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. 1989;18(3):374–387. doi: 10.1007/BF01062362

Effects of T-2 mycotoxin on gastrointestinal tissues: A Review ofin vivo andin vitro models

Phletus P Williams 1
PMCID: PMC7087545  PMID: 2658861

Abstract

T-2 mycotoxin, a trichothecene, is the principal toxic component ofFusarium sp. Agricultural products and food are frequently contaminated with this toxin. Various animal models have been used to determine its metabolic fate, rate of excretion, and distribution. A modulation effect on cell-mediated immunity and alterations in gastrointestinal propulsion have been demonstrated. The toxin has been shown to produce some similar pathologic alterations in various animal species studied. The consistent alteration appears to mainly affect mitotic cells of the gastrointestinal tract and the lymphoid system. A host of bioassay systems are now being used as alternative methods to the use of animals for testing of the mycotoxin. These tests may accurately assess and define the role of the subject-toxin interactions following consumption of T-2 mycotoxin contaminated food sources.

T-2 mycotoxin, as observed above within vivo andin vitro models, promotes a chemically-induced change in structure and function of affected gastrointestinal cells from a transient and reversible aberration in a single enzymatic reaction to cell death. Regardless of the end point measured, the toxic response brought about in cells appears to involve the interactions of virtually all subcellular processes—membrane transport and permeability, chemical metabolism, DNA function, and energy production/expenditure—as cells attempt to maintain their functional integrity while disposing of the toxicant. The variation in the quality of the toxic response with dose suggests that more cellular processes are perturbed as the chemical dose is increased.

Keywords: Mitotic Cell, Trichothecene, Metabolic Fate, Toxic Response, Pathologic Alteration

References

  1. Abbas HK, Shier WT, Mirocha CJ. Sensitivity of cultured human and mouse fibroblasts to trichothecenes. J Assoc Offic Anal Chem. 1984;67:607–610. [PubMed] [Google Scholar]
  2. Acosta D, Sorensen EMB, Anuforo DC, Mitchell DB, Ramos K, Santone K, Smith MA. Anin vitro approach to the study of target organ toxicity of drugs and chemicals. In Vitro Cell Dev Biol. 1985;21:495–504. doi: 10.1007/BF02620841. [DOI] [PubMed] [Google Scholar]
  3. Agrelo CE, Schoental R. Synthesis of DNA in human fibroblast treated with T-2 toxin and HT-2 (the trichothecene metabolites ofFusarium species) and the species of hydroxyurea. Toxicol Lett. 1980;5:155–160. doi: 10.1016/0378-4274(80)90166-6. [DOI] [PubMed] [Google Scholar]
  4. Bamburg JR. Biological and biochemical actions of trichothecene mycotoxins. In: Hahn FE, editor. Progress in Molecular and Subcellular Biology. Berlin: Springer-Verlag; 1983. pp. 41–110. [Google Scholar]
  5. Bamburg JR, Strong FM. 12,13-Epoxy-trichothecenes. In: Kadis S, Ciegler A, Ajl SJ, editors. Microbial Toxins, A Comprehensive Treatise, Algal and Fungal Toxin. Vol 7. New York: Academic Press; 1971. pp. 207–292. [Google Scholar]
  6. Bauer J, Gareis M, Enders C, Gedek B. Comparative studies on the metabolism of diacetoxyscirpenol and zearalenone in pigs. Book of Abstracts, 6. Intern. IUPAC Symp. on Mycotoxins and Phycotoxins. 1985;22:43. [Google Scholar]
  7. Beach RS, Gershivin ME, Makishima RK, Hurley LS. Impaired immunologic ontogeny in postnatal zinc deprivation. J Nutr. 1980;110:805–815. doi: 10.1093/jn/110.4.805. [DOI] [PubMed] [Google Scholar]
  8. Beasly VR, Swanson SP, Corley RA, Buck WB, Koritz GD, Burmeister HR. Pharmacokinetics of the trichothecene mycotoxin T-2 toxin, in swine and cattle. Toxicon. 1986;24:13–23. doi: 10.1016/0041-0101(86)90161-3. [DOI] [PubMed] [Google Scholar]
  9. Bianchi V. Nucleotide pool unbalance induced in cultured cells by treatments with different chemicals. Toxicology. 1982;25:13–18. doi: 10.1016/0300-483x(82)90080-4. [DOI] [PubMed] [Google Scholar]
  10. Bitay Z, Glavits R, Sandor G, Gabriella S, Balazs K. A case of T-2 mycotoxicosis in broiler chicks. Magyar Allatorvosok Lapja. 1981;36(7):491–495. [Google Scholar]
  11. Bodon L, Zoldag L. Cytotoxicity studies on T2 fusariotoxin. Acta Vet Acad Sci Hung. 1974;24:451–455. [PubMed] [Google Scholar]
  12. Brennecke LH, Neufeld HA. Pathologic effects and LD50 doses of T2 toxin in rats by intramuscular, subcutaneous, and intraperitoneal routes of administration. Fed Proc. 1982;41:924. [Google Scholar]
  13. Browning TH, Trier JS. Organ culture of mucosal biopsies of human small intestine. J Clin Invest. 1969;48:1423–1432. doi: 10.1172/JCI106108. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Buening GM, Mann DD, Hook B, Osweiler GD. The effect of T-2 toxin on the bovine immune system: cellular factors. Vet Immunol Immunopathol. 1982;3:411–417. doi: 10.1016/0165-2427(82)90023-x. [DOI] [PubMed] [Google Scholar]
