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. 2014 Mar 15;15(2):62–69. doi: 10.1007/s10156-009-0669-9

Loop-mediated isothermal amplification (LAMP): a rapid, accurate, and cost-effective diagnostic method for infectious diseases

Yasuyoshi Mori 1,*, Tsugunori Notomi 1
PMCID: PMC7087713  PMID: 19396514

Abstract

Loop-mediated isothermal amplification (LAMP) is an established nucleic acid amplification method offering rapid, accurate, and cost-effective diagnosis of infectious diseases. This technology has been developed into commercially available detection kits for a variety of pathogens including bacteria and viruses. The current focus on LAMP methodology is as a diagnostic system to be employed in resource-limited laboratories in developing countries, where many fatal tropical diseases are endemic. The combination of LAMP and novel microfluidic technologies such as Lab-on-a-chip may facilitate the realization of genetic point-of-care testing systems to be used by both developed and developing countries in the near future. This review will describe the historical, current, and future developments of such technologies.

Key words: Loop-mediated isothermal amplification (LAMP), Nucleic acid amplification test, Rapid diagnosis tool, Point of care testing (POCT) device

References

  • 1.Cohen M.L. Changing patterns of infectious disease. Nature. 2000;406:762–767. doi: 10.1038/35021206. 10.1038/35021206. [DOI] [PubMed] [Google Scholar]
  • 2.Urdea M., Penny L.A., Olmsted S.S., Giovanni M.Y., Kaspar P., Shepherd A., et al. Requirements for high impact diagnostics in the developing world. Nature. 2006;444:73–79. doi: 10.1038/nature05448. 10.1038/nature05448. [DOI] [PubMed] [Google Scholar]
  • 3.Poxton I.R. Molecular techniques in the diagnosis and management of infectious diseases: do they have a role in bacteriology? Med Princ Pract 14 Suppl. 2005;1:20–26. doi: 10.1159/000086181. 10.1159/000086181. [DOI] [PubMed] [Google Scholar]
  • 4.Gilbert G.L. Molecular diagnostics in infectious diseases and public health microbiology: cottage industry to postgenomics. Trends Mol Med. 2002;8:280–287. doi: 10.1016/s1471-4914(02)02349-3. 10.1016/S1471-4914(02)02349-3. [DOI] [PubMed] [Google Scholar]
  • 5.Hanscheid T., Grobusch M.P. How useful is PCR in the diagnosis of malaria? Trends Parasitol. 2002;18:395–398. doi: 10.1016/s1471-4922(02)02348-6. 10.1016/S1471-4922(02)02348-6. [DOI] [PubMed] [Google Scholar]
  • 6.Ginocchio C.C. Detection of respiratory viruses using non-molecular based methods. J Clin Virol. 2007;40(Suppl.1):S11–S14. doi: 10.1016/S1386-6532(07)70004-5. 10.1016/S1386-6532(07)70004-5. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 7.Curry A., Appleton H., Dowsett B. Application of transmission electron microscopy to the clinical study of viral and bacterial infections: present and future. Micron. 2006;37:91–106. doi: 10.1016/j.micron.2005.10.001. 10.1016/j.micron.2005.10.001. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 8.van Belkum A. Molecular diagnostics in medical microbiology: yesterday, today and tomorrow. Curr Opin Pharmacol. 2003;3:497–501. doi: 10.1016/s1471-4892(03)00108-5. 10.1016/S1471-4892(03)00108-5. [DOI] [PubMed] [Google Scholar]
  • 9.Versalovic J., Lupski J.R. Molecular detection and genotyping of pathogens: more accurate and rapid answers. Trends Microbiol. 2002;10:S15–S21. doi: 10.1016/s0966-842x(02)02438-1. 10.1016/S0966-842X(02)02438-1. [DOI] [PubMed] [Google Scholar]
