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. 1984;3(2):136–140. doi: 10.1007/BF02014331

Comparison of six methods for detecting human rotavirus in stools

F Morinet 1, F Ferchal 1, R Colimon 1, Y Pérol 1,✉
PMCID: PMC7088116  PMID: 6327296

Abstract

The following six methods for detecting rotavirus in human faecal samples were compared: electron microscopy, immune electron microscopy, immunofluorescence in cell culture, two enzyme immunoassays (Rotazyme, Enzygnost) and a latex agglutination test (Rotalex). Specimens were collected from 112 children with diarrhoea. The relative sensitivities of the different assays for human rotavirus were as follows: electron microscopy, 84%; immunofluorescence, 86%; Rotalex, 88%; Rotazyme, 89%; immune electron microscopy, 93%; Enzygnost, 98%. According to our findings Enzygnost is the most sensitive method, but Rotalex is more valuable for screening a small number of faecal samples. No false-positive results were observed in the two enzyme immunoassays or in Rotalex.

Keywords: Electron Microscopy, Cell Culture, Internal Medicine, Faecal Sample, Enzyme Immunoassay

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