  15. Cannon M, Smith KE, Carter CJ. Prevention by ribosome bound nascent polyphenylalanine chains of the functional interaction of T-2 toxin with its receptor site. Biochem J. 1976;156:289–296. doi: 10.1042/bj1560289. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Carlton WC, Szezech GM. Mycotoxicoses in laboratory animals. In: Wyllie TD, Morehouse LG, editors. Mycotoxic Fungi, Mycotoxins, Mycotoxicoses: An Encyclopedic Handbook. Vol 2. New York: Marcel Dekker; 1978. pp. 333–462. [Google Scholar]
  17. Carter CC, Cannon M. Structural requirement for the inhibitory action of 12,13 epoxy trichothecenes on protein synthesis in eukaryotes. Biochem J. 1977;166:399–409. doi: 10.1042/bj1660399. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Chanin BE, Hamilton DL, Hancock DS, Schiefer HB. Biointeraction of dietary T-2 toxin and zinc in mice. Can J Physiol Pharmacol. 1984;62:1320–1326. doi: 10.1139/y84-221. [DOI] [PubMed] [Google Scholar]
  19. Chi MS, Robison TS, Mirocha CJ, Swanson SP, Shimoda W. Excretion and tissue distribution of radioactivity from tritium labeled T-2 toxin in chicks. Toxicol Appl Pharmacol. 1978;45:391–402. doi: 10.1016/0041-008x(78)90103-5. [DOI] [PubMed] [Google Scholar]
  20. Ciegler A. Trichothecenes, occurrence and toxicoses. J Food Protect. 1979;41:399–403. doi: 10.4315/0362-028X-41.5.399. [DOI] [PubMed] [Google Scholar]
  21. Cole RJ, Cox RH. In: Handbook of Toxic Fungal Metabolites. Cole RJ, Cox RH, editors. New York: Academic Press; 1981. p. 1850. [Google Scholar]
  22. Conner MW, De Camargo J, Punyarit P, Riengropitak S, Rogers AE, Newberne PM. Toxicity of anguidine in mice. Fund Appl Toxicol. 1986;7:153–164. doi: 10.1016/0272-0590(86)90209-5. [DOI] [PubMed] [Google Scholar]
  23. Conrady-Lorch S, Strugala G, Fichtl B. Investigations on the intestinal metabolism of T-2 toxin a major trichothecene mycotoxin. 27th Spring Mtg German Pharmacological Soc Naunyn-Schmiedeberg's Arch Pharmacol. 1986;332(Suppl):R24. [Google Scholar]
  24. Corley RA, Swanson SP, Buck WB. Glucuronide conjugates of T-2 toxin and metabolites in swine bile and urine. J Agric Food Chem. 1985;33:1085–1089. [Google Scholar]
  25. Corley RA, Swanson SP, Gullo GJ, Johnson L, Beasely VR, Buck WB. Disposition of T-2 toxin, a trichothecene mycotoxin, in intravascularly dosed swine. J Agric Food Chem. 1986;34:868–875. [Google Scholar]
  26. Corrier DE, Ziprin RL, Mollenhauer HH. Modulation of cell-mediated resistance to listeriosis in mice given T-2 toxin. Toxicol Appl Pharmacol. 1987;89:323–331. doi: 10.1016/0041-008x(87)90152-9. [DOI] [PubMed] [Google Scholar]
  27. Cousins RJ. Mechanism of zinc absorption. In: Prasad AS, editor. Current topics in nutrition and disease. Vol. 6. New York: Alan R. Liss Inc.; 1982. pp. 117–128. [Google Scholar]
  28. Cundliffe E, Cannon M, Davies J. Mechanism of inhibition of eukaryotic protein synthesis by trichothecene group (fungal toxins) Proc Natl Acad Sci. 1974;71:30–34. doi: 10.1073/pnas.71.1.30. [DOI] [PMC free article] [PubMed] [Google Scholar]
  29. Dagani R. Alternative methods could cut animal use in toxicity tests. Chem Eng News. 1983;61:7–13. [Google Scholar]
  30. De Nicola DB, Rebar AH, Carlton WW. T2 toxin mycotoxicosis in the guinea-pig. Food Cosmet Toxicol. 1978;16:601–609. doi: 10.1016/s0015-6264(78)80230-2. [DOI] [PubMed] [Google Scholar]
  31. Di Ninno VL, Penman D, Bhatti AR, Erhardt NP, Lockwood PA. In vitro toxicity of T-2 toxin in mouse lymphoid cells. J Gen Microbiol. 1985;131:1833–1835. doi: 10.1099/00221287-131-7-1833. [DOI] [PubMed] [Google Scholar]
  32. Doebler JA, Wall TJ, Moore RA, Martin LJ, Shih TM, Anthony A. Soman toxication in hypoxia acclimated rats: Alterations in brain neuronal RNA and survival. Neurochem Res. 1984;9:1239–1252. doi: 10.1007/BF00973037. [DOI] [PubMed] [Google Scholar]
  33. Eastwood GL, Trier JS. Epithelial cell renewal in cultured rectal biopsies in ulcerative colitis. Gastroenterology. 1973;64:383–390. [PubMed] [Google Scholar]
  34. Eichholz A. Structural and functional organization of the brush border of intestinal epithelial cells. III. Enzymic activities and chemical composition of various fractions of tris-disrupted brush borders. Biochim Biophys Acta. 1967;135:475–482. doi: 10.1016/0005-2736(67)90037-5. [DOI] [PubMed] [Google Scholar]
  35. Enders GA, Delius M, Ballhaus S, Brendel W. Role of Peyer's patch in the intestinal immune response lo cholera toxin in enterically immunized rats. Infect Immun. 1987;55:1997–1999. doi: 10.1128/iai.55.9.1997-1999.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  36. Fairhurst S, Marrs TC, Parker HC, Scawin JW, Swanston DW. Acute toxicity of T-2 toxin in rats, mice, guinea pigs and pigeons. II. Variations in surface architecture found by scanning electron microscopy. Toxicology. 1987;43:31–50. doi: 10.1016/0300-483x(87)90072-2. [DOI] [PubMed] [Google Scholar]
