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. 2004;15(4):295–297. doi: 10.1007/BF02844956

Serological survey on canine coronavirus antibodies in giant pandas by virus neutralization test

Qiao Jun 1,2,✉, Xia Xian-zhu 1, Yang Song-tao 1, Li De-sheng 3, Hu Gui-xue 4, Gao Yu-wei 1, Sun He-ting 1, Zhao Zhong-pen 1, Xie Zhi-jing 1, Yan Fang 1, He Wen-qi 1, Huang Gen 1
PMCID: PMC7088830  PMID: 32214748

Abstract

In order to survey the infectious situation of canine coronavirus (CCV) in giant panda population, a virus neutralization test detecting specific antibodies against CCV in giant panda’s sera was established by using two-fold dilutions of serum and 100 TCID50 of the virus. The 62 sera samples of giant pandas, which were gathered from zoos and reserve region of Sichuan Province, China were detected. The neutralization antibody titer of 1:4 was recognized as the positive criterion, 8 sera samples were detected to be positive, and the positive rate was 12.9%. The titers of neutralizing antibody ranged from 1:8 to 1:32. It was the first comprehensive investigation on neutralization antibodies against CCV in giant panda population in China. The results of study showed that the infection of CCV in giant panda population was universal, which has posed a threat to the health of giant panda. Therefore, it is incumbent on us to study safe and effective vaccines to protect giant panda against CCV infection.

Keywords: Serological survey, Canine coronavirus, Giant panda, Neutralization test

Footnotes

Foundation item: This research was supported by National Science Foundation of China (No. 30000123) and Conversation Department of Wildlife Animal & Plants of State Forestry Bureau.

References

  1. Appel M., et al. Canine coronavirus [C] In: Appel M J, et al., editors. Virus infections of carnivores I. Amsterdam. Elsevier: Sciences Publishers BV; 1987. pp. 115–122. [Google Scholar]
  2. Bandai C., Ishiguro S., Masuya N., et al. Canine coronavirus infections in Japan: virological and epidemiological aspects. J. Vet. Med. Sci. 1999;61(7):731–736. doi: 10.1292/jvms.61.731. [DOI] [PubMed] [Google Scholar]
  3. Binn L. N., Lazar E. C., Keenan K. P., et al. Recovery and characterization of a coronavirus from military dogs with diarrhea [J] Proceedings of the Annual Meeting of the US Animal Health Assoc. 1974;78:359–366. [PubMed] [Google Scholar]
  4. Evermann J.F., Foreyt W., Maag-Miller L., et al. Acute hemorrhagic enteritis associated with canine coronavirus and parvovirus infection in a captive coyote population [J] J. Am. Vet. Med. Assoc. 1980;177(9):784–786. [PubMed] [Google Scholar]
  5. Fensan Gao, Guixue Hu, Xianzhu Xia, et al. Diagnosis of complex infection of canine distemper virus and canine coronavirus to giant panda by multi-PCR [J] Chinese Journal of Jilin Agricultural University. 2003;25(I):91–93. [Google Scholar]
  6. Aihua He, Shengfang Du, Guihua Ling, et al. Mini report on detection of coronavirus in feces of giant panda [J] Chinese Journal of zoonosis. 1996;4(3):4–4. [Google Scholar]
  7. Keenan K.P., Jervis H.R., Marchwicki R.H., et al. Intestinal infection of neonatal dogs with canine coronavirus 1–71: studies by virologic, histologie, histochemical, and immunofluorescent techniques [J] Am. J. Vet. Res. 1976;37(3):247–256. [PubMed] [Google Scholar]
  8. Kelly D.F., Carter S.D., Gaskell C.J. Canine coronavirus infection in the dog following oronasal inoculation [J] Res. Vet. Sci. 1991;51(1):11–18. doi: 10.1016/0034-5288(91)90023-H. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Mainka S. A., Qiu X., He T., et al. Serologie survey of giant panda (Ailuropoda melanoleuca), and domestic dogs and cats in the Wolong Reserve, China [J] Journal of Wildlife Disease. 1994;30(1):86–89. doi: 10.7589/0090-3558-30.1.86. [DOI] [PubMed] [Google Scholar]
  10. Pratelli A., Buonavoglia D., Martella V., et al. Diagnosis of canine coronavirus infection using nested-PCR [J] J.Virol. Meth. 2000;84(1):91–94. doi: 10.1016/S0166-0934(99)00134-2. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Pratelli A., Tempesta M., Greco G., et al. Development of a nested PCR assay for the detection of canine coronavirus [J] J. Virol. Meth. 1999;80(1):11–15. doi: 10.1016/S0166-0934(99)00017-8. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Jun Qiao, Xianzhu Xia, Guixue Hu, et al. Cloning and sequence analysis of spike protein gene of canine coronavirus giant panda’s isolate [J] Virologica. Sinica. 2004;19(5):481–486. [Google Scholar]
  13. Rimmelzwaan G.F., Groen J., Egberink H., et al. The use of enzyme-linked immunosorbent assay systems for serology and antigen detection of parvovirus, coronavirus and rotavirus interactions in dogs in The Netherlands [J] Vet. Microbiol. 1991;26(3):25–40. doi: 10.1016/0378-1135(91)90039-I. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Takeuchi A., Binn L.N., Jervis H.R., et al. Electronmicroscope study of experimental enteric infection in neonatal dogs with a canine coronavirus [J] Lab. Invest. 1976;34(6):539–549. [PubMed] [Google Scholar]
  15. Tennant B. J., Gaskell R. M., Jones R. C., et al. Studies on the epizootiology of canine coronavirus [J] Veterinary Record. 1993;132(2):7–11. doi: 10.1136/vr.132.1.7. [DOI] [PubMed] [Google Scholar]
  16. Tennant B.J., Gaskell R.M., Tresnan D.B., et al. Feline aminopeptidase N is a receptor for all group I coronaviruses [J] Adv. Exp. Med. 1998;440:69–75. doi: 10.1007/978-1-4615-5331-1_9. [DOI] [PubMed] [Google Scholar]
  17. Yasoshima A., Fujinami F., Doi K., et al. Case report on mixed infection of canine parvovirus and canine coronavirus. Electron microscopy and recovery of canine coronavirus [J] Jpn. J. Vet. Sci. 1983;45(2):217–25. doi: 10.1292/jvms1939.45.217. [DOI] [PubMed] [Google Scholar]

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