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. 1990;76(8):648–652. doi: 10.1007/BF00931081

An improved ELISA for the detection of antibodies againstBabesia bovis using either a native or a recombinantB. bovis antigen

R Böse 1,✉, R H Jacobson 2, K R Gale 1, D J Waltisbuhl 1, I G Wright 1
PMCID: PMC7088413  PMID: 2251239

Abstract

Two new enzyme-linked immunosorbent assayes (ELISA) for the diagnosis ofBabesia bovis in cattle are described. The ELISA using a native antigen is more sensitive and less laborious than the assays described previously, because it does not require adsorption of sera with bovine erythrocytes. The second ELISA, using a recombinantB. bovis antigen expressed inEscherichia coli, was both sensitive and specific. It is suitable to replace the native antigen, thus avoiding large batch-to-batch variations in antigen preparations and the need to sacrifice experimental cattle.

Keywords: Immunosorbent Assayes, Antigen Preparation, Bovine Erythrocyte, Native Antigen, inEscherichia Coli

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