  37. Fargeas MJ, Fioramonti J, Bueno L. Prostaglandins E2: A neuromodulator in the central control of gastrointestinal motility and feeding behavior by calcitonin. Science. 1984;225:1050–1052. doi: 10.1126/science.6591429. [DOI] [PubMed] [Google Scholar]
  38. Fenoglio CM, Richart RM, Kaye GI. Comparative electron-microscopic features of normal, hyperplastic, and adenomatous human colonic epithelium. Gastroenterology. 1975;69:100–109. [PubMed] [Google Scholar]
  39. Fioramonti J, Fargeas MJ, Bueno L. Action of T-2 toxin on gastrointestinal transit in mice protective effect of an argillaceous compound. Toxicol Lett. 1987;36:227–232. doi: 10.1016/0378-4274(87)90190-1. [DOI] [PubMed] [Google Scholar]
  40. Fraker PJ, Haas SM, Luecke RW. Effect of zinc deficiency on the immune response of the young adult A/J mouse. J Nutr. 1977;107:1889–1895. doi: 10.1093/jn/107.10.1889. [DOI] [PubMed] [Google Scholar]
  41. Fraker PJ, Zwickl CM, Luecke RW. Delayed type hypersensitivity in zinc deficient adult mice: impairment and restoration of responsivity to dinitrofluorobenzene. J Nutr. 1982;112:309–313. doi: 10.1093/jn/112.2.309. [DOI] [PubMed] [Google Scholar]
  42. Friend SCE, Shiefer HB, Babiuk LA. The effect of T-2 toxin on acute herpes simplex virus type-1 infection in mice. Vet Pathol. 1983;20:737–760. doi: 10.1177/030098588302000609. [DOI] [PubMed] [Google Scholar]
  43. Gareis M, Bauer J, Gedek B. Fusarien-toxine in Futtermittein. Nachweis und Vorkommen von Trichothecenen. Tierarztl Prax Suppl. 1985;1:8–19. [PubMed] [Google Scholar]
  44. Gareis M, Hashem A, Bauer J, Gedek B. Identification of glucuronide metabolites of T-2 toxin and diacetoxyscirpenol in the bile of isolated perfused rat liver. Toxicol Appl Pharmacol. 1986;84:168–172. doi: 10.1016/0041-008x(86)90424-2. [DOI] [PubMed] [Google Scholar]
  45. Gentry PA, Cooper ML. Effect ofFusarium T-2 toxin on hematological and biochemical parameters in rabbit. Can J Comp Med. 1981;45:400–405. [PMC free article] [PubMed] [Google Scholar]
  46. Gyongyossy-Issa MIC, Khanna V, Khachatourian GG. Changes induced by T-2 toxin in the erythrocyte membrane. Fd Chem Toxicol. 1986;24:311–317. doi: 10.1016/0278-6915(86)90008-6. [DOI] [PubMed] [Google Scholar]
  47. Goodwin W, Haas CD, Fabian C, Heller-Bettinger I, Hoogstraten B. Phase I evaluation of aguidine (diacetoxyscirpenol, NSC-141537) Cancer. 1978;42:23–26. doi: 10.1002/1097-0142(197807)42:1<23::aid-cncr2820420104>3.0.co;2-i. [DOI] [PubMed] [Google Scholar]
  48. Grisham JW, Smith GJ. Predictive and mechanistic evaluation of toxic responses in mammalian cell culture systems. Pharmacol Rev. 1984;36:151S–171S. [PubMed] [Google Scholar]
  49. Gustafson DP. Pseudorabies. In: Leman AD, Straw B, Glock RD, Mengeling WL, Penny RHC, Scholl E, editors. Diseases of Swine. 6th Ed. Ames, IA: Iowa State University Press; 1986. pp. 274–289. [Google Scholar]
  50. Hall MR, Williams PP, Rimler RB. A toxin fromPasteurella multocida serogroup D enhances swine herpesvirus 1 replication/lethalityin vitro andin vivo. Curr Microbiol. 1987;15:277–281. [Google Scholar]
  51. Hayes AW. Mycotoxins: a review of biological effects and their role in human diseases. Clin Toxicol. 1980;17:45–83. doi: 10.3109/15563658008985070. [DOI] [PubMed] [Google Scholar]
  52. Hayes MA. PhD. thesis. Saskatoon, Sask, Canada: University of Saskatchewan; 1979. Morphological and toxicological studies on experimental T-2 mycotoxicosis. [Google Scholar]
  53. Hayes MA, Bellamy JEC, Schiefer HB. Subacute toxicity of dietary T2 toxin in mice: Morphological and hematological effects. Can J Comp Med. 1980;44:203–218. [PMC free article] [PubMed] [Google Scholar]
  54. Hayes MA, Schiefer HB. Subacute toxicity of dietary T-2 toxin in mice: Influence of protein nutrition. Can J Comp Med. 1980;44:219–228. [PMC free article] [PubMed] [Google Scholar]
  55. Hoerr FJ, Carlton WW, Yagen B, Joffe AZ. Mycotoxicosis caused by either T-2 toxin or diacetoxyscirpenol in the diet of broiler chickens. Fund Appl Toxicol. 1982;2:121–124. doi: 10.1016/s0272-0590(82)80092-4. [DOI] [PubMed] [Google Scholar]
  56. Holden C. Unequivocal evidence of Soviet toxin use. Science. 1982;216:154–155. doi: 10.1126/science.7063875. [DOI] [PubMed] [Google Scholar]
  57. Hsia CC, Tzian BL, Harris CC. Proliferative and cytotoxic effects ofFusarium T2 toxin on cultured human fetal esophagus. Carcinogenesis. 1983;4:1101–1107. doi: 10.1093/carcin/4.9.1101. [DOI] [PubMed] [Google Scholar]
  58. Hsu IC, Smalley EB, Strong FM, Ribelin WE. Identification of T-2 toxin in mouldy corn associated with lethal toxicosis in cattle. Appl Microbiol. 1972;24:684–690. doi: 10.1128/am.24.5.684-690.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  59. Ishii K, Hara M, Ueno Y. A proposal for deepoxydation and reepoxydation cycle of epoxy-trichothecene mycotoxin T-2 toxin. Toxicol Lett. 1986;31(Suppl):178. [Google Scholar]
  60. Jagadeesen V, Rukmini C, Vijayaraghavan M, Tulpule PG. Immune studies with T2 toxin: Effects of feeding and withdrawal in monkeys. Food Chem Toxicol. 1982;20:83–87. doi: 10.1016/s0278-6915(82)80014-8. [DOI] [PubMed] [Google Scholar]
