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. 2007 Feb 16;73(1):66–71. doi: 10.1007/s10327-006-0316-6

Immunochromatographic assay for simple and rapid detection of Satsuma dwarf virus and related viruses using monoclonal antibodies

Nario Kusano 1,✉, Keita Hirashima 1, Minoru Kuwahara 1, Kenji Narahara 2, Tadashi Imamura 2, Tomohiro Mimori 2, Ken Nakahira 2, Kuniaki Torii 2
PMCID: PMC7087811  PMID: 32214869

Abstract

A simple and rapid immunochromatographic assay (ICA) to detect Satsuma dwarf virus (SDV) was developed using colloidal gold conjugates of anti-SDV monoclonal antibodies. Of six homogenization buffers tested, 0.1 M citrate buffer (pH 7.0) gave the best results for the ICA. In the ICA, addition of 0.1% thioglycolic acid in the homogenization buffers that have been widely used in enzyme-linked immunosorbent assays (ELISA) was deleterious to the reaction because of undesirable coagulation of the colloidal gold. ICA using the anti-SDV monoclonal antibodies was 8 times and 16 times more sensitive than double antibody sandwich-ELISA and ICA using the anti-SDV polyclonal antibody, respectively. The analysis is complete in only 15 min. Furthermore, ICA using the anti-SDV monoclonal antibodies could also detect SDV-related viruses.

Key words: Satsuma dwarf virus, Detection, Immunochromatographic assay, ELISA

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