Abstract
We investigated the effect of exogenous mouse α-+β-interferon produced by mouse L cells on the growth of mouse hepatitis virus type 2 (MHV-2) in the liver, the development of liver cell necrosis, and survival in murine fulminant hepatitis induced by MHV-2. Murine fulminant hepatitis was induced in 4-week-old male ICR mice by intraperitoneal inoculation of MHV-2. Mouse interferon (103 IU/mouse/day) was intraperitoneally injected every day. Exogenous mouse interferon suppressed both the growth of MHV-2 in the liver tissue and development of liver cell necrosis, and prolonged the survival. It was also found that the earlier mouse interferon was administered, the greater was the prolongation of survival.
Keywords: Hepatic Tissue, Fulminant Hepatitis, Mouse Hepatitis Virus, Liver Cell Necrosis, Intraperitoneal Inoculation
References
- 1.Stewart WE II, editor. The Interferon System. New York: Springer-Verlag; 1979. [Google Scholar]
- 2.Levin S, Hahn T. Interferon system in acute viral hepatitis. Lancet. 1982;1:592–594. doi: 10.1016/S0140-6736(82)91751-2. [DOI] [PubMed] [Google Scholar]
- 3.Levy AG, Leibowitz J, Edington ST. Lymphocyte-instructed monocyte induction of the coagulation pathways parallels the induction of hepatitis by the murine hepatitis virus. In: Popper H, Shaffner F, editors. Progress in Liver Disease, Vol 7. New York: Grune & Stratton; 1982. pp. 393–409. [PubMed] [Google Scholar]
- 4.Taguchi F, Hirano N, Kiuchi Y, Fujiwara K. Difference in response to mouse hepatitis virus among susceptible mouse strains. Jpn J Microbiol. 1976;20:293–302. doi: 10.1111/j.1348-0421.1976.tb00991.x. [DOI] [PubMed] [Google Scholar]
- 5.Hirano N, Murakami T, Taguchi F, Fujiwara K, Matsumoto M. Comparison of mouse hepatitis virus strains for pathogenicity in weanling mice infected by various routes. Arch Virol. 1981;70:69–73. doi: 10.1007/BF01320795. [DOI] [PMC free article] [PubMed] [Google Scholar]
- 6.Hirano N, Fujiwara K, Hirano S. Replication and plaque formation of mouse hepatitis virus (MHV-2) in mouse cell line DRT culture. Arch Gesamte Virusforch. 1974;44:298–302. doi: 10.1007/BF01240618. [DOI] [PubMed] [Google Scholar]
- 7.Utumi J, Iizuka M, Kobyashi S. Interferon production with multitray culture system on a large scale. J Interferon Res. 1984;4:9–16. doi: 10.1089/jir.1984.4.9. [DOI] [PubMed] [Google Scholar]
- 8.Gehan E. Generalized Wilcoxon test for comparing arbitrarily singly-censored samples. Biometrika. 1956;52:203–224. [PubMed] [Google Scholar]
- 9.Virelizier JL, Gresser I. Role of interferon in the pathogenesis of viral disease of mice as demonstrated by the use of antiinterferon serum. V. Protective role in mouse hepatitis virus type 3 infection of susceptible and resistant strains of mice. J Immunol. 1978;120:1616–1619. [PubMed] [Google Scholar]
- 10.Schindler L, Engler H, Kirchner H. Activation of natural killer cells and induction of interferon after injection of mouse hepatitis virus type 3 in mice. Infect Immun. 1982;35:869–873. doi: 10.1128/iai.35.3.869-873.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
- 11.Levy AG, MacPhee JP, Fung SL, Fisher MM, Rappaport MA. The effect of mouse hepatitis virus infection on the microcirculation of the liver. Hepatology. 1983;3:964–973. doi: 10.1002/hep.1840030614. [DOI] [PMC free article] [PubMed] [Google Scholar]
- 12.Bang FB, Warwick A. Mouse macrophages as host cells for the mouse hepatitis virus and the genetic basis of their susceptibility. Proc Natl Acad Sci USA. 1960;46:1065–1075. doi: 10.1073/pnas.46.8.1065. [DOI] [PMC free article] [PubMed] [Google Scholar]
- 13.Weiser WY, Bang FB. Blocking ofin vitro andin vivo susceptibility to mouse hepatitis virus. Exp Med. 1977;146:1467–1472. doi: 10.1084/jem.146.5.1467. [DOI] [PMC free article] [PubMed] [Google Scholar]