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. 2006 Aug 16;28(20):1661–1666. doi: 10.1007/s10529-006-9141-4

Expression of Helicobacter pylori urease subunit B gene in transgenic rice

Qing Gu 1,2,✉, Ning Han 1, Jianyi Liu 3, Muyuan Zhu 1
PMCID: PMC7088001  PMID: 16912927

Abstract

Helicobacter pylori ureB antigen gene was cloned to the 5′-end of gus (β-glucuronidase) reporter gene between CaMV35S promoter and the octopine synthase (OCS) terminator in the plasmid, pCAMBIA13011. It was then introduced into rice genome by Agrobacterium-mediated transformation. A total of 30 regenerated plants with hygromycin resistance were obtained in the selection media. The putative transgenic individuals were tested for the presence of ureB in the nuclear genome of rice plants by PCR analysis. Expression of ureB gene in rice plants was verified by RT-PCR and Western blot analysis using polyclonal human antiserum for transcription and translation levels respectively. These results provide a basis for further studies on the accumulation level of UreB recombinant protein in transgenic rice and potential utilization of transgenic rice for delivery of edible vaccines against Helicobacter pylori.

Keywords: Helicobacter pylori, Transgenic rice, ureB, Vaccine

Acknowledgments

This work was supported by the National Natural Science Foundation of China (No.30300017; 30370876), the Natural Science Foundation of Zhejiang Province (No.301018), and the program for science and technology from Zhejiang Province (No.2004C32015; 2003C34005).

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