  • 10.Dong J., Olano J.P., McBride J.W., Walker D.H. Emerging pathogens: challenges and successes of molecular diagnostics. J Mol Diagn. 2008;10:185–197. doi: 10.2353/jmoldx.2008.070063. 10.2353/jmoldx.2008.070063. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 11.Notomi T., Okayama H., Masubuchi H., Yonekawa T., Watanabe K., Amino N., Hase T. Loop-mediated isothermal amplification of DNA. Nucleic Acids Res. 2000;28:E63. doi: 10.1093/nar/28.12.e63. 10.1093/nar/28.12.e63. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 12.Misawa Y., Yoshida A., Saito R., Yoshida H., Okuzumi K., Ito N., et al. Application of loop-mediated isothermal amplification technique to rapid and direct detection of methicillin-resistant Staphylococcus aureus (MRSA) in blood cultures. J Infect Chemother. 2007;13:134–140. doi: 10.1007/s10156-007-0508-9. 10.1007/s10156-007-0508-9. [DOI] [PubMed] [Google Scholar]
  • 13.Eiken Chemical. Eiken genome site. http://loopamp.eiken.co.jp/e/index.html. Accessed. 5 Jan 2009
  • 14.Nagamine K., Hase T., Notomi T. Accelerated reaction by loopmediated isothermal amplification using loop primers. Mol Cell Probes. 2002;16:223–229. doi: 10.1006/mcpr.2002.0415. 10.1006/mcpr.2002.0415. [DOI] [PubMed] [Google Scholar]
  • 15.Mori Y., Kitao M., Tomita N., Notomi T. Real-time turbidimetry of LAMP reaction for quantifying template DNA. J Biochem Biophys Methods. 2004;59:145–157. doi: 10.1016/j.jbbm.2003.12.005. 10.1016/j.jbbm.2003.12.005. [DOI] [PubMed] [Google Scholar]
  • 16.Mori Y., Nagamine K., Tomita N., Notomi T. Detection of loopmediated isothermal amplification reaction by turbidity derived from magnesium pyrophosphate formation. Biochem Biophys Res Commun. 2001;289:150–154. doi: 10.1006/bbrc.2001.5921. 10.1006/bbrc.2001.5921. [DOI] [PubMed] [Google Scholar]
  • 17.Tomita N., Mori Y., Kanda H., Notomi T. Loop-mediated isothermal amplification (LAMP) of gene sequences and simple visual detection of products. Nat Protoc. 2008;3:877–882. doi: 10.1038/nprot.2008.57. 10.1038/nprot.2008.57. [DOI] [PubMed] [Google Scholar]
  • 18.Kaneko H., Kawana T., Fukushima E., Suzutani T. Tolerance of loop-mediated isothermal amplification to a culture medium and biological substances. J Biochem Biophys Methods. 2007;70:499–501. doi: 10.1016/j.jbbm.2006.08.008. 10.1016/j.jbbm.2006.08.008. [DOI] [PubMed] [Google Scholar]
  • 19.LAMP primer designing software. http://primerexplorer.jp/e/. Accessed. 5 Jan 2009
  • 20.Lukinmaa S., Nakari U.M., Eklund M., Siitonen A. Application of molecular genetic methods in diagnostics and epidemiology of food-borne bacterial pathogens. APMIS. 2004;112:908–929. doi: 10.1111/j.1600-0463.2004.apm11211-1213.x. 10.1111/j.1600-0463.2004.apm11211-1213.x. [DOI] [PubMed] [Google Scholar]
  • 21.Tirado C., Schmidt K. WHO surveillance programme for control of foodborne infections and intoxications: preliminary results and trends across greater Europe. World Health Organization. J Infect. 2001;43:80–84. doi: 10.1053/jinf.2001.0861. [DOI] [PubMed] [Google Scholar]
  • 22.Braden C.R. Salmonella enterica serotype Enteritidis and eggs: a national epidemic in the United States. Clin Infect Dis. 2006;43:512–517. doi: 10.1086/505973. 10.1086/505973. [DOI] [PubMed] [Google Scholar]
  • 23.Chen S., Yee A., Griffiths M., Larkin C., Yamashiro C.T., Behari R., et al. The evaluation of a fluorogenic polymerase chain reaction assay for the detection of Salmonella species in food commodities. Int J Food Microbiol. 1997;35:239–250. doi: 10.1016/s0168-1605(97)01241-5. 10.1016/S0168-1605(97)01241-5. [DOI] [PubMed] [Google Scholar]
  • 24.Mullah B., Livak K., Andrus A., Kenney P. Efficient synthesis of double dye-labeled oligodeoxyribonucleotide probes and their application in a real time PCR assay. Nucleic Acids Res. 1998;26:1026–1031. doi: 10.1093/nar/26.4.1026. 10.1093/nar/26.4.1026. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 25.Hara-Kudo Y., Yoshino M., Kojima T., Ikedo M. Loop-mediated isothermal amplification for the rapid detection of Salmonella. FEMS Microbiol Lett. 2005;253:155–161. doi: 10.1016/j.femsle.2005.09.032. 10.1016/j.femsle.2005.09.032. [DOI] [PubMed] [Google Scholar]
