improve other meaningful patient-oriented outcomes, such as mortality (23,28). Patients may therefore be exposed to the risks of a platelet transfusion without any obvious clinical benefit. In a retrospective analysis, that included 193 consecutive adult patients receiving 604 CVC insertions, 93 cases had platelet count ≤ 20x10⁹/l: The majority of bleedings were grade 1, and 4% grade 2, while no moderate, and no severe bleedings (grade 3 or 4) were reported (29) it must be emphasized, that in this series the number of pneumothorax was 6 on 604 (1%), but in this series the CVCs were inserted without US-guidance. Our data demonstrate that in a large number of cancer patients: 1,867 underwent 2,187 US-guided insertional procedures no pneumothorax were reported, in addition, between the 110 thrombocytopenic cancer patients, that underwent US-guided CVC insertion, only one patient showed a local mild hematoma, without clinical significance, no patients of this series received prophylactic platelet transfusion to prevent bleeding of CVC insertional procedure. Other invasive procedures such as thoracentesis in thrombocytopenic cancer patients may be safety done under US-guidance, without prophylactic platelet transfusion as recently reported by our group (30), and other report confirm that US-guided CVC is a safe and highly successful modality also in liver disease patients with damaged coagulation (31). A prospective, randomized controlled trial powered to test the hypothesis of whether omitting forgoing platelet transfusion prior to US-guided CVC insertion leads to an equal occurrence of clinically relevant bleeding complications in patients with thrombocytopenia is currently recruiting (32). In conclusion, we agree with the review article reported in this journal that stated it is strongly recommended to use real-time US-guidance for central venous access (33) and we believe that US-guidance of CVC insertion procedures makes the difference in safety, also in thrombocytopenic patients avoiding prophylactic platelet transfusion.

Funding

No funding was received.

Availability of data and materials

The datasets generated and/or analyzed during the present study are not publicly available due to the hospital's privacy regulations but are available from the corresponding author on reasonable request.

Authors' contributions

LC designed the study, performed the experiments and wrote the paper. CC collected and analyzed the data, and wrote the paper. CDN analyzed the data and wrote the paper. EO, IT and MA performed the experiments and wrote the paper. All authors read and approved the final manuscript.

Ethics approval consent to participate

All of the patients included in the present retrospective study previously signed informed consent before undergoing the procedure and provided informed consent for the use of their data for clinical research.

Patient consent for publication

Patients gave their written informed consent prior to their inclusion in this report.

Competing interests

The authors declare that they have no competing interests.