Abstract
A multiplex polymerase chain reaction (PCR) for identification of four viruses causing acute respiratory diseases in human beings was developed. The analytical sensitivity of developed RT-PCR for identification of adenovirus, respiratory-syncytial virus, flu viruses types A and B, and actual subtypes of type A flu virus (seasonal and pandemic variants H1N1, seasonal H3N2, and viruses of bird flu that are pathogenic to human beings H5 and H7) was 1 × 103 genome equivalents per milliliter. Diagnostic sensitivity for flu virus type A and B, and also subtypes H1 (seasonal H1N1, pandemic variant of H1N1 of year 2009), H3, H5 was 1 × 103–104 viral particles per milliliter. The method developed has high specificity and does not have positive signal in experiments with DNA/cDNA of human beings and viral DNA. We have studied 50 samples using the developed set. Etiology was defined in 33 samples.
Keywords: multiplex PCR, diagnosis of respiratory infections, flu virus, respiratory-syncytial virus, adenovirus, acute respiratory infection (ARI)
Footnotes
Original Russian Text © E.I. Sergeeva, V.A. Ternovoi, O.K. Demina, A.V. Demina, D.V. Korneev, A.N. Shikov, S.A. Beryllo, A.P. Agafonov, A.N. Sergeev, 2013, published in Molekulyarnaya Genetika, Mikrobiologiya i Virusologiya, 2013, No. 4, pp. 32–37.
Contributor Information
E. I. Sergeeva, Email: esergeeva@vector.nsc.ru
V. A. Ternovoi, Email: tern@vector.nsc.ru
O. K. Demina, Email: demina@nsc.ru
A. V. Demina, Email: Deminaanna@mail.ru
D. V. Korneev, Email: korneev_dv@vector.nsc.ru
A. N. Shikov, Email: shikov@vector.nsc.ru
S. A. Beryllo, Email: berillo@vector.nsc.ru
A. P. Agafonov, Email: agafonov@vector.nsc.ru
A. N. Sergeev, Email: an_sergeev@vector.nsc.ru
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