  • 26.Ohtsuka K., Yanagawa K., Takatori K., Hara-Kudo Y. Detection of Salmonella enterica in naturally contaminated liquid eggs by loop-mediated isothermal amplification, and characterization of Salmonella isolates. Appl Environ Microbiol. 2005;71:6730–6735. doi: 10.1128/AEM.71.11.6730-6735.2005. 10.1128/AEM.71.11.6730-6735.2005. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 27.Okamura M., Ohba Y., Kikuchi S., Suzuki A., Tachizaki H., Takehara K., et al. M Loop-mediated isothermal amplification for the rapid, sensitive, and specific detection of the O9 group of Salmonella in chickens. Vet Microbiol. 2008;132:197–204. doi: 10.1016/j.vetmic.2008.04.029. 10.1016/j.vetmic.2008.04.029. [DOI] [PubMed] [Google Scholar]
  • 28.Coia J.E. Clinical, microbiological and epidemiological aspects of Escherichia coli O157 infection. FEMS Immunol Med Microbiol. 1998;20:1–9. doi: 10.1111/j.1574-695X.1998.tb01105.x. 10.1016/S0928-8244(97)00105-3. [DOI] [PubMed] [Google Scholar]
  • 29.Hara-Kudo Y., Konishi N., Ohtsuka K., Hiramatsu R., Tanaka H., Konuma H., Takatori K. Detection of Verotoxigenic Escherichia coli O157 and O26 in food by plating methods and LAMP method: a collaborative study. Int J Food Microbiol. 2008;122:156–161. doi: 10.1016/j.ijfoodmicro.2007.11.078. 10.1016/j.ijfoodmicro.2007.11.078. [DOI] [PubMed] [Google Scholar]
  • 30.Yano A., Ishimaru R., Hujikata R. Rapid and sensitive detection of heat-labile I and heat-stable I enterotoxin genes of enterotoxigenic Escherichia coli by loop-mediated isothermal amplification. J Microbiol Methods. 2007;68:414–420. doi: 10.1016/j.mimet.2006.09.024. 10.1016/j.mimet.2006.09.024. [DOI] [PubMed] [Google Scholar]
  • 31.Hill J., Beriwal S., Chandra I., Paul V.K., Kapil A., Singh T., et al. Loop-mediated isothermal amplification assay for rapid detection of common strains of Escherichia coli. J Clin Microbiol. 2008;46:2800–2804. doi: 10.1128/JCM.00152-08. 10.1128/JCM.00152-08. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 32.Yamazaki W., Taguchi M., Ishibashi M., Kitazato M., Nukina M., Misawa N., Inoue K. Development and evaluation of a loop-mediated isothermal amplification assay for rapid and simple detection of Campylobacter jejuni and Campylobacter coli. J Med Microbiol. 2008;57:444–451. doi: 10.1099/jmm.0.47688-0. 10.1099/jmm.0.47688-0. [DOI] [PubMed] [Google Scholar]
  • 33.Inouye S., Yamashita K., Yamadera S., Yoshikawa M., Kato N., Okabe N. Surveillance of viral gastroenteritis in Japan: pediatric cases and outbreak incidents. J Infect Dis 181 Suppl. 2000;2:S270–S274. doi: 10.1086/315593. 10.1086/315593. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 34.Cheng P.K., Wong D.K., Chung T.W., Lim W.W. Norovirus contamination found in oysters worldwide. J Med Virol. 2005;76:593–597. doi: 10.1002/jmv.20402. 10.1002/jmv.20402. [DOI] [PubMed] [Google Scholar]
  • 35.Lopman B.A., Gallimore C., Gray J.J., Vipond I.B., Andrews N., Sarangi J., et al. Linking healthcare associated norovirus outbreaks: a molecular epidemiologic method for investigating transmission. BMC Infect Dis. 2006;6:108. doi: 10.1186/1471-2334-6-108. 10.1186/1471-2334-6-108. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 36.Rabenau H.F., Sturmer M., Buxbaum S., Walczok A., Preiser W., Doerr H.W. Laboratory diagnosis of norovirus: which method is the best? Intervirology. 2003;46:232–238. doi: 10.1159/000072433. 10.1159/000072433. [DOI] [PubMed] [Google Scholar]
  • 37.Green J., Gallimore C.I., Norcott J.P., Lewis D., Brown D.W. Broadly reactive reverse transcriptase polymerase chain reaction for the diagnosis of SRSV-associated gastroenteritis. J Med Virol. 1995;47:392–398. doi: 10.1002/jmv.1890470416. 10.1002/jmv.1890470416. [DOI] [PubMed] [Google Scholar]