  61. Joffe AZ. Alimentary toxic aleukia. In: Kadis S, Ciegler A, Ajl SJ, editors. Microbial Toxins. Vol 7. New York: Academic Press; 1971. pp. 139–189. [Google Scholar]
  62. Kagnoff MF, Donaldson RM, Trier JS. Organ culture of rabbit small intestine: prolongedin vitro steady state proteinsynthesis and secretion and secretory IgA secretion. Gastroenterology. 1972;63:541–551. [PubMed] [Google Scholar]
  63. Kemppainen BW, Riley RT, Pace JG. Penetration of {3H}T-2 toxin through excised human and guinea-pig skin during exposure to {3H}T-2 toxin absorbed to corn dust. Food Chem Toxicol. 1972;22:893–896. doi: 10.1016/0278-6915(84)90172-8. [DOI] [PubMed] [Google Scholar]
  64. Kosuri NR, Grove MD, Yates SG, Tallent WH, Ellis JJ, Wolff IA, Nicholos RE. Response of cattle to mycotoxins ofFusarium tricinctum, isolated from corn and fescue. J Am Vet Med Assoc. 1970;157:938–940. [PubMed] [Google Scholar]
  65. Kotsonis FN, Smalley EB, Ellison RA, Gale CM. Feed refusal factors in pure cultures ofFusarium roseum “graminareum”. Appl Microbiol. 1975;30:362–368. doi: 10.1128/am.30.3.362-368.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  66. Kurtz HJ. Comparative pathologic changes in trichothecene toxicosis. In: Richard JL, Thurston JR, editors. Diagnosis of Mycotoxicoses. Boston, MA: Martinus Nijhoff Pub; 1986. pp. 191–194. [Google Scholar]
  67. Kurtz HJ. Scirpenol (Trichothecenes) In: Howard JL, editor. Current Veterinary Therapy-Food Animal Practice. Philadelphia, PA.: WB Saunders; 1981. pp. 410–411. [Google Scholar]
  68. LaFarge-Frayssinet C, DeCloitre F, Moussell S, Martin M, Frayssinet C. Induction of DNA single-strand breaks by T-2 toxin, a trichothecene metabolite ofFusarium. Mutat Res. 1981;88:115–123. doi: 10.1016/0165-1218(81)90010-0. [DOI] [PubMed] [Google Scholar]
  69. Lundeen GR, Poppenga RH, Beasley VR, Buck WB, Tranquilli WJ, Lambert RJ. Systemic distribution of blood flow during T-2 toxin induced shock in swine. Fund Appl Toxicol. 1986;7:309–323. doi: 10.1016/0272-0590(86)90161-2. [DOI] [PubMed] [Google Scholar]
  70. Lutsky IL, Mor N. Alimentary toxic aleukia (Septic angina, Endemic Panmyelotoxicosis. Alimentary hemorrhagic aleukia) Am J Pathol. 1981;104:189–191. [PMC free article] [PubMed] [Google Scholar]
  71. Lutsky I, Mor N, Yagen B, Joffe AZ. The role of T-2 toxin in experimental alimentary toxic aleukia: A toxicity study in cats. Toxicol Appl Pharmacol. 1978;43:111–124. doi: 10.1016/s0041-008x(78)80036-2. [DOI] [PubMed] [Google Scholar]
  72. Marasas WFO, Bamburg JR, Smalley EB, Strong FM, Ragland WL, Dergurse PE. Toxic effects on trout, rats and mice of T-2 toxin produced by the fungusFusarium tricinctum (Cd) Synd ed Hans. Toxicol Appl Pharmacol. 1969;15:471–482. doi: 10.1016/0041-008x(69)90045-3. [DOI] [PubMed] [Google Scholar]
  73. Marasas WFO, Nelson PE. In: Mycotoxicology. Marasas WFO, Nelson PE, editors. University Park, Pa.: Penn State Univ Press; 1987. pp. 43–44. [Google Scholar]
  74. Marrs TC, Edginton JAG, Price PN, Upshall DG. Acute toxicity of T2 mycotoxin to the guinea pig by inhalation and subcutaneous routes. Br J Exp Pathol. 1986;67:259–268. [PMC free article] [PubMed] [Google Scholar]
  75. Martin LJ, Doebler JA, Anthony A. Scanning cytophotometric analysis of brain neuronal nuclear chromatin changes in acute T-2 toxin-treated rats. Toxicol Appl Pharmacol. 1986;85:207–214. doi: 10.1016/0041-008x(86)90114-6. [DOI] [PubMed] [Google Scholar]
  76. Masuko H, Ueno Y, Otakawa M, Yaginuma K. The enhancing effect of T-2 toxin on delayed hypersensitivity in mice. Jpn J Med Sci Biol. 1977;30:159–163. doi: 10.7883/yoken1952.30.159. [DOI] [PubMed] [Google Scholar]
  77. Matsumoto H, Ito T, Ueno Y. Toxicological approaches to the metabolites of fusaria. XII. Fate and distribution of T-2 toxin in mice. Jpn J Exp Med. 1978;48:393–399. [PubMed] [Google Scholar]
  78. Matusoka Y, Kubota K. Studies on mechanisms of diarrhea induced by fusarenon-X, a trichothecene mycotoxin fromFusarium species. Toxicol Appl Pharmacol. 1981;57:293–301. doi: 10.1016/0041-008x(81)90230-1. [DOI] [PubMed] [Google Scholar]
  79. Matusoka Y, Kubota K. Studies of mechanisms of diarrhea induced by fusarenon-x, a trichothecene mycotoxin fromFusarium species: The effects of fusarenon-x and various carthartics on the digestion and the absorption in the mouse intestine. JPharm Soc Jpn. 1985;105:77–82. doi: 10.1248/yakushi1947.105.1_77. [DOI] [PubMed] [Google Scholar]
  80. Matsuoka Y, Kubota K, Ueno Y. General pharmacological studies of fusarenon-X, a trichothecene mycotoxin fromFusarium species. Toxicol Appl Pharmacol. 1979;50:87–94. doi: 10.1016/0041-008x(79)90495-2. [DOI] [PubMed] [Google Scholar]
  81. Mayer CF. Endemic panmyelotoxicosis in the Russian grain belt. Part one: The clinical aspects of alimentary toxic aleukia (ATA). A comprehensive review. M I Surg. 1953;113:173–189. [PubMed] [Google Scholar]
