Abstract
Animals were immunized with transmissible gastroenteritis virus conjugated with gold nanoparticles. The resultant antibodies had a higher titer than antibodies produced in response to native virus. Immunization with the antigen-colloidal gold complex led to a significant increase of the peritoneal macrophages respiratory activity and of plasma IFN-γ level in immunized animals.
Key Words: gold nanoparticles, transmissible gastroenteritis virus, immunization, macrophages, interferon
Footnotes
Translated from Byulleten’ Eksperimental’noi Biologii i Meditsiny, Vol. 151, No. 4, pp. 418–421, April, 2011
References
- 1.Dykman LA, Bogatyrev VA. Uspekhi Khim. 2007;76(2):199–213. [Google Scholar]
- 2.Dykman LA, Bogatyrev VA. Biokhimiya. 1997;62(4):411–418. [Google Scholar]
- 3.Dykman LA, Sumaroka MV, Staroverov SA, et al. Izv. Akad. Nauk, Ser. Biology. 2004;31(1):86–91. [PubMed] [Google Scholar]
- 4.L. A. Dykman, V. A. Bogatyrev, S. Yu. Shchyogolev, and N. G. Khlebtsov, Gold Nanoparticles: Synthesis, Properties, and Biomedical Use [in Russian], Moscow (2008).
- 5.O. S. Pankratov, T. Z. Baibikov, K. P. Nikolaeva, and S. A. Kukushkin, Method for Detection of Antibodies to Swine Transmissible Gastroenteritis Virus by Neutralization Test on Panels [in Russian], Vladimir (2004).
- 6.Bastus NG, Sanchez-Tillo E, Pujals S, et al. Mol. Immunol. 2009;46(4):743–748. doi: 10.1016/j.molimm.2008.08.277. [DOI] [PubMed] [Google Scholar]
- 7.Bernas T, Dobricki JW. Arch. Biochem. Biophys. 2000;380(1):108–116. doi: 10.1006/abbi.2000.1907. [DOI] [PubMed] [Google Scholar]
- 8.Boisselier E, Astruc D. Chem. Soc. Rev. 2009;38(6):1759–1782. doi: 10.1039/b806051g. [DOI] [PubMed] [Google Scholar]
- 9.Brown CL, Whitehouse MW, Tiekink ER, Bushell GR. Immunopharmacology. 2008;16(3):133–137. doi: 10.1007/s10787-007-0017-6. [DOI] [PubMed] [Google Scholar]
- 10.Dobrovolskaia MA, Mcneil SE. Nat. Nanotechnol. 2007;2(8):469–478. doi: 10.1038/nnano.2007.223. [DOI] [PubMed] [Google Scholar]
- 11.Donnelly JJ, Wahren B, Liu MA. J. Immunol. 2005;175(2):633–639. doi: 10.4049/jimmunol.175.2.633. [DOI] [PubMed] [Google Scholar]
- 12.Frens G. Nature Phys. Sci. 1973;241:20–22. [Google Scholar]
- 13.Ghosh P, Han G, De M, et al. Adv. Drug Deliv. Rev. 2008;60(11):1307–1315. doi: 10.1016/j.addr.2008.03.016. [DOI] [PubMed] [Google Scholar]
- 14.Khlebtsov NG, Bogatyrev VA, Dykman LA, Melnikov AG. J. Coll. Interf. Sci. 1996;180(2):436–445. doi: 10.1006/jcis.1996.0323. [DOI] [Google Scholar]
- 15.Shukla R, Bansal V, Chaudhary M, et al. Langmuir. 2005;21(23):10,644–10,654. doi: 10.1021/la0513712. [DOI] [PubMed] [Google Scholar]