  • 38.Greene S.R., Moe C.L., Jaykus L.A., Cronin M., Grosso L., Aarle P. Evaluation of the NucliSens Basic Kit assay for detection of Norwalk virus RNA in stool specimens. J Virol Methods. 2003;108:123–131. doi: 10.1016/S0166-0934(02)00286-0. 10.1016/S0166-0934(02)00286-0. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 39.Ishiguro T., Saitoh J., Horie R., Hayashi T., Ishizuka T., Tsuchiya S., et al. Intercalation activating fluorescence DNA probe and its application to homogeneous quantification of a target sequence by isothermal sequence amplification in a closed vessel. Anal Biochem. 2003;314:77–86. doi: 10.1016/s0003-2697(02)00618-8. 10.1016/S0003-2697(02)00618-8. [DOI] [PubMed] [Google Scholar]
  • 40.Iturriza-Gomara M., Xerry J., Gallimore C.I., Dockery C., Gray J. Evaluation of the Loopamp (loop-mediated isothermal amplification) kit for detecting Norovirus RNA in faecal samples. J Clin Virol. 2008;42:389–393. doi: 10.1016/j.jcv.2008.02.012. 10.1016/j.jcv.2008.02.012. [DOI] [PubMed] [Google Scholar]
  • 41.Fukuda S., Takao S., Kuwayama M., Shimazu Y., Miyazaki K. Rapid detection of norovirus from fecal specimens by real-time reverse transcription-loop-mediated isothermal amplification assay. J Clin Microbiol. 2006;44:1376–1381. doi: 10.1128/JCM.44.4.1376-1381.2006. 10.1128/JCM.44.4.1376-1381.2006. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 42.Yoda T., Suzuki Y., Yamazaki K., Sakon N., Kanki M., Aoyama I., Tsukamoto T. Evaluation and application of reverse transcription loop-mediated isothermal amplification for detection of noroviruses. J Med Virol. 2007;79:326–334. doi: 10.1002/jmv.20802. 10.1002/jmv.20802. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 43.Fukuda S., Sasaki Y., Kuwayama M., Miyazaki K. Simultaneous detection and genogroup-screening test for norovirus genogroups I and II from fecal specimens in single tube by reverse transcription-loop-mediated isothermal amplification assay. Microbiol Immunol. 2007;51:547–550. doi: 10.1111/j.1348-0421.2007.tb03932.x. 10.1111/j.1574-695X.2007.00334.x. [DOI] [PubMed] [Google Scholar]
  • 44.Fukuda S., Sasaki Y., Seno M. Rapid and sensitive detection of norovirus genomes in oysters by a two-step isothermal amplification assay system combining nucleic acid sequencebased amplification and reverse transcription-loop-mediated isothermal amplification assays. Appl Environ Microbiol. 2008;74:3912–3914. doi: 10.1128/AEM.00127-08. 10.1128/AEM.00127-08. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 45.Nishida T., Kimura H., Saitoh M., Shinohara M., Kato M., Fukuda S., et al. Detection, quantitation, and phylogenetic analysis of noroviruses in Japanese oysters. Appl Environ Microbiol. 2003;69:5782–5786. doi: 10.1128/AEM.69.10.5782-5786.2003. 10.1128/AEM.69.10.5782-5786.2003. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 46.Poon L.L., Wong B.W., Chan K.H., Ng S.S., Yuen K.Y., Guan Y., Peiris J.S. Evaluation of real-time reverse transcriptase PCR and real-time loop-mediated amplification assays for severe acute respiratory syndrome coronavirus detection. J Clin Microbiol. 2005;43:3457–3459. doi: 10.1128/JCM.43.7.3457-3459.2005. 10.1128/JCM.43.7.3457-3459.2005. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 47.Hong T.C.T., Mai Q.L., Cuong D.V., Parida M., Minekawa H., Notomi T., et al. Development and evaluation of a novel loop-mediated isothermal amplification method for rapid detection of severe acute respiratory syndrome coronavirus. J Clin Microbiol. 2004;42:1956–1961. doi: 10.1128/JCM.42.5.1956-1961.2004. 10.1128/JCM.42.5.1956-1961.2004. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 48.Taubenberger J.K., Morens D.M. The pathology of influenza virus infections. Annu Rev Pathol. 2008;3:499–522. doi: 10.1146/annurev.pathmechdis.3.121806.154316. 10.1146/annurev.pathmechdis.3.121806.154316. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 49.Morishima T., Togashi T., Yokota S., Okuno Y., Miyazaki C., Tashiro M., Okabe N. Encephalitis and encephalopathy associated with an influenza epidemic in Japan. Clin Infect Dis. 2002;35:512–517. doi: 10.1086/341407. 10.1086/341407. [DOI] [PubMed] [Google Scholar]