  82. McLaughlin CS, Vaugh MH, Campbell IM, Wei CM, Stafford ME, Hansen BS. In: Mycotoxins in Human and Animal Health. Rodricks V, Hesseltine CW, Mehlman MA, editors. Forest Smith, IL: Pathotox Pub Inc; 1977. pp. 264–273. [Google Scholar]
  83. Melmed RN, Ishai-Michaeli R, Yagen B. Differential inhibition by T-2 toxin of total protein, DNA and isoprenoid synthesis in the culture macrophage cell line J 774. Biochem Pharmacol. 1985;34:2809–2812. doi: 10.1016/0006-2952(85)90583-0. [DOI] [PubMed] [Google Scholar]
  84. Miranda CL, Henderson MC, Reed RL, Schmitz JA, Buhler DR. Protective action of zinc aganst pyrrolizidine alkaloid-induced hepatotoxicity in rats. J Toxicol Environ Health. 1982;9:359–366. doi: 10.1080/15287398209530169. [DOI] [PubMed] [Google Scholar]
  85. Mirocha CJ. Effect of trichothecene mycotoxins on farm animals. In: Ueno Y, editor. Trichothecenes: Chemical, Biological and Toxicological Aspects. Amsterdam: Kodansha LTD, Tokyo and Elsevier Science Pub BV; 1983. pp. 177–193. [Google Scholar]
  86. Mirocha CJ, Pawlosky RA, Chatterjee K, Watson S, Hayes W. Analysis forFusarium toxins in various samples implicated in biological warfare in Southeast Asia. J Assoc Offic Anal Chem. 1983;66:1485–1499. [PubMed] [Google Scholar]
  87. Mirocha CJ, Watson SA, Hayes AW (1982) Occurrence of trichothecenes in samples from Southeast Asia associated with “yellow rain.” Proc 5th International IUPAC Symposium on Mycotoxins and Phycotoxins. Vienna, Sept, pp 130–133
  88. Niyo KA, Richard JL, Niyo Y, Tiffany LH (1988a) Effects of T-2 mycotoxin ingestion on phagocytosis ofAspergillus fumigatus Fresenius conidia by rabbit alveolar macrophages and on hematologic, serum biochemical and pathologic changes in rabbits. Am J Vet Res (in press) [PubMed]
  89. - (1988b) Pathologic, hematologic and serologic changes in rabbits given T-2 mycotoxin orally and exposed to aerosols ofAspergillus fumigatus Fresenius conidia. Am J Vet Res (in press) [PubMed]
  90. Oldham JW, Allred LE, Milo GE, Kindig O, Capen CC. The toxicological evaluation of the mycotoxins T-2 and T-2 tetraol using normal human fibroblastsin vitro. Toxicol Appl Pharmacol. 1980;52:159–168. doi: 10.1016/0041-008x(80)90255-0. [DOI] [PubMed] [Google Scholar]
  91. Otokawa M. Immunological disorders. In: Ueno Y, editor. Trichothecenes: Chemical, Biological and Toxicological Aspects. Amsterdam: Kodansha LTD, Tokyo and Elsevier Science Pub BV; 1983. pp. 163–170. [Google Scholar]
  92. Pace JG. Effect of T2 mycotoxin on rat liver mitochondria electron transport system. Toxicon. 1983;21:675–680. doi: 10.1016/0041-0101(83)90273-8. [DOI] [PubMed] [Google Scholar]
  93. Pace JG, Watts MR, Burrows EP, Dinterman RE, Maison C, Hauer EC, Wannemacher RW., Jr Fate and distribution of tritium-labeled T-2 mycotoxin in guinea pigs. Toxicol Appl Pharmacol. 1985;80:377–385. doi: 10.1016/0041-008x(85)90382-5. [DOI] [PubMed] [Google Scholar]
  94. Palyusik M, Koplik-Kovacs E. Effect on laying geese of feeds containing the fusariotoxins T2 and F2. Acta Vet Acad Sci Hung. 1975;25(4):363–368. [PubMed] [Google Scholar]
  95. Pang VF, Felsburg PJ, Beasley VR, Buck WB, Haschek WM. The toxicity of T-2 toxin in swine following topical application. II. Effects on hematology, serum biochemistry and immune response. Fund Appl Toxicol. 1987;9:50–59. doi: 10.1016/0272-0590(87)90153-9. [DOI] [PubMed] [Google Scholar]
  96. Pang VF, Lorenzana RM, Beasley VR, Buck WB, Haschek WM. Experimental T-2 toxicosis in swine III. Morphologic changes following intravascular administration of T-2 toxin. Fund Appl Toxicol. 1987;8:298–309. doi: 10.1016/0272-0590(87)90080-7. [DOI] [PubMed] [Google Scholar]
  97. Pang VF, Swanson SP, Beasley VR, Buck WB, Haschek WM. The toxicity of T-2 toxin in swine following topical application. I. Clinical signs, pathology and residue concentrations. Fund Appl Toxicol. 1987;9:41–49. doi: 10.1016/0272-0590(87)90152-7. [DOI] [PubMed] [Google Scholar]
  98. Perlman D, Leummis WL, Geiersbach HJ. Differential agar-diffusion bioassay for cytotoxic substances. J Pharm Sci. 1969;58:633. doi: 10.1002/jps.2600580530. [DOI] [PubMed] [Google Scholar]
  99. Petrie L, Robb J, Stewart AF. The identification of T-2 toxin and its association with haemorrhagic syndrome in cattle. Vet Rec. 1977;101:326–327. doi: 10.1136/vr.101.16.326. [DOI] [PubMed] [Google Scholar]
  100. Pier AC, Cysewski SJ, Richard JL, Baetz AK. Experimental mycotoxicoses in calves with aflatoxin, ochratoxin, rubratoxin and T-2 toxin. Proc US Animal Health Assoc. 1976;80:130–148. [PubMed] [Google Scholar]
  101. Pokrovskii AA, Kravchenko LV, Tutelyan VA. Effect of aflatoxin on rat liver lysosomes. Toxicon. 1972;10:25–30. doi: 10.1016/0041-0101(72)90086-4. [DOI] [PubMed] [Google Scholar]
  102. Pokrovskii AA, Tutelyan VA, Olifson LE, Kravchenko LV. Action of the mycotoxin ofFusarium sporotrichiella v sporotrichioides on lysosomal membranes. Bull Exp Biol Med. 1974;77:755–757. doi: 10.1007/BF00799310. [DOI] [PubMed] [Google Scholar]
  103. Rafai P, Tuboly S. Effect of T-2 toxin on adrenocortical function and immune response in growing pigs. Zentralbl Veterinarmed Reihe B. 1982;29:558–565. doi: 10.1111/j.1439-0450.1982.tb01255.x. [DOI] [PubMed] [Google Scholar]
  104. Resta S, Luby JP, Rosenfeld CR, Siegel JD. Isolation and propagation of a human enteric coronavirus. Science. 1985;229:978–981. doi: 10.1126/science.2992091. [DOI] [PubMed] [Google Scholar]
  105. Richard JL, Cysewski SJ, Pier AC, et al. Comparison of effects of dietary T-2 toxin on growth, immunogenic organs, antibody formation and pathologic changes in turkeys and chickens. Am J Vet Res. 1978;39:1674–1679. [PubMed] [Google Scholar]
  106. Robert TR, Rosen JD. Presence of fourFusarium mycotoxins and synthetic material in “yellow rain”. Biomed Mass Spectrom. 1982;9:443–450. doi: 10.1002/bms.1200091007. [DOI] [PubMed] [Google Scholar]
  107. Robinson JP. Chemical warfare: Some events of the past year and their implications. Pugwash Newslett. 1982;19:157–164. [Google Scholar]
  108. Robison TS, Mirocha CJ, Kurtz HJ, Behrens JC, Chi MS, Weaver GA, Nystrom SD. Transmission of T-2 toxin into bovine and porcine milk. J Dairy Sci. 1979;62:637–641. doi: 10.3168/jds.s0022-0302(79)83301-9. [DOI] [PubMed] [Google Scholar]
  109. Robison TS, Mirocha CJ, Kurtz HJ, Behrens JC, Weaver GA, Chi MS. Distribution of tritium-labeied T-2 toxin in swine. J Agric Food Chem. 1979;27:1411–1413. doi: 10.1021/jf60226a022. [DOI] [PubMed] [Google Scholar]
  110. Rosenstein Y, Lafarge-Frayssinet C. Inhibitory effect ofFusarium T-2 toxin on lymphoid DNA and protein synthesis. Toxicol Appl Pharmacol. 1983;70:283–288. doi: 10.1016/0041-008x(83)90104-7. [DOI] [PubMed] [Google Scholar]
  111. Rosenstein Y, LaFarge-Frayssinet C, Lespinats G, Loisillier F, Lafont P, Frayssinet C. Immunosuppressive activity ofFusarium toxins: effects of antibody synthesis and skin grafts of crude extracts, T-2 toxin and diacetoxyscirpenol. Immunology. 1979;36:111. [PMC free article] [PubMed] [Google Scholar]
  112. Roush WR, Marietta MA, Russo-Rodriguez S, Recchia J. Trichothecene metabolism studies: Isolation and structure determination of 15-acetyl-3a-(1′b-D-glucopyranosiduronyl)-scirpen-3,4b,15-triol. J Am Chem Soc. 1985;107:3354–3355. [Google Scholar]
  113. Rukimini C, Prasad JS, Rao K. Effect of feeding T-2 toxin to rats and monkeys. Food Cosmet Toxicol. 1980;18:267–269. doi: 10.1016/0015-6264(80)90106-6. [DOI] [PubMed] [Google Scholar]
  114. Ruwart MJ, Rush BD. The effects of PGF2a, PGE2 and 16,16 dimethyl PGE2 on gastric emptying and small intestinal transit in rat. Prostaglandins. 1984;28:915–928. doi: 10.1016/0090-6980(84)90044-3. [DOI] [PubMed] [Google Scholar]
  115. Saito M, Enomoto M, Tatsuno T. Radiomimetic biological properties of the new scirpene metabolites ofFusarium nivale. Gann. 1969;60:599–603. [PubMed] [Google Scholar]
  116. Samara A, Yagen B, Agranat I, Rachmilewitz EA, Fibach E. Induction of differention in human myeloid leukemic cells by T-2 toxin and other trichothecenes. Toxicol Appl Pharmacol. 1987;89:418–428. doi: 10.1016/0041-008x(87)90161-x. [DOI] [PubMed] [Google Scholar]
  117. Sarr MG, Kelly KA, Phillips SF. Canine jejunal absorption and transit during interdigestive and digestive motor stats. Am J Physiol. 1980;239:G167–G172. doi: 10.1152/ajpgi.1980.239.3.G167. [DOI] [PubMed] [Google Scholar]
  118. Sato N, Ueno Y, Enomoto M. Toxicological approaches to toxic metabolites ofFusaria. VII. Acute and subacute toxicities of T-2 toxin in cats. Jpn J Pharmacol. 1975;25:263–270. doi: 10.1254/jjp.25.263. [DOI] [PubMed] [Google Scholar]
  119. Schiefer HB, Hancock DS. Systemic effects of topical application of T2-toxin in mice. Toxicol Appl Pharmacol. 1984;76:464–472. doi: 10.1016/0041-008x(84)90350-8. [DOI] [PubMed] [Google Scholar]
  120. Schiefer HB, Nicholson S, Kasali OB, Hancock DS, Greenhalgh R. Pathology of acute 3-acetyldeoxynivalenol toxicity in mice. Can J Comp Med. 1985;49:315–318. [PMC free article] [PubMed] [Google Scholar]
  121. Schoental R, Joffe AZ. Lesions induced in rodents by extracts from cultures ofFusarium poae andF sporotrichioides. J Pathol. 1974;112:37–42. doi: 10.1002/path.1711120108. [DOI] [PubMed] [Google Scholar]
  122. Schoental R, Joffe AZ, Yagen B. Cardiovascular lesions and various tumors found in rats given T-2 toxin, a trichothecene metabolite ofFusarium. Cancer Res. 1979;39:2179–2189. [PubMed] [Google Scholar]
  123. Seelig LL, Jr, Billingham RE. Concerning the natural transplantation of maternal leukocytes in milk. Transplant. Proc. 1981;13:1245–1249. [PubMed] [Google Scholar]
  124. Segal R, Milo-Goldzweig I, Joffe AZ, Yagen B. Trichothecene induced hemolysis. The hemolytic activity of T-2 toxin. Toxicol Appl Pharmacol. 1983;70:343–349. doi: 10.1016/0041-008x(83)90152-7. [DOI] [PubMed] [Google Scholar]
  125. Shohami E, Feuerstein G. T-2 toxemia and brain prostaglandins. Prostaglandins. 1986;31:307–319. doi: 10.1016/0090-6980(86)90056-0. [DOI] [PubMed] [Google Scholar]
