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. 2005;27(13):883–891. doi: 10.1007/s10529-005-7176-6

Expression and Purification of N and E Proteins from Severe Acute Respiratory Syndrome (SARS)-Associated Coronavirus: a Comparative Study

Chia-Wei Lai 1, Yao-Chi Chung 1, Yiu-Kay Lai 2, Margaret Dah-Tsyr Chang 2, Yu-Chen Hu 1,✉
PMCID: PMC7087856  PMID: 16091881

Abstract

Histidine-tagged N (rNH) and E (rEH) proteins of Severe Acute Respiratory Syndrome (SARS)-coronovirus were expressed in the baculovirus/insect cell system and purified by immobilized metal affinity chromatography. rNH and rEH proteins differed markedly with respect to expression levels, cell death kinetics and subcellular localizations that led to different extraction and purification schemes. The features of both proteins are compared and the potential applications of purified rNH and rEH are discussed.

Keywords: baculovirus, coronavirus, immobilized metal affinity chromatography, Severe Acute Respiratory Syndrome virus

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