  • 50.Matheson NJ, Harnden AR, Perera R, Sheikh A, Symmonds-Abrahams M. Neuraminidase inhibitors for preventing and treating influenza in children. Cochrane Database Syst Rev 2007. 2007;24:CD002744. doi: 10.1002/14651858.CD002744.pub2. [DOI] [PubMed] [Google Scholar]
  • 51.Kawai N., Iwaki N., Kawashima T., Satoh I., Tsuchimoto T., Shigematsu T., et al. Effectiveness of oseltamivir on influenza and influencing factors: age of patients, type of influenza virus and timing of initial administration. Kansenshogaku Zasshi. 2003;77:423–429. doi: 10.11150/kansenshogakuzasshi1970.77.423. [DOI] [PubMed] [Google Scholar]
  • 52.Ito M., Watanabe M., Nakagawa N., Ihara T., Okuno Y. Rapid detection and typing of influenza A and B by loop-mediated isothermal amplification: comparison with immunochromatography and virus isolation. J Virol Methods. 2006;135:272–275. doi: 10.1016/j.jviromet.2006.03.003. 10.1016/j.jviromet.2006.03.003. [DOI] [PubMed] [Google Scholar]
  • 53.Perez D.R., Sorrell E.M., Donis R.O. Avian influenza: an omnipresent pandemic threat. Pediatr Infect Dis J. 2005;24:S208–S216. doi: 10.1097/01.inf.0000188160.83709.b7. 10.1097/01.inf.0000188160.83709.b7. [DOI] [PubMed] [Google Scholar]
  • 54.Imai M., Ninomiya A., Minekawa H., Notomi T., Ishizaki T., Tashiro M., Odagiri T. Development of H5-RT-LAMP (loop-mediated isothermal amplification) system for rapid diagnosis of H5 avian influenza virus infection. Vaccine. 2006;24:6679–6682. doi: 10.1016/j.vaccine.2006.05.046. 10.1016/j.vaccine.2006.05.046. [DOI] [PubMed] [Google Scholar]
  • 55.Imai M., Ninomiya A., Minekawa H., Notomi T., Ishizaki T., Tu P., et al. Rapid diagnosis of H5N1 avian influenza virus infection by newly developed influenza H5 hemagglutinin genespecific loop-mediated isothermal amplification method. J Virol Methods. 2007;141:173–180. doi: 10.1016/j.jviromet.2006.12.004. 10.1016/j.jviromet.2006.12.004. [DOI] [PubMed] [Google Scholar]
  • 56.Jayawardena S., Cheung C.Y., Barr I., Chan K.H., Chen H., Guan Y., et al. Loop-mediated isothermal amplification for influenza A (H5N1) virus. Emerg Infect Dis. 2007;13:899–901. doi: 10.3201/eid1306.061572. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 57.Fujino M., Yoshida N., Yamaguchi S., Hosaka N., Ota Y., Notomi T., Nakayama T. A simple method for the detection of measles virus genome by loop-mediated isothermal amplification (LAMP) J Med Virol. 2005;76:406–413. doi: 10.1002/jmv.20371. 10.1002/jmv.20371. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 58.Hagiwara M., Sasaki H., Matsuo K., Honda M., Kawase M., Nakagawa H. Loop-mediated isothermal amplification method for detection of human papillomavirus type 6, 11, 16, and 18. J Med Virol. 2007;79:605–615. doi: 10.1002/jmv.20858. 10.1002/jmv.20858. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 59.Okafuji T., Yoshida N., Fujino M., Motegi Y., Ihara T., Ota Y., et al. Rapid diagnostic method for detection of mumps virus genome by loop-mediated isothermal amplification. J Clin Microbiol. 2005;43:1625–1631. doi: 10.1128/JCM.43.4.1625-1631.2005. 10.1128/JCM.43.4.1625-1631.2005. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 60.Yoshida N., Fujino M., Miyata A., Nagai T., Kamada M., Sakiyama H., et al. Mumps virus reinfection is not a rare event confirmed by reverse transcription loop-mediated isothermal amplification. J Med Virol. 2008;80:517–523. doi: 10.1002/jmv.21106. 10.1002/jmv.21106. [DOI] [PubMed] [Google Scholar]
  • 61.Karanis P., Thekisoe O., Kiouptsi K., Ongerth J., Igarashi I., Inoue N. Development and preliminary evaluation of a loop-mediated isothermal amplification procedure for sensitive detection of Cryptosporidium oocysts in fecal and water samples. Appl Environ Microbiol. 2007;73:5660–5662. doi: 10.1128/AEM.01152-07. 10.1128/AEM.01152-07. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 62.Annaka T. Rapid and simple detection of Legionella species by LAMP, a new DNA amplification method. Rinsho Biseibutshu Jinsoku Shindan Kenkyukai Shi. 2003;14:25–30. [PubMed] [Google Scholar]