  126. Siren AL, Feuerstein G. Effect of T-2 toxin on regional blood flow and vascular resistance in the conscious rat. Toxicol Appl Pharmacol. 1986;83:438–444. doi: 10.1016/0041-008x(86)90226-7. [DOI] [PubMed] [Google Scholar]
  127. Skou JC. Enzymatic basis for active transport of Na+ and K+ across cell membrane. Physiol Rev. 1965;45:596–617. doi: 10.1152/physrev.1965.45.3.596. [DOI] [PubMed] [Google Scholar]
  128. Speers GM, Mirocha CJ, Christensen CM, Behrens JC. Effects on laying hens of feeding corn invaded by two species ofFusarium and pure T-2 mycotoxin. Poulty Sci. 1977;56:98–102. doi: 10.3382/ps.0560098. [DOI] [PubMed] [Google Scholar]
  129. Suneja SK, Ram GC, Wagle DS. Effects of feeding T-2 toxin on RNA, DNA and protein contents of liver and intestinal mucosa of rats. Toxicol Lett. 1983;18:73–76. doi: 10.1016/0378-4274(83)90073-5. [DOI] [PubMed] [Google Scholar]
  130. Suneja SK, Ram GC, Wagle DS. Effects of T-2 toxin on glucose and tryptophan uptake and intestinal mucosal enzymes. Toxicon. 1984;22:39–43. doi: 10.1016/0041-0101(84)90136-3. [DOI] [PubMed] [Google Scholar]
  131. Szathmary CI. Trichothecene toxicoses and natural occurrence in Hungary. In: Ueno Y, editor. Trichothecenes: Chemical, Biological and Toxicological Aspects. Amsterdam: Kodansha LTD, Tokyo and Elsevier Scientific Pub BV; 1983. pp. 229–250. [Google Scholar]
  132. Terao K. The target organella of trichothecenes in rodents and poultry. In: Ueno Y, editor. Trichothecenes: Chemical, Biological and Toxicological Aspects. Amsterdam: Kodansha LTD, Tokyo and Elsevier Science Pub BV; 1983. pp. 147–162. [Google Scholar]
  133. Windholz M, Budavari S, Blumetti RF, Ottbein ES, editors. The Merck Index. 10th Ed. Rahway, NJ: Merck and Co Inc; 1983. p. 1400. [Google Scholar]
  134. Thurman JD, Creasia DA, Quance JL, Johnson AJ. Adrenal cortical necrosis caused by T-2 mycotoxicosis in female but not male mice. Am J Vet Res. 1986;47:1122–1124. [PubMed] [Google Scholar]
  135. Tsuchida M, Miura T, Shimizu T, Aibara K. Elevation of thiobarbituric acid values in the rat liver intoxicated by T2 toxin. Biochem Med. 1984;31:146–166. doi: 10.1016/0006-2944(84)90020-6. [DOI] [PubMed] [Google Scholar]
  136. Tung HT, Donaldson WE, Hamilton PB. Effects of aflatoxin on some marker enzymes of lysosomes. Biochim Biophys Acta. 1970;222:665–667. doi: 10.1016/0304-4165(70)90194-7. [DOI] [PubMed] [Google Scholar]
  137. Ueno Y. Trichothecenes: Overview address. In: Rodricks JV, Hesseltine CW, Mehlman MA, editors. Mycotoxins in Human and Animal Health. Park Forest South, IL.: Pathotox Pub Inc; 1977. pp. 189–208. [Google Scholar]
  138. Ueno Y. General toxicology. In: Ueno Y, editor. Trichothecenes: Chemical, Biological and Toxicological Aspects. Amsterdam: Kodansha LTD, Tokyo and Elsevier Science Pub BV; 1983. pp. 135–146. [Google Scholar]
  139. Ueno Y. Toxicological features of T-2 toxin and related trichothecenes. Fund Appl Toxicol. 1984;4:S124–S132. doi: 10.1016/0272-0590(84)90144-1. [DOI] [PubMed] [Google Scholar]
  140. Ueno Y, Fukushima K. Inhibition of protein and DNA synthesis in Ehrlich ascites tumour by Nivalenol, a toxic principle ofFusarium nivale on growing rice. Experientia. 1968;24:1032–1033. doi: 10.1007/BF02138729. [DOI] [PubMed] [Google Scholar]
  141. Ueno Y, Matsumoto H. Inactivation of some thiol-enzymes by trichothecene mycotoxins fromFusarium species. Chem Pharm Bull, Tokyo. 1975;23:2439. doi: 10.1248/cpb.23.2439. [DOI] [PubMed] [Google Scholar]
  142. Ueno Y, Nakajima M, Sakai K, Ishii K, Sato N, Shimada M. Comparative toxicology of trichothecene mycotoxins: Inhibition of protein synthesis in animal cells. Biochem J. 1973;74:285–296. [PubMed] [Google Scholar]
  143. Vesely D, Vesela D, Jelinek R. Nineteen mycotoxins tested on chicken embryos. Toxicol Lett. 1982;13:239–245. doi: 10.1016/0378-4274(82)90218-1. [DOI] [PubMed] [Google Scholar]
  144. Vesonder RF. Natural occurrence in North America. In: Ueno Y, editor. Trichothecene—Chemical, Biological and Toxicological Aspects. Amsterdam: Kodansha Ltd, Tokyo and Elsevier Scientific Pub BV; 1983. pp. 210–217. [Google Scholar]
  145. Visconti A, Mirocha CJ. Identification of various T-2 toxin metabolites in chicken excreta and tissues. Appl Environ Microbiol. 1985;49:1246–1250. doi: 10.1128/aem.49.5.1246-1250.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  146. Vocci MJ, Combs JW, Hillman EA, Resau JH, Trump BF. The cell kinetics of the adaptation of the human esophagus to organ culture. In Vitro. 1983;19:881–891. doi: 10.1007/BF02661708. [DOI] [PubMed] [Google Scholar]
  147. Weaver GA, Kurtz HJ, Mirocha CJ (1977) The effect ofFusarium toxins on food-producing animals. Abstr 81st Annu Mtg US Anim Health Assoc, Minneapolis, MN [PubMed]
  148. Weaver GA, Kurtz HJ, Bates FY, Chi MS, Mirocha CJ, Behrens JC, Robinson TS. Acute and chronic toxicity of T-2 mycotoxin in swine. Vet Rec. 1978;103:531–535. doi: 10.1136/vr.103.24.531. [DOI] [PubMed] [Google Scholar]
  149. Weaver GA, Kurtz HJ, Bates FY, Mirocha CJ, Behrens JC, Hagler WM. Diacetoxyscirpenol toxicity in pigs. Res Vet Sci. 1981;31:131–135. [PubMed] [Google Scholar]
  150. Weaver GA, Kurtz HJ, Mirocha CJ, Bates FY, Behrens JC. Acute toxicity of the mycotosin diacetoxyscirpenol in swine. Can Vet J. 1978;19:267–271. [PMC free article] [PubMed] [Google Scholar]
  151. Weaver GA, Kurtz HJ, Mirocha CJ, Bates FY, Behrens JC, Robison TS. Effect of T-2 toxin on porcine reproduction. Can Vet J. 1978;19:310–314. [PMC free article] [PubMed] [Google Scholar]
  152. Weaver GA, Kurtz HJ, Mirocha CJ, Bates FY, Behrens JC, Robison TS, Gipp WF. Mycotoxin-induced abortions in swine. Can Vet J. 1978;19:72–74. [PMC free article] [PubMed] [Google Scholar]
  153. Weaver GA, Kurtz HJ, Mirocha CJ, Bates FY, Behrens JC, Robison TS, Swanson SP. The failure of purified T-2 mycotoxin to produce hemorrhaging in dairy cattle. Can Vet J. 1980;21:210–213. [PMC free article] [PubMed] [Google Scholar]
  154. Williams PP, Gallagher JE. Preparation and long-term cultivation of porcine tracheal and lung organ cultures by alternate exposure to gaseous and liquid medium phases. InVitro. 1978;14:686–696. doi: 10.1007/BF02616165. [DOI] [PubMed] [Google Scholar]
  155. Williams PP, Hall MR. Swine intestinal explants as a model for the study of viruses and microbial toxins. Food Technol. 1987;41(7):99–101. [Google Scholar]
  156. Williams PP, Hall MR, Rimler RB. Toxin enhanced porcine herpesvirus 1 replication in respiratory/gastrointestinal epithelial cells. Abstr Ann Mtg Am Soc Microbiol. 1987;87:314. [Google Scholar]
  157. Williams PP, Targoswki SP, Rao MVS. Organ culture of piglet intestinal expiants: Growth of viruses, incorporation or methyl-3H-thymidine, and uptake of fluorescein isothiocyanate-labelled colostral cells. Microecol Ther. 1985;15:57–70. [Google Scholar]
  158. Wilson CA, Everard DM, Schoental R. Blood pressure changes and cardiovascular lesions found in rats given T-2 toxin, a trichothecene secondary metabolite of certainFusarium microfungi. Toxicol Lett. 1982;10:35–40. doi: 10.1016/0378-4274(82)90264-8. [DOI] [PubMed] [Google Scholar]
  159. Wyatt RD, Colwell WM, Hamilton PB, Burmeister HR. Neural disturbances in chickens caused by dietary T-2 toxin. Appl Mircobiol. 1973;26:757–761. doi: 10.1128/am.26.5.757-761.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  160. Yagen B, Joffe AZ. Screening of toxic isolates ofFusarium poae andF sporotrichioides involved in causing alimentary toxic aleukia. Appl Environ Microbiol. 1976;32:423–427. doi: 10.1128/aem.32.3.423-427.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  161. Yanagihara T. Cerebral anoxia: Effect on transcription and translation. J Neurochem. 1974;22:113–117. doi: 10.1111/j.1471-4159.1974.tb12186.x. [DOI] [PubMed] [Google Scholar]
  162. Yarom R, More R, Eldor Yagen B. The effects of T-2 toxin on human platelets. Toxicol Appl Pharmacol. 1984;73:210–217. doi: 10.1016/0041-008x(84)90326-0. [DOI] [PubMed] [Google Scholar]
  163. Yarom R, Sherman Y, Bergmann F, Sintov A, Berman LD. T-2 toxin effect on rat aorta: Cellular changesin vivo and growth of smooth muscle cellsin vitro. Exp Molecular Pathol. 1987;47:143–153. doi: 10.1016/0014-4800(87)90069-4. [DOI] [PubMed] [Google Scholar]
  164. Yarom R, Sherman Y, More R, Ginsburg I, Borinsky R, Yagen B. T-2 toxin effect on bacterial infection and leukocyte functions. Toxicol Appl Pharmacol. 1984;75:60–68. doi: 10.1016/0041-008x(84)90076-0. [DOI] [PubMed] [Google Scholar]
  165. Yoshizawa T, Mirocha CJ, Behrens JC, Swanson SP. Metabolic fate of T-2 toxin in a lactating cow. Food Cosmet Toxicol. 1981;19:31–39. doi: 10.1016/0015-6264(81)90300-x. [DOI] [PubMed] [Google Scholar]
  166. Yoshizawa T, Okamoto K, Sakamoto T, Kuwamura K. In vivo metabolism of T-2 toxin, a trichothecene mycotoxin. On the formation of depoxydation products. Proc Jpn Assoc Mycotoxicol. 1985;21:9–12. [Google Scholar]
  167. Yoshizawa T, Sakamoto T, Ayano Y, Mirocha CJ. 3′-hy-droxy T-2 and 3′-hydroxy HT-2 toxins: New metabolites of T-2 toxin, a trichothecene mycotoxin, in animals. Agric Biol Chem. 1982;46:2613–2615. [Google Scholar]
  168. Yoshizawa T, Swanson SP, Mirocha CJ. T-2 metabolites in the excreta of broiler chickens administered3H-labeled T-2 toxin. Appl Environ Microbiol. 1980;39:1172–1177. doi: 10.1128/aem.39.6.1172-1177.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  169. Ziprin RL, Corrier DE, Ziegler HK. LPS escape from T-2 mycotoxin damaged intestine is insufficient to account for T-2 toxin enhanced resistance to listeriosis. Abst Annu Mtg Amer Soc Microbiol. 1987;87:116. [Google Scholar]
  170. Ziprin RL, Corrier DE, Ziegler HK. T-2 toxin-enhanced resistance against listeriosis in mice: importance of gastrointestinal lesions. Am J Vet Res. 1987;48:998–1002. [PubMed] [Google Scholar]

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