Abstract
Polyacrylamide gel electrophoresis of nucleic acid extracted from stool samples of diarrhoeic children revealed in 3 out of 690 (0.43 %) specimens two electrophoretic bands with a migration pattern characteristic of picobirnavirus ds-RNA. In none of the 92 control children were similar bands detected. No other potential enteric pathogens were found in the patients with picobirnavirus infection.
Key words: Picobirnavirus, Gastroenteritis, PAGE
References
- 1.Adkins HJ, Escamilla J, Santiago LT. Two-yeas survey of etiologic agents of diarrhoeal disease at San Lazaro Hospital, Manila, Republic of Philippines. J Clin Microbiol. 1987;25:1143–1147. doi: 10.1128/jcm.25.7.1143-1147.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
- 2.Arista S, Giovannelli L, Titone L. Detection of an antigenically distinct human rotavirus in Palermo. Ann Inst Pasteur Virol. 1985;136E:229–235. [Google Scholar]
- 3.Bates PR, Bailey AS, Wood DJ. Comparative epidemiology of rotavirus, subgenus F (types 40 and 41) adenovirus, and astrovirus gastroenteritis in children. J Med Virol. 1993;3:224–228. doi: 10.1002/jmv.1890390309. [DOI] [PubMed] [Google Scholar]
- 4.Brown D, Gallimore C, Appleton H. Bisegmented ds viruses (Picobirnaviruses) in man: preliminary studies. VIIIth International Congress of Virology. Berlin, Germany 1990; Abstract: 344.
- 5.Grohmann GS, Glass RI, Pereira HG. Enteric viruses and diarrhea in HIV-infected patients. N Eng J Med. 1993;329:14–20. doi: 10.1056/NEJM199307013290103. [DOI] [PubMed] [Google Scholar]
- 6.Herring AJ, Inglis NF, Ojeh CK. Rapid diagnosis of rotavirus infection by direct detection of viral nucleic acid in silver-stained polyacrylamide gels. J Clin Microbiol. 1982;16:473–477. doi: 10.1128/jcm.16.3.473-477.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
- 7.Ludert JE, Liprandi F. Identification of viruses with bi- and trisegmented double-stranded RNA genome in faeces of children with gastroenteritis. Res Virol. 1993;144:219–224. doi: 10.1016/s0923-2516(06)80032-4. [DOI] [PubMed] [Google Scholar]
- 8.Monroe S, Glass R, Noah N. Electron microscopic reporting of gastrointestinal viruses in the United Kingdom 1985–1987. J Med Virol. 1991;33:193–198. doi: 10.1002/jmv.1890330310. [DOI] [PMC free article] [PubMed] [Google Scholar]
- 9.Pereira HG, Flewett TH, Candeias JA. A virus with a bisegmented double-stranded RNA genome in rat (oryzomys nigripes) intestines. J Gen Virol. 1988;69:2749–2754. doi: 10.1099/0022-1317-69-11-2749. [DOI] [PubMed] [Google Scholar]
- 10.Pereira HG, Fiahlo AM, Flewett TH. Novel viruses in human faeces. Lancet. 1988;9:103–104. doi: 10.1016/s0140-6736(88)90032-3. [DOI] [PubMed] [Google Scholar]
- 11.Yolken R, Dubovi E, Leister F, et al. Infantile gastroenteritis associated with excretion of pestivirus antigens. Lancet 1989 (ii): 517–520. [DOI] [PubMed]