  • 63.Yamazaki W., Seto K., Taguchi M., Ishibashi M., Inoue K. Sensitive and rapid detection of cholera toxin-producing Vibrio cholerae using a loop-mediated isothermal amplification. BMC Microbiol. 2008;8:94. doi: 10.1186/1471-2180-8-94. 10.1186/1471-2180-8-94. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 64.Mabey D., Peeling R.W., Ustianowski A., Perkins M.D. Diagnostics for the developing world. Nat Rev Microbiol. 2004;2:231–240. doi: 10.1038/nrmicro841. 10.1038/nrmicro841. [DOI] [PubMed] [Google Scholar]
  • 65.Keeler E., Perkins M.D., Small P., Hanson C., Reed S., Cunningham J., et al. Reducing the global burden of tuberculosis: the contribution of improved diagnostics. Nature 444 Suppl. 2006;1:49–57. doi: 10.1038/nature05446. [DOI] [PubMed] [Google Scholar]
  • 66.Perkins M.D. New diagnostic tools for tuberculosis. Int J Tuberc Lung Dis. 2000;4:S182–S188. [PubMed] [Google Scholar]
  • 67.Ling D.I., Flores L.L., Riley L.W., Pai M. Commercial nucleic-acid amplification tests for diagnosis of pulmonary tuberculosis in respiratory specimens: meta-analysis and meta-regression. PLoS ONE. 2008;3:e1536. doi: 10.1371/journal.pone.0001536. 10.1371/journal.pone.0001536. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 68.Iwamoto T., Sonobe T., Hayashi K. Loop-mediated isothermal amplification for direct detection of Mycobacterium tuberculosis complex, M. avium, and M. intracellulare in sputum samples. J Clin Microbiol. 2003;41:2616–2622. doi: 10.1128/JCM.41.6.2616-2622.2003. 10.1128/JCM.41.6.2616-2622.2003. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 69.Boehme C.C., Nabeta P., Henostroza G., Raqib R., Rahim Z., Gerhardt M., et al. Operational feasibility of using loop-mediated isothermal amplification for diagnosis of pulmonary tuberculosis in microscopy centers of developing countries. J Clin Microbiol. 2007;45:1936–1940. doi: 10.1128/JCM.02352-06. 10.1128/JCM.02352-06. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 70.Bell D., Wongsrichanalai C., Barnwell J.W. Ensuring quality and access for malaria diagnosis: how can it be achieved? Nat Rev Microbiol. 2006;4:S7–S20. doi: 10.1038/nrmicro1525. 10.1038/nrmicro1525. [DOI] [PubMed] [Google Scholar]
  • 71.Rafael M.E., Taylor T., Magill A., Lim Y.W., Girosi F., Allan R. Reducing the burden of childhood malaria in Africa: the role of improved. Nature 444 Suppl. 2006;1:39–48. doi: 10.1038/nature05445. 10.1038/nature05445. [DOI] [PubMed] [Google Scholar]
  • 72.Guerin P.J., Olliaro P., Nosten F., Druilhe P., Laxminarayan R., Binka F., et al. Malaria: current status of control, diagnosis, treatment, and a proposed agenda for research and development. Lancet Infect Dis. 2002;2:564–573. doi: 10.1016/s1473-3099(02)00372-9. 10.1016/S1473-3099(02)00372-9. [DOI] [PubMed] [Google Scholar]
  • 73.Erdman L.K., Kain K.C. Molecular diagnostic and surveillance tools for global malaria control. Travel Med Infect Dis. 2008;6:82–99. doi: 10.1016/j.tmaid.2007.10.001. 10.1016/j.tmaid.2007.10.001. [DOI] [PubMed] [Google Scholar]
  • 74.Andrews L., Andersen R.F., Webster D., Dunachie S., Walther R.M., Bejon P., et al. Quantitative real-time polymerase chain reaction for malaria diagnosis and its use in malaria vaccine clinical trials. Am J Trop Med Hyg. 2005;73:191–198. [PubMed] [Google Scholar]
  • 75.Han E.T., Watanabe R., Sattabongkot J., Khuntirat B., Sirichaisinthop J., Iriko H., et al. Detection of four Plasmodium species by genus- and species-specific loop-mediated isothermal amplification for clinical diagnosis. J Clin Microbiol. 2007;45:2521–2528. doi: 10.1128/JCM.02117-06. 10.1128/JCM.02117-06. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 76.Snounou G. Rapid, sensitive and cheap molecular diagnosis of malaria: is microscopy on the way out? Future Microbiol. 2007;2:477–480. 10.2217/17460913.2.5.477. [Google Scholar]
  • 77.Curtis K.A., Rudolph D.L., Owen S.M. Rapid detection of HIV-1 by reverse-transcription, loop-mediated isothermal amplification (RT-LAMP) J Virol Methods. 2008;151:264–270. doi: 10.1016/j.jviromet.2008.04.011. 10.1016/j.jviromet.2008.04.011. [DOI] [PubMed] [Google Scholar]
  • 78.Boutayeb A. Developing countries and neglected diseases: challenges and perspectives. Int J Equity Health. 2007;6:20. doi: 10.1186/1475-9276-6-20. 10.1186/1475-9276-6-20. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 79.Fairlamb A.H. Chemotherapy of human African trypanosomiasis: current and future prospects. Trends Parasitol. 2003;19:488–494. doi: 10.1016/j.pt.2003.09.002. 10.1016/j.pt.2003.09.002. [DOI] [PubMed] [Google Scholar]
  • 80.Chappuis F., Loutan L., Simarro P., Lejon V., Buscher P. Options for field diagnosis of human African trypanosomiasis. Clin Microbiol Rev. 2005;18:133–146. doi: 10.1128/CMR.18.1.133-146.2005. 10.1128/CMR.18.1.133-146.2005. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 81.Kuboki N., Inoue N., Sakurai T., Cello F., Grab D.J., Suzuki H., et al. Loop-mediated isothermal amplification for detection of African trypanosomes. J Clin Microbiol. 2003;41:5517–5524. doi: 10.1128/JCM.41.12.5517-5524.2003. 10.1128/JCM.41.12.5517-5524.2003. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 82.Thekisoe O.M., Kuboki N., Nambota A., Fujisaki K., Sugimoto C., Igarashi I., et al. Species-specific loop-mediated isothermal amplification (LAMP) for diagnosis of trypanosomosis. Acta Trop. 2007;102:182–189. doi: 10.1016/j.actatropica.2007.05.004. 10.1016/j.actatropica.2007.05.004. [DOI] [PubMed] [Google Scholar]
  • 83.Njiru Z.K., Mikosza A.S., Armstrong T., Enyaru J.C., Ndung’u J.M., Thompson A.R. Loop-mediated isothermal amplification (LAMP) method for rapid detection of Trypanosoma brucei rhodesiense. PLoS Negl Trop Dis. 2008;2:e147. doi: 10.1371/journal.pntd.0000147. 10.1371/journal.pntd.0000147. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 84.Njiru Z.K., Mikosza A.S., Matovu E., Enyaru J.C., Ouma J.O., Kibona S.N., et al. African trypanosomiasis: sensitive and rapid detection of the sub-genus Trypanozoon by loop-mediated isothermal amplification (LAMP) of parasite DNA. Int J Parasitol. 2008;38:589–599. doi: 10.1016/j.ijpara.2007.09.006. 10.1016/j.ijpara.2007.09.006. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 85.Poon L.L., Wong B.W., Ma E.H., Chan K.H., Chow L.M., Abeyewickreme W., et al. Sensitive and inexpensive molecular test for falciparum malaria: detecting Plasmodium falciparum DNA directly from heat-treated blood by loop-mediated isothermal amplification. Clin Chem. 2006;52:303–306. doi: 10.1373/clinchem.2005.057901. 10.1373/clinchem.2005.057901. [DOI] [PubMed] [Google Scholar]
  • 86.Parida M., Horioke K., Ishida H., Dash P.K., Saxena P., Jana A.M., et al. Rapid detection and differentiation of dengue virus serotypes by a real-time reverse transcription-loop-mediated isothermal amplification assay. J Clin Microbiol. 2005;43:2895–2903. doi: 10.1128/JCM.43.6.2895-2903.2005. 10.1128/JCM.43.6.2895-2903.2005. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 87.Kurosaki Y., Takada A., Ebihara H., Grolla A., Kamo N., Feldmann H., et al. Rapid and simple detection of Ebola virus by reverse transcription-loop-mediated isothermal amplification. J Virol Methods. 2007;141:78–83. doi: 10.1016/j.jviromet.2006.11.031. 10.1016/j.jviromet.2006.11.031. [DOI] [PubMed] [Google Scholar]
  • 88.Parida M.M., Santhosh S.R., Dash P.K., Tripathi N.K., Lakshmi V., Mamidi N., et al. Rapid and real-time detection of Chikungunya virus by reverse transcription loop-mediated isothermal amplification assay. J Clin Microbiol. 2007;45:351–357. doi: 10.1128/JCM.01734-06. 10.1128/JCM.01734-06. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 89.Parida M., Posadas G., Inoue S., Hasebe F., Morita K. Real-time reverse transcription loop-mediated isothermal amplification for rapid detection of West Nile virus. J Clin Microbiol. 2004;42:257–263. doi: 10.1128/JCM.42.1.257-263.2004. 10.1128/JCM.42.1.257-263.2004. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 90.Rodger A.J., Cooke G.S., Ord R., Sutherland C.J., Pasvol G. Cluster of falciparum malaria cases in UK airport. Emerg Infect Dis. 2008;14:1284–1286. doi: 10.3201/eid1408.080031. 10.3201/eid1408.080031. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 91.Greer A., Ng V., Fisman D. Climate change and infectious diseases in North America: the road ahead. CMAJ. 2008;178:715–722. doi: 10.1503/cmaj.081325. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 92.Whitesides G.M. The origins and the future of microfluidics. Nature. 2006;442:368–373. doi: 10.1038/nature05058. 10.1038/nature05058. [DOI] [PubMed] [Google Scholar]
  • 93.Kopp M.U., Mello A.J., Manz A. Chemical amplification: continuous-flow PCR on a chip. Science. 1998;280:1046–1048. doi: 10.1126/science.280.5366.1046. 10.1126/science.280.5366.1046. [DOI] [PubMed] [Google Scholar]
  • 94.Lee S.Y., Huang J.G., Chuang T.L., Sheu J.C., Chuang Y.K., Hall M., et al. Compact optical diagnostic device for isothermal nucleic acids amplification. Sens Actuators B Chem. 2008;133:493–501. doi: 10.1016/j.snb.2008.03.008. 10.1016/j.snb.2008.03.008. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 95.Lee C.Z., Huang G.T., Yang P.M., Sheu J.C., Lai M.Y., Chen D.S. Correlation of HBV DNA levels in serum and liver of chronic hepatitis B patients with cirrhosis. Liver. 2002;22:130–135. doi: 10.1034/j.1600-0676.2002.01525.x. 10.1034/j.1600-0676.2002.01525.x. [DOI] [PubMed] [Google Scholar]
  • 96.Hataoka Y., Zhang L., Mori Y., Tomita N., Notomi T., Baba Y. Analysis of specific gene by integration of isothermal amplification and electrophoresis on poly (methylmethacrylate) microchips. Anal Chem. 2004;76:3689–3693. doi: 10.1021/ac035032u. 10.1021/ac035032u. [DOI] [PubMed] [Google Scholar]
  • 97.Burns M.A., Johnson B.N., Brahmasandra S.N., Handique K., Webster J.R., Krishnan M., et al. An integrated nanoliter DNA analysis device. Science. 1998;282:484–487. doi: 10.1126/science.282.5388.484. 10.1126/science.282.5388.484. [DOI] [PubMed] [Google Scholar]
  • 98.Iseki H., Alhassan A., Ohta N., Thekisoe O.M., Yokoyama N., Inoue N., et al. Development of a multiplex loop-mediated isothermal amplification (mLAMP) method for the simultaneous detection of bovine Babesia parasites. J Microbiol Methods. 2007;71:281–287. doi: 10.1016/j.mimet.2007.09.019. 10.1016/j.mimet.2007.09.019. [DOI] [PubMed] [Google Scholar]
  • 99.Nakamura N., Ito K., Takahashi M., Hashimoto K., Kawamoto M., Yamanaka M., et al. Detection of six single-nucleotide polymorphisms associated with rheumatoid arthritis by a loop-mediated isothermal amplification method and an electrochemical DNA chip. Anal Chem. 2007;79:9484–9493. doi: 10.1021/ac0715468. 10.1021/ac0715468. [DOI] [PubMed] [Google Scholar]
  • 100.Mori Y., Hirano T., Notomi T. Sequence specific visual detection of LAMP reactions by addition of cationic polymers. BMC Biotechnol. 2006;6:3. doi: 10.1186/1472-6750-6-3. 10.1186/1472-6750-6-3. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 101.Tani H., Teramura T., Adachi K., Tsuneda S., Kurata S., Nakamura K., et al. Technique for quantitative detection of specific DNA sequences using alternately binding quenching probe competitive assay combined with loop-mediated isothermal amplification. Anal Chem. 2007;79:5608–5613. doi: 10.1021/ac070041e. 10.1021/ac070041e. [DOI] [PubMed] [Google